Tea polysaccharide-chromium complex as well as preparation and application thereof
A technology of tea polysaccharide and chromium complex, which is applied in the field of medicine, can solve the problems such as the result that the effect cannot be achieved, and achieve the effects of high development value, no toxic side effects, and small adverse reactions.
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[0031] 1 Purification of tea crude polysaccharides
[0032] 1.1 Purification of tea polysaccharides
[0033] Dissolve 10 g of tea crude polysaccharide solution in 100 ml of water and 0.25% trypsin solution according to the volume ratio of 1 (tea crude polysaccharide solution): 0.4 (trypsin solution) and mix evenly, place in a constant temperature water bath at 37°C and heat at constant temperature for 3 hours, then Add 28ml of 10wt% H at pH=7.0 to this mixture 2 o 2 Reaction 12h;
[0034] Then put the tea crude polysaccharide solution after enzymolysis into boiling water to extinguish the fire enzyme for 10 minutes, place it at room temperature to cool, then discard the precipitate by high-speed centrifugation, take 168ml of the supernatant and put it in a beaker, add 42ml of sevage solution, and stir under magnetic force Stir vigorously on the instrument for 20 minutes, pour into a separatory funnel and let stand to separate layers, discard the protein layer and the organi...
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