31 results about "Hyperglycemic Mouse" patented technology

The invention discloses a total triterpenoid extract of inonotus obliquus with a hypoglycemic function, which is prepared through the following steps: (1) crushing and sieving inonotus obliquus, adding ethanol aqueous solution for immersion, carrying out ultrasonic extraction, separating the supernatant, and concentrating and drying the supernatant, thereby obtaining ethanol extract; (2) dispersing the ethanol extract into distilled water, oscillating and extracting with petroleum ether, and separating the extract from water-phase residues generated in extraction; and (3) oscillating and extracting the water-phase residues with n-butanol, separating the extract, mixing, concentrating and drying, thereby obtaining the total triterpenoid extract of the inonotus obliquus with the hypoglycemic function, wherein the content of the triterpenoid is 70-300mg/g. the total triterpenoid extract of the inonotus obliquus with the hypoglycemic function provided by the invention can obviously reduce the fasting blood glucose level of mouse with hyperglycemia, can significantly ease the diabetes symptoms of the mouse with hyperglycemia, and has no significant toxicity to major organs.

The invention relates to a process flow to produce a sesquiterpene inside a golden fungus fermentation liquid and the virtue of the sesquiterpene produced by the golden fungus to reduce the blood sugar, belonging to the biological fermentation and medicine field. The process flow is characterized in that the process flow applicant finds the fermentation liquid and the sesquiterpene produced by the production process are applied on a high blood sugar mouse through an oral administration route, the mouse significantly reduces the blood sugar and the fructosamine, which approves that the golden fungus fermentation liquid and the sesquiterpene are possessed of preparing a health care drink and medicine to reduce the blood sugar; the fermentation liquid color is orange to yellow-brown; the inhibitive rate to the ex vivo non-enzymic glycosylation is up to 72% to 90% with the fermentation liquid and 5mg/ml sesquiterpene. The process flow to produce a sesquiterpene inside a golden fungus fermentation liquid and the virtue of the sesquiterpene produced by the golden fungus to reduce the blood sugar has the advantages of not only creating a fire-new channel to further develop the golden fungus but also providing an ideal Chinese herbal medicine to reduce blood sugar.

The invention discloses a preparation method of mollugin. The preparation method includes: taking India madder root, and using 5-15 times of petroleum ether to perform heating reflux extraction for 20-40min; recycling petroleum ether, and vacuum drying to remove petroleum ether so as to obtain extract; and subjecting the extract to 200-300-mesh silica gel column chromatography, eluting with petroleum ether, performing TLC (thin layer chromatography) detection, combining components rich in mollugin, subjecting to Sephadex LH-20 gel column chromatography, eluting with mixture of chloroform, petroleum ether, and methanol in mass ratio of 5:5:1, subjecting to silica gel H-column decompression column chromatography, eluting with petroleum ether, and recrystalizing with hot ethanol to obtain the mollugin. The invention further discloses application of the mollugin in preparation of hypoglycemic drugs and preparation of drugs for treating diabetes. Experiments show that the mollugin can effectively reduce blood sugar, TC (total cholesterol), TG (total glycerin) and MDA (methylene dioxyamphetamine) content of mice with high blood sugar caused by alloxan, and can evidently increase SOD (superoxide dismutase) activity and hepatic glycogen content of the mice.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus paracasei with the effects of reducing blood sugar, protecting the oral cavity and resisting depression and application of the lactobacillus paracasei. The lactobacillus paracasei nbk-LC16 provided by the invention has relatively strong acid resistance and cholate resistance, is good in tolerance in artificial gastric juice and artificial intestinal juice, and can effectively reduce blood sugar and inhibit streptococcus mutans. The influence of the lactobacillus paracasei nbk-LC16 on hyperglycemia mice is studied, and after the lactobacillus paracasei nbk-LC16 is perfused, compared with model group mice, the weight of the hyperglycemia mice is remarkably increased in 8W, the liver index and the spleen index are remarkably reduced, FBG and PBG are remarkably reduced, the HbA1c and insulin levels are remarkably reduced, and the hepatoglycogen content is increased. Test results show that the lactobacillus paracasei nbk-LC16 can regulate blood glucose by regulating insulin level and HbA1c and promoting conversion of blood glucose into hepatic glycogen. Researches on the inhibitioneffect of the streptococcus mutans strain on streptococcus mutans show that the streptococcus mutans strain can be effectively inhibited through in-vitro tests and oral test results, and the oral cavity and tooth health can be protected; meanwhile, the anti-depression effect is achieved.

The invention relates to the activity of golden camellia tea for assisting in lowering blood sugar, the capsule of golden camellia for assisting in lowering blood sugar and a preparation method thereof. The prescription of golden camellia tea auxiliary hypoglycemic capsule is: 33%-66% of golden camellia tea extract, 67%-34% of medicinal excipients. The preparation method is as follows: taking golden camellia tea extract and medicinal auxiliary materials according to the weight percentage of the prescription, and processing them into hard capsules. Among them, golden camellia tea extract refers to the extract obtained by soaking, extracting, and concentrating the golden camellia tea leaves with an appropriate solvent; pharmaceutical excipients refer to medicinal starch or dextrin or a mixture of the two; solvent refers to water or ethanol or a mixture of both. Jinhua tea capsules have a significant hypoglycemic effect on mice with hyperglycemia induced by alloxan. The golden camellia tea capsule of the present invention and its function of assisting in lowering blood sugar. There is no report before this, and this is found by the inventor of this patent through experiments, therefore, special application for patent protection.

The invention discloses a pharmaceutical composition for resisting diabetes activity and a preparation method thereof. The composition is composed of an olea europaea leaf extract and a carrier, wherein the carrier is selected from starch, beta-cyclodextrin, a talcum powder, microcrystalline cellulose and magnesium stearate. In-vitro cell glucose consumption experiments prove that the composition increases the glucose consumption amount of model cells and has obvious hypoglycemic effect. In-vivo animal experiments prove that the composition has a significant role in lowering blood glucose on mesoxyalyurea induced hyperglycemic mice.

The invention discloses stereum hirsutum fermentation liquor and sesquiterpene which are produced by fermenting stereum hirsutum liquor, as well as applications thereof, and particularly relates to a production technology process of sesquiterpene cultured and obtained by the stereum hirsutum liquor, and the hypoglycemic efficiency of sesquiterpene. Repeated tests and study show that a culture solution produced by the production technology contains sesquiterpene, a mouse with hyperglycemia takes the culture solution orally, and then blood sugar and fructosamine of the mouse can be reduced remarkably, therefore, the stereum hirsutum culture solution is confirmed to have the application for preparing healthy drinks and medicines capable of reducing the blood sugar. The culture solution is liquor with the color ranging from aurantium to tawny, and the sesquiterpene content in the culture solution is 0.4 mg/mL-4.0 mg/mL. According to the invention, not only is a new way explored for the deep development and utilization of the stereum hirsutum, but also an ideal traditional Chinese medicine is provided for controlling the blood sugar.

The invention discloses a preparation method and application of fructus lycii polysaccharide-chromium complex. In the fructus lycii polysaccharide-chromium complex, mass fraction of chromium is 5-7%. Purified fructus lycii polysaccharide and chromium are self-assembled to react to form complex under certain condition, and the complex is separated and purified to obtain the fructus lycii polysaccharide-chromium complex. According to the animal activity test, the fructus lycii polysaccharide-chromium complex remarkably reduce hypoglycemic action of hyperglycemic mice, and the hypoglycemic action reduction is higher than that of inorganic chromium and fructus lycii polysaccharide, and is further superior to that of glibenclamide under certain dose. Therefore, the fructus lycii polysaccharide-chromium complex can be used for preparing an antidiabetic drug and healthcare food.

The invention relates to a catalpol-containing medicine composition for reducing blood glucose and a preparation method and application thereof. After the catalpol is singly used, or the catalpol is compounded with other active components, such as one or multiple of sulfonylurea, metformin, alpha-glycosidase inhibitors, insulin sensitization agents, glenn urea medicines or traditional Chinese medicine compositions, the excellent effect of reducing blood glucose, triglyceride, cholesterol and glycated hemoglobin in hyperglycemia mice or rats is realized; the catalpol-containing medicine composition can be widely applied to prevent and treat the diabetes, especially type 2 diabetes.

The invention discloses a method for preparing gynura medica tartary buckwheat germ bean sprout powder. The method consists of the puffing powder preparation, raw material preparation and processing technology, the technical problems of the common food process are solved, and multiple biological tests are carried out on physiological activity and pharmacological effects of gynura medica. The result shows that (1) the gynura medica alcohol extract and aqueous extract have the obvious effects of reducing blood sugar in adrenaline hyperglycemia and exogenous glucose hyperglycemia and are superior to glybenzcyclamide; (2) the gynura medica has the function of promoting insulin secretion; (3) the clinically common glybenzcyclamide is adopted in a control group, the blood sugar of the mouse is reduced to a normal value or below, the hypoglycaemia reaction can be generated, the gynura medica can reduce the blood sugar to a normal level and does not have obvious influence on the blood sugar of the mouse, and a hypoglycaemia side effect is avoided; (4) the sugar reducing effect of each dosage of the gynura medica does not present a dose-effect relationship; and (5) the gynura medica is extremely low in toxicity and is safely taken orally. According to the method for preparing the gynura medica tartary buckwheat germ bean sprout powder, the requirements of vast patients on multiple options of treatment of diabetes can be met.

The invention relates to a traditional Chinese medicine composition for treating diabetes. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 5-10 parts of jerusalem artichoke, 5-8 parts of panax notoginseng, 3-8 parts of white paeony root, 5-8 parts of gypsum, 3-5 parts of astragalus membranaceus, 2-3 parts of bitter gourd, 2-3 parts of trichosanthes kirilowi root, 2-3 parts of rhizoma anemarrhenae, 2-3 parts of radix rehmanniae, 2-3 parts of dendrobium, 2-3 parts of scutellaria baicalensis and 2-3 parts of goldthread root; a capsule or a tablet is prepared by a conventional preparation technology. The traditional Chinese medicine composition has a significant effect on improving the organism immunity, coordinating the viscera functions, fundamentally improving the physiological functions of diabetes patients and restoring normal functions of pancreas islet beta cells; in an animal test, the traditional Chinese medicine composition has an obvious effect on lowering epinephrine hyperglycaemia of alloxan model hyperglycemic mice, and has a recovery effect on pancreatic tissue damages caused by mesoxyalyurea; and in a clinical test, the effect of treating both symptoms and root causes can be reached on diabetes and complications of diabetes.

The invention discloses application of esculentoside A in reducing blood glucose. According to the application disclosed by the invention, an STZ high blood glucose mouse model is established to inspect a blood glucose reducing effect of the esculentoside on high blood glucose mice. An empty-stomach mouse gavage method is used for the esculentoside, and a tail-amputation blood taking blood glucosemeter detection method is used for blood glucose level detection. A blank control group, an esculentoside standard substance experimental group, an esculentoside extractive group in which molding andgavage are performed at the same time, a mouse high blood glucose model group and a melbine experimental group are arranged. Four indexes of weight, water intake, food intake and blood glucose levelare observed. The dosage range of the esculentoside is 0.100 mg/ml to 0.200 mg/ml. After the esculentoside is applied to gavage, the blood glucose level of the high blood glucose mice is reduced to the range between 6.7 to 10.9 from the range between 11.0 and 25.0 within 28 days; thus, a blood glucose reducing effect is obvious. Furthermore, the esculentoside can obviously improve the situations of peeing more, eating more, drinking more and less weight.

The invention discloses a tea polysaccharide-chromium complex as well as preparation and application thereof. The complex is a complex formed by carrying out coordination reaction on purified tea polysaccharide and chromium under a certain condition, separating and purifying. An animal activity experiment proves that the tea polysaccharide-chromium complex has a remarkable effect of lowering blood glucose of hyperglycemia mice; the effect of lowering the blood glucose of the complex is stronger than that of the inorganic chromium and the tea polysaccharide, so that the tea polysaccharide-chromium complex can be used for preparing anti-diabetes medicines and health-care foods.

The invention provides a health-care food or medical composition having the effect of reducing blood sugar. The health-care food or medical composition is prepared from the following raw materials inparts by weight: 10-50 parts of amorphophallus konjac powder, 5-40 parts of mulberry leaves and 5-40 parts of radix puerariae. The invention further provides a preparation method and use of the composition. The composition provided by the invention is reasonable in compatibility, all the components exert the synergetic effects, the postprandial blood sugar of a mouse suffering from high blood sugar can be significantly reduced, and the application prospect is good.

The invention discloses a preparation method of hibiscus sabdariffa fermented milk for reducing blood sugar. The preparation method comprises the following steps of extracting a water extract from hibiscus sabdariffa, and fermenting composite milk materials of the hibiscus sabdariffa and remade milk with a mixed strain fermenting agent so as to obtain the hibiscus sabdariffa fermented milk. The utilization rate of the hibiscus sabdariffa is increased, and besides, the function of the hibiscus sabdariffa fermented milk for reducing blood sugar is evaluated. The hibiscus sabdariffa fermented milk obtained by the preparation method disclosed by the invention is notable in the function of reducing blood sugar for mice suffering from high blood sugar induced by high-fat feeds and streptozotocin, the main technical indexes of the hibiscus sabdariffa fermented milk conform to the relevant regulation of the national standard GB19302-2010, and the content of viable counts in the hibiscus sabdariffa fermented milk is slightly high. The fermented milk is rich in nutrition and rich in fragrance and can balance intestinal flora.

The invention discloses application of ruscogenin in preparing a blood sugar reduction medicine and relates to the technical field of natural medicines. Experiments show that ruscogenin has a remarkable blood sugar reduction function on a mouse suffering from hyperglycemia induced by high fat, is capable of remarkably reducing the level of blood sugar of a mouse suffering from diabetes, and has the main mechanism that utilization and conversion of glucose are intensified by promoting absorption of glucose in muscle tissue, so that formation of HbA1c can be interfered, and the blood sugar of the mouse suffering from hyperglycemia can be reduced. Therefore, the ruscogenin has good application prospects in preparing diabetes medicines and medicine compositions of the diabetes medicines. The ruscogenin is simple and convenient in extraction method, wide in source, small in side effect and wide in application. The whole screening operation is simple, the method is convenient and rapid, anda theoretic basis can be provided for further development of the ruscogenin in the field of diabetes.

An application of a Hangzhou chrysanthemum extract product in preparation of healthcare foods and medicines for preventing and treating hyperglycemia is disclosed. The Hangzhou chrysanthemum extract product is prepared into a medicine for animals and is administrated to mice by perfusion. Influences of the medicine prepared from the Hangzhou chrysanthemum extract product on blood glucose of normal mice, influences of the medicine on the glucose tolerance of normal mice, and influences of different concentrations of the medicine on glucose of mice with hyperglycemia induced by alloxan are measured. Results show that: long-term taking of the Hangzhou chrysanthemum extract product can reduce the blood glucose concentration of the normal mice, and reduce the blood glucose concentration of the mice with hyperglycemia induced by glucose and the alloxan. The Hangzhou chrysanthemum extract product can be used for healthcare foods and medicines for preventing and treating the hyperglycemia.