Golden fungus fermentation liquor or sesquiterpenes produced by fermentation of golden fungus liquid

A sesquiterpene and fermentation liquid technology, applied in the field of biological fermentation and medicine, can solve the problem of no secondary metabolite sesquiterpenoids

Inactive Publication Date: 2010-07-07
章克昌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the medical efficacy of golden ear, some scholars have carried out in-depth research on golden ear from nutritional factors such as culture medium, culture conditions and polysaccharides, but there is no report on secondary metabolites and sesquiterpenoids

Method used

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  • Golden fungus fermentation liquor or sesquiterpenes produced by fermentation of golden fungus liquid
  • Golden fungus fermentation liquor or sesquiterpenes produced by fermentation of golden fungus liquid
  • Golden fungus fermentation liquor or sesquiterpenes produced by fermentation of golden fungus liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: the tissue separation of golden ear fungus species

[0056] Take the fresh unopened fruiting body of Yunnan wild golden ear, cut off the base of the stipe and the outer skin of the stipe, disinfect the surface of the fruiting body with 75% ethanol, move it to a super-clean workbench, and irradiate it with ultraviolet light for 20 minutes. Burn the scalpel on the flame of an alcohol lamp and put it in sterile water to cool down to ensure that the temperature of the scalpel will not damage the fruiting body tissue. Divide the fruiting body into two, cut the epidermal tissue in the stipe (or in the stipe), the junction of the stipe and the cap, the cap and the gills, small pieces the size of soybeans, and do not cut through the surface of the fruiting body when cutting organize. Use sterile tweezers to pick up the slant culture medium (that is, PDA medium) of the test tube, pick up 10 of each tissue, culture in the dark at 25-28°C, and regularly observe the ...

Embodiment 2

[0057] The preparation of embodiment 2 golden ear fermented liquid

[0058] 1. In the newly prepared potato culture medium (Zhuge Jian, Wang Zhengxiang, Industrial Microbiology Experimental Technical Handbook, 1994: P367), insert the golden ear fungus classification that embodiment 1 obtains, culture temperature 27 ℃, treat that mycelium is overgrown After the slant, the slant strains were inserted into 250 mL Erlenmeyer flasks containing 60 mL of shaking flask culture medium (5 bottles in total), and cultured on a shaker at 27° C. and 150 rpm for 24 hours. Wherein the formula of shake flask culture medium is (unit is gram / liter): glucose 5, corn flour 15, soybean meal powder 5, KH 2PO 4 3. MgSO 4 1. The initial pH value is 6.3.

[0059] 2. Insert the above shake flask strains into 500mL Erlenmeyer flasks equipped with 150mL expansion medium (24 flasks in total), the inoculation amount is to insert 11mL shake flask seeds into each 500mL Erlenmeyer flask, and shake at 27°C a...

Embodiment 3

[0063] The preparation of embodiment 3 sesquiterpene crude product

[0064] The acidity of the fermented liquid obtained in Example 2 was adjusted to pH 1.0, and after centrifugation at 3000rpm for 30 minutes, the supernatant was adsorbed with nonpolar macroporous resin AB-8, and the flow rate was 1 / 20 column volume / min. After the end, the resin column was washed with a large amount of water until it was colorless, and then eluted with 20% ethanol solution. The eluate was concentrated in vacuo, and the concentrated solution was freeze-dried to obtain a crude sesquiterpene. The crude product is yellow or light yellow powder, soluble in 40% methanol and ethanol aqueous solution, hot water, pyridine, alkaline solution, and shaking after hot water dissolves to produce relatively persistent foam. Its composition is:

[0065]

[0066] The dry weight of the finally obtained sesquiterpene composition was 2.8 g / L, and the inhibition rate of sesquiterpene at a concentration of 5 mg / ...

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Abstract

The invention relates to a process flow to produce a sesquiterpene inside a golden fungus fermentation liquid and the virtue of the sesquiterpene produced by the golden fungus to reduce the blood sugar, belonging to the biological fermentation and medicine field. The process flow is characterized in that the process flow applicant finds the fermentation liquid and the sesquiterpene produced by the production process are applied on a high blood sugar mouse through an oral administration route, the mouse significantly reduces the blood sugar and the fructosamine, which approves that the golden fungus fermentation liquid and the sesquiterpene are possessed of preparing a health care drink and medicine to reduce the blood sugar; the fermentation liquid color is orange to yellow-brown; the inhibitive rate to the ex vivo non-enzymic glycosylation is up to 72% to 90% with the fermentation liquid and 5mg / ml sesquiterpene. The process flow to produce a sesquiterpene inside a golden fungus fermentation liquid and the virtue of the sesquiterpene produced by the golden fungus to reduce the blood sugar has the advantages of not only creating a fire-new channel to further develop the golden fungus but also providing an ideal Chinese herbal medicine to reduce blood sugar.

Description

technical field [0001] The present invention relates to the fields of biological fermentation and medicine. Specifically, the present invention relates to a method for producing auricularia fermented liquid or sesquiterpene by fermenting auricularia aureus, the auricium auricum fermented liquid or sesquiterpene crude product obtained by the method, and the fermented Liquid or sesquiterpene crude pharmaceutical composition and / or health care product. The auricularia fermented liquid or sesquiterpene crude product has multiple medicinal functions, and can be used to prepare medicines for preventing and / or treating diabetes, cardiovascular and cerebrovascular diseases or bronchitis, especially it has hypoglycemic effect, making it a Blood sugar provides ideal medicine. Background technique [0002] Diabetes is a common and frequently-occurring disease among middle-aged and elderly people. The complications caused by diabetes seriously endanger people's life and health. The ex...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/07C12N1/14C12P5/02A61P3/10
Inventor 章克昌张志才丁重阳顾正华王玉红
Owner 章克昌
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