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Rubia extract and its preparation method and application

A technology of extract and madder, applied in the field of madder extract and its preparation and application, can solve the problem of no research report on pharmacological activity

Inactive Publication Date: 2012-02-01
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pharmacological studies have shown that alizarin has a significant antibacterial effect, but there is no research report on other pharmacological activities of this compound

Method used

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  • Rubia extract and its preparation method and application
  • Rubia extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This example is the extraction method of Rubia extract——ethyl acetate part, which specifically includes the following steps: take the dry product of Rubia, pulverize it, use 95% alcohol for percolation and extraction at room temperature, recover ethanol, and obtain the extract; dissolve the extract In water, heated to dissolve it completely; then extracted with ethyl acetate and concentrated to obtain ethyl acetate extract.

Embodiment 2

[0024] This embodiment is the separation method of the compound alizarin, which is the separation product of the ethyl acetate extract of Rubiae, which comprises the following steps: the ethyl acetate extract is subjected to 200-300 mesh silica gel column chromatography, and petroleum ether-acetone gradient elution (95 :5~9:1), 4 parts were obtained, the third part was eluted by silica gel H column chromatography, petroleum ether-acetone (15:1), combined by TLC detection, and washed by Sephadex LH-20 column chromatography, acetone Take off to obtain the compound alizarin.

Embodiment 3

[0026] This example is an experiment of treating diabetes with the ethyl acetate extract of Rubiae and the compound alizarin.

[0027] METHODS: Mouse Alloxan Diabetes Model

[0028] Principle: Alloxan is an islet β cell toxic agent, which can selectively damage the islet β cells of various animals and cause diabetes. It is the most commonly used animal model of diabetes because of its simple method and high formation rate. For the stability study of the alloxan mouse diabetes model, mainly through the alloxan solution, the diabetes is induced after injection, and the blood glucose level in the mouse serum is detected within a certain period of time. In diabetes, the GSP (glycosylated serum protein) content, which reflects the average blood sugar level in the past 1-2 weeks and the impact of drugs on non-enzymatic glycation in the body, will increase. At the same time, the activity of certain enzymes in the process of glycogen synthesis in the body will be low. Decreased glyc...

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Abstract

The present invention relates to a madder extract and its preparation method and application, wherein the madder extract is prepared by the following method: crushing the rhizome of madder, extracting by percolating with ethanol at room temperature; recovering the ethanol to obtain an extract; extracting the extract Dissolved in water, heated to dissolve completely; then extracted with ethyl acetate, concentrated to obtain Rubia ethyl acetate extract. Experiments show that the extract of Rubia ethyl acetate and the compound alizarin can effectively reduce the blood glucose level, GSP value, TG value and MDA content in tissue homogenate of mice with hyperglycemia induced by alloxan, and can significantly increase the SOD activity value of hepatocytes and increase tissue liver glycogen content. It is proved that Rubia ethyl acetate extract and alizarin have good hypoglycemic effect.

Description

technical field [0001] The invention relates to a preparation method of ethyl acetate extract of madder grass and its application in the preparation of drugs for lowering blood sugar and treating diabetes; in particular, it relates to the application of alizarin, an isolated compound of the part, in preparing drugs for lowering blood sugar and treating diabetes. Background technique [0002] Diabetes mellitus is a systemic disease characterized by hyperglycemia, metabolic disorders, and closely related to insulin secretion. It is a clinical syndrome caused by the interaction of genetic and environmental factors. Absolute or relative insufficiency of insulin secretion and decreased sensitivity of target tissues to insulin lead to a series of metabolic disorders of sugar, protein, fat, water and electrolytes. Diabetes is mainly divided into two types: type I diabetes (ie, insulin-dependent diabetes) and type II diabetes (ie, non-insulin-dependent diabetes), the latter accounti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/74A61K125/00A61K31/122A61P3/10
Inventor 康文艺张丽宋艳丽
Owner HENAN UNIVERSITY
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