Lactobacillus paracasei and application thereof

A kind of Lactobacillus, paracheese technology, applied in the field of microorganisms

Active Publication Date: 2020-08-21
诺佰克(武汉)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The object of the present invention is to provide a strain of Lactobacillus paracasei with the effect of lowering blood sugar and protecting the oral cavity and its application. After taking it for a period of time, it can regulate and control blood sugar, achieve the effect of lowering blood sugar, and solve the health problems caused by taking a large number of hypoglycemic drugs. At the same time, it can effectively inhibit Streptococcus mutans and promote the growth of other beneficial bacteria such as lactic acid bacteria to protect oral health

Method used

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  • Lactobacillus paracasei and application thereof
  • Lactobacillus paracasei and application thereof
  • Lactobacillus paracasei and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The preparation method of embodiment 1 Lactobacillus paracasei nbk-LC16 bacterium powder

[0067] The culture medium and lyoprotectant used in this embodiment are as follows:

[0068] In terms of mass percentage, the composition of the seed medium is: 3% glucose, 2% peptone, 1.5% yeast extract powder, 1% beef extract, 0.5% anhydrous sodium acetate, 0.05% magnesium sulfate, 0.06% manganese sulfate, 0.1% spit Temperature 80 and the rest of the water, the pH value is 6.8;

[0069]In terms of mass percentage, the composition of the fermentation medium is: 2.3% glucose, 2.5% soybean peptone, 2% yeast extract powder, 0.6% anhydrous sodium acetate, 0.06% sodium alginate, 0.05% magnesium sulfate, 0.03% manganese sulfate, 0.1 % Tween 80 and the balance water.

[0070] In terms of mass percentage, the freeze-drying protective agent includes: 10% trehalose, 8% skimmed milk powder, 0.5% glycerin and the balance of water.

[0071] 1) Lactobacillus paracasei nbk-LC16 was inoculate...

Embodiment 2

[0078] Example 2 Acid-resistant and bile-salt-resistant characteristic research of Lactobacillus paracasei nbk-LC16

[0079] (1) the Lactobacillus paracasei nbk-LC16 bacterium powder that embodiment 1 obtains is respectively inoculated with the inoculum size of 1% (w / v) in the MRS substratum that pH is 6.0 and pH is 37 DEG C and cultivates 3h after adopting Viable bacteria were counted by plate counting method, and the results are shown in Table 1.

[0080] Table 1 Research on acid resistance of Lactobacillus paracasei nbk-LC16

[0081]

[0082] It can be seen that under the condition of pH 3.0, Lactobacillus paracasei nbk-LC16 still has a sufficient number of viable bacteria to pass through the gastric environment after 3 hours.

[0083] (2) the Lactobacillus paracasei nbk-LC16 bacterium powder that embodiment 1 obtains is connected with the inoculum size of 1% (w / v) respectively and be that 0% (w / v) and bile salt concentration are 0.3% The viable bacteria were counted b...

Embodiment 3

[0092] Example 3 Lactobacillus paracasei nbk-LC16 hypoglycemic research in vitro

[0093] The tertiary seed liquid of Lactobacillus paracasei nbk-LC16 was centrifuged for 10 min at a speed of 4 000 r / min (4°C), the supernatant was poured off to get the precipitate, and the PBS solution (concentration 0.1 mol / L, pH = 6.8) Resuspend after washing 2 to 3 times, uniformly adjust the density of the bacterial suspension to 1×10 9 CFU / mL, to prepare bacterial suspension. The determination method is as follows:

[0094] Determination of α-glucosidase inhibition rate: In a system with a total volume of 205 μL, add 50 μL of PBS solution (concentration 0.1mol / L, pH=6.8), and then add 50 μL of p-nitrophenol-α with a concentration of 20 mmol / L -D-glucopyranoside (PNPG) solution and 25 μL of the sample to be tested, incubate the mixture at 37°C for 10 minutes, add 30 μL of α-glucosidase solution 20 U / mL to continue the reaction for 20 minutes, add 50 μL of 1mol / L Na 2 CO 3 As the reacti...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus paracasei with the effects of reducing blood sugar, protecting the oral cavity and resisting depression and application of the lactobacillus paracasei. The lactobacillus paracasei nbk-LC16 provided by the invention has relatively strong acid resistance and cholate resistance, is good in tolerance in artificial gastric juice and artificial intestinal juice, and can effectively reduce blood sugar and inhibit streptococcus mutans. The influence of the lactobacillus paracasei nbk-LC16 on hyperglycemia mice is studied, and after the lactobacillus paracasei nbk-LC16 is perfused, compared with model group mice, the weight of the hyperglycemia mice is remarkably increased in 8W, the liver index and the spleen index are remarkably reduced, FBG and PBG are remarkably reduced, the HbA1c and insulin levels are remarkably reduced, and the hepatoglycogen content is increased. Test results show that the lactobacillus paracasei nbk-LC16 can regulate blood glucose by regulating insulin level and HbA1c and promoting conversion of blood glucose into hepatic glycogen. Researches on the inhibitioneffect of the streptococcus mutans strain on streptococcus mutans show that the streptococcus mutans strain can be effectively inhibited through in-vitro tests and oral test results, and the oral cavity and tooth health can be protected; meanwhile, the anti-depression effect is achieved.

Description

Technical field: [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Lactobacillus paracasei with functions of lowering blood sugar, protecting oral cavity and antidepressant and application thereof. Background technique: [0002] Diabetes is a chronic disease in which the relative or absolute deficiency of insulin secretion in the body leads to metabolic disorders in the body. It is recognized as the third largest chronic disease in the world. Diabetic patients are often accompanied by multiple complications, such as retinopathy, renal arteriosclerosis, and cardiovascular and cerebrovascular diseases, which seriously threaten people's health and even life. With the continuous improvement of people's living standards, the incidence of diabetes is also increasing rapidly. According to the survey results of the International Diabetes Federation, the number of people with diabetes in the world was 240 million in 2007, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23K10/18A61K35/747A61P3/10A61P1/02A61P31/04A61P25/24C12R1/225
CPCC12N1/20A23L33/135A23K10/18A61K35/747A61P3/10A61P1/02A61P31/04A61P25/24A23V2002/00C12R2001/225C12N1/205A23V2400/165A23V2200/328A23V2200/312A23V2200/322
Inventor 张阳湛剑龙张丽姣
Owner 诺佰克(武汉)生物科技有限公司
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