A strain of Lactobacillus paracasei and its application

A technology of Lactobacillus and paracheese, applied in the field of microorganisms, to achieve the effects of lowering blood sugar levels, inhibiting Streptococcus mutans, and improving body weight

Active Publication Date: 2021-11-09
诺佰克(武汉)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The object of the present invention is to provide a strain of Lactobacillus paracasei with the effect of lowering blood sugar and protecting the oral cavity and its application. After taking it for a period of time, it can regulate and control blood sugar, achieve the effect of lowering blood sugar, and solve the health problems caused by taking a large number of hypoglycemic drugs. At the same time, it can effectively inhibit Streptococcus mutans and promote the growth of other beneficial bacteria such as lactic acid bacteria to protect oral health

Method used

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  • A strain of Lactobacillus paracasei and its application
  • A strain of Lactobacillus paracasei and its application
  • A strain of Lactobacillus paracasei and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The preparation method of embodiment 1 Lactobacillus paracasei nbk-LC16 bacterium powder

[0067] The culture medium and lyoprotectant used in this embodiment are as follows:

[0068] In terms of mass percentage, the composition of the seed medium is: 3% glucose, 2% peptone, 1.5% yeast extract powder, 1% beef extract, 0.5% anhydrous sodium acetate, 0.05% magnesium sulfate, 0.06% manganese sulfate, 0.1% spit Temperature 80 and the rest of the water, the pH value is 6.8;

[0069]In terms of mass percentage, the composition of the fermentation medium is: 2.3% glucose, 2.5% soybean peptone, 2% yeast extract powder, 0.6% anhydrous sodium acetate, 0.06% sodium alginate, 0.05% magnesium sulfate, 0.03% manganese sulfate, 0.1 % Tween 80 and the balance water.

[0070] In terms of mass percentage, the freeze-drying protective agent includes: 10% trehalose, 8% skimmed milk powder, 0.5% glycerin and the balance of water.

[0071] 1) Lactobacillus paracasei nbk-LC16 was inoculate...

Embodiment 2

[0078] Example 2 Acid-resistant and bile-salt-resistant characteristic research of Lactobacillus paracasei nbk-LC16

[0079] (1) the Lactobacillus paracasei nbk-LC16 bacterium powder that embodiment 1 obtains is respectively inoculated with the inoculum size of 1% (w / v) in the MRS substratum that pH is 6.0 and pH is 37 DEG C and cultivates 3h after adopting Viable bacteria were counted by plate counting method, and the results are shown in Table 1.

[0080] Table 1 Research on acid resistance of Lactobacillus paracasei nbk-LC16

[0081]

[0082] It can be seen that under the condition of pH 3.0, Lactobacillus paracasei nbk-LC16 still has a sufficient number of viable bacteria to pass through the gastric environment after 3 hours.

[0083] (2) the Lactobacillus paracasei nbk-LC16 bacterium powder that embodiment 1 obtains is connected with the inoculum size of 1% (w / v) respectively and be that 0% (w / v) and bile salt concentration are 0.3% The viable bacteria were counted b...

Embodiment 3

[0092] Example 3 Lactobacillus paracasei nbk-LC16 hypoglycemic research in vitro

[0093] The tertiary seed liquid of Lactobacillus paracasei nbk-LC16 was centrifuged for 10 min at a speed of 4 000 r / min (4°C), the supernatant was poured off to get the precipitate, and the PBS solution (concentration 0.1 mol / L, pH = 6.8) Resuspend after washing 2 to 3 times, uniformly adjust the density of the bacterial suspension to 1×10 9 CFU / mL, to prepare bacterial suspension. The determination method is as follows:

[0094] Determination of α-glucosidase inhibition rate: In a system with a total volume of 205 μL, add 50 μL of PBS solution (concentration 0.1mol / L, pH=6.8), and then add 50 μL of p-nitrophenol-α with a concentration of 20 mmol / L -D-glucopyranoside (PNPG) solution and 25 μL of the sample to be tested, incubate the mixture at 37°C for 10 minutes, add 30 μL of α-glucosidase solution 20 U / mL to continue the reaction for 20 minutes, add 50 μL of 1mol / L Na 2 CO 3 As the reacti...

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Abstract

The invention belongs to the technical field of microbes, and in particular relates to a strain of Lactobacillus paracasei with functions of lowering blood sugar, protecting oral cavity and antidepressant and application thereof. The Lactobacillus paracasei nbk-LC16 provided by the invention has strong acid and bile salt resistance, good tolerance in artificial gastric juice and artificial intestinal juice, and can effectively reduce blood sugar and inhibit mutans streptococcus. To study its effect on hyperglycemia mice, after intragastric administration of Lactobacillus paracasei nbk‑LC16 8W, compared with model group mice, the weight of hyperglycemia mice increased significantly, liver index and spleen index decreased significantly, and FBG and PBG significantly decreased. Decreased, HbA1c and insulin levels decreased significantly, liver glycogen content increased. The test results show that Lactobacillus paracasei nbk‑LC16 can regulate blood sugar by regulating insulin levels and HbA1c, and promoting the conversion of blood sugar into liver glycogen. Study its inhibitory effect on Streptococcus mutans, the results of in vitro test and oral cavity test show that it can effectively inhibit Streptococcus mutans, help to protect the health of oral cavity and teeth; at the same time, it has antidepressant effect.

Description

Technical field: [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Lactobacillus paracasei with functions of lowering blood sugar, protecting oral cavity and antidepressant and application thereof. Background technique: [0002] Diabetes is a chronic disease in which the relative or absolute deficiency of insulin secretion in the body leads to metabolic disorders in the body. It is recognized as the third largest chronic disease in the world. Diabetic patients are often accompanied by multiple complications, such as retinopathy, renal arteriosclerosis, and cardiovascular and cerebrovascular diseases, which seriously threaten people's health and even life. With the continuous improvement of people's living standards, the incidence of diabetes is also increasing rapidly. According to the survey results of the International Diabetes Federation, the number of people with diabetes in the world was 240 million in 2007, and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A23K10/18A61K35/747A61P3/10A61P1/02A61P31/04A61P25/24C12R1/225
CPCC12N1/20A23L33/135A23K10/18A61K35/747A61P3/10A61P1/02A61P31/04A61P25/24A23V2002/00C12R2001/225C12N1/205A23V2400/165A23V2200/328A23V2200/312A23V2200/322
Inventor 张阳湛剑龙张丽姣
Owner 诺佰克(武汉)生物科技有限公司
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