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Total triterpenoid extract of inonotus obliquus with hypoglycemic function and pharmaceutical preparation thereof

A technology of Inonotus obliquus and pharmaceutical preparations, which are applied in directions such as drug combination, pill delivery, suppository delivery, etc., can solve problems such as undiscovered total triterpenoids, and achieve the effect of reducing fasting blood glucose level and relieving symptoms of diabetes

Inactive Publication Date: 2010-10-06
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no reports have been found so far about obtaining total triterpenoids from Inonotus obliquus and developing hypoglycemic pharmaceutical preparations using ultrasonic methods

Method used

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  • Total triterpenoid extract of inonotus obliquus with hypoglycemic function and pharmaceutical preparation thereof
  • Total triterpenoid extract of inonotus obliquus with hypoglycemic function and pharmaceutical preparation thereof
  • Total triterpenoid extract of inonotus obliquus with hypoglycemic function and pharmaceutical preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) 100g of Inonotus obliquus is crushed and passed through a 30-mesh sieve, soaked in 800mL of ethanol solution with a volume concentration of 70% concentration for 2 hours, then ultrasonically extracted twice, the power of the ultrasonic wave is 150W, 30min each time, and the extracts are combined , the supernatant was separated by centrifugation, and the supernatant was concentrated and dried to obtain 6.462 g of ethanol extract;

[0022] (2) The ethanol extract is dispersed in distilled water with 20 times by weight of the ethanol extract, then vibratingly extracted with petroleum ether 10 times by weight of the ethanol extract for 3 times each for 2 hours, and the extract and the aqueous phase raffinate separation;

[0023] (3) The above-mentioned aqueous phase raffinate was extracted 3 times with n-butanol shaking 10 times the weight of the ethanol extract, 2 hours each time, and the extract was separated, then the extract was combined, concentrated and dried to o...

Embodiment 2

[0026] (1) 100g of Inonotus obliquus is crushed and passed through a 30-mesh sieve, soaked in 80% ethanol aqueous solution with 1000mL volume concentration for 3 hours, then ultrasonically extracted 3 times, the power of the ultrasonic wave is 100W, 2 hours each time, combined extraction liquid, the supernatant was separated by suction filtration, and the supernatant was concentrated and dried to obtain 10.977 g of ethanol extract;

[0027] (2) disperse the ethanol extract in distilled water with 30 times by weight of the ethanol extract, then use 20 times by weight of petroleum ether vibration extraction of the ethanol extract for 4 times, each time for 3 hours, and extract the extract and the water phase material separation;

[0028] (3) The above-mentioned aqueous phase raffinate is extracted 4 times with n-butanol vibration of 20 times the weight of the ethanol extract, each time for 3 hours, and then the extracts are combined, concentrated and dried to obtain 6.304g of th...

Embodiment 3

[0030] (1) 100g of Inonotus obliquus is crushed and passed through a 30-mesh sieve, soaked in 1200mL of ethanol solution with a volume concentration of 95% concentration for 1 hour, then ultrasonically extracted 4 times, the power of the ultrasonic wave is 100W, 1 hour each time, combined extraction liquid, the supernatant was separated by centrifugation, and the supernatant was concentrated and dried to obtain 4.391 g of ethanol extract;

[0031] (2) Disperse the above-mentioned ethanol extract in distilled water with 50 times by weight of the ethanol extract, then use 30 times by weight of petroleum ether vibration extraction of the ethanol extract for 5 times, each time for 4 hours, extract the extract and the water phase Separation of residue;

[0032] (3) The above-mentioned aqueous phase raffinate is extracted 5 times with n-butanol vibration of 30 times the weight of the ethanol extract, 4 hours each time, and then the extracts are combined, concentrated and dried to ob...

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Abstract

The invention discloses a total triterpenoid extract of inonotus obliquus with a hypoglycemic function, which is prepared through the following steps: (1) crushing and sieving inonotus obliquus, adding ethanol aqueous solution for immersion, carrying out ultrasonic extraction, separating the supernatant, and concentrating and drying the supernatant, thereby obtaining ethanol extract; (2) dispersing the ethanol extract into distilled water, oscillating and extracting with petroleum ether, and separating the extract from water-phase residues generated in extraction; and (3) oscillating and extracting the water-phase residues with n-butanol, separating the extract, mixing, concentrating and drying, thereby obtaining the total triterpenoid extract of the inonotus obliquus with the hypoglycemic function, wherein the content of the triterpenoid is 70-300mg / g. the total triterpenoid extract of the inonotus obliquus with the hypoglycemic function provided by the invention can obviously reduce the fasting blood glucose level of mouse with hyperglycemia, can significantly ease the diabetes symptoms of the mouse with hyperglycemia, and has no significant toxicity to major organs.

Description

technical field [0001] The invention belongs to the field of pharmacy, in particular to a total triterpene extract of Inonotus obliquus with hypoglycemic effect and a pharmaceutical preparation thereof. Background technique [0002] Diabetes mellitus is a common endocrine and metabolic disease with a genetic tendency. Its pathophysiological changes are absolute or relative insufficiency of insulin secretion, causing metabolic disorders of sugar, fat, and protein. At present, diabetes has become a common and frequently-occurring disease in many countries around the world, and its mortality rate ranks third after tumors and cardiovascular diseases. More than 90% of diabetic patients in the world suffer from type 2 diabetes, that is, non-insulin-dependent Diabetes mellitus (NID.DM). In addition, the current epidemic trend of diabetes is younger. WHO predicts that by 2025, only 26% of the diabetic population in developing countries will be over 65 years old, while in developed ...

Claims

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Application Information

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IPC IPC(8): A61K36/07A61K9/16A61K9/48A61K9/14A61K9/20A61K9/10A61K9/02A61P3/10A61P3/06
Inventor 陈海霞董鹏邢玥
Owner TIANJIN UNIV
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