Mollugin and preparation method and application thereof

A kind of rubidin and grassland technology, which is applied in the preparation of drugs for treating diabetes and the application of drugs for lowering blood sugar. In the field of rubidin and its preparation, it can solve the pharmacological activity of rubidin. No research report has been found and other problems, to achieve a good hypoglycemic effect, a significant effect, and the effect of lowering blood sugar levels

Inactive Publication Date: 2013-01-16
HENAN UNIVERSITY
View PDF3 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Rubidolin is a compound extracted and isolated from Rubia radiata. At present, it is mainly used for the treatment of pain caused by gastrointestinal spasms, gastroenteritis, intestinal ascariasis, biliary ascariasis, cholelithiasis and other biliary diseases. There is no research report on other pharmacological activities of rubikin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mollugin and preparation method and application thereof
  • Mollugin and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The rubiacin in this example is obtained by extracting and eluting from madder, and the specific preparation method is as follows: take the dried madder medicinal material, heat and reflux with 5 times the amount of petroleum ether for extraction for 40 minutes; recover the petroleum ether, and dry it in vacuum The petroleum ether was removed to obtain the extract; the extract was subjected to silica gel 200-300 mesh column chromatography, eluted with petroleum ether, detected by TLC, combined with components rich in rubidin, and then subjected to Sephadex LH-20 gel column chromatography, using Chloroform:petroleum ether:methanol=5:5:1 for elution, and then through silica gel H column decompression column chromatography, eluting with petroleum ether, and finally recrystallizing with hot ethanol to obtain rubiacin.

Embodiment 2

[0022] The rubiacin in this example is obtained by extracting and eluting from madder, and the specific preparation method is as follows: take the dried madder medicinal material, heat and reflux with 10 times the amount of petroleum ether for extraction for 30 minutes; recover petroleum ether, and vacuum-dry The petroleum ether was removed to obtain the extract; the extract was subjected to silica gel 200-300 mesh column chromatography, eluted with petroleum ether, detected by TLC, combined with components rich in rubidin, and then subjected to Sephadex LH-20 gel column chromatography, using Chloroform:petroleum ether:methanol=5:5:1 for elution, and then through silica gel H column decompression column chromatography, eluting with petroleum ether, and finally recrystallizing with hot ethanol to obtain rubiacin.

Embodiment 3

[0024] The rubiacin in this example is obtained by extracting and eluting from madder. The specific preparation method is as follows: take the dried madder medicinal material, heat and reflux with 15 times the amount of petroleum ether to extract for 20 minutes; recover the petroleum ether, and dry it in vacuum The petroleum ether was removed to obtain the extract; the extract was subjected to silica gel 200-300 mesh column chromatography, eluted with petroleum ether, detected by TLC, combined with components rich in rubidin, and then subjected to Sephadex LH-20 gel column chromatography, using Chloroform:petroleum ether:methanol=5:5:1 for elution, and then through silica gel H column decompression column chromatography, eluting with petroleum ether, and finally recrystallizing with hot ethanol to obtain rubiacin.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of mollugin. The preparation method includes: taking India madder root, and using 5-15 times of petroleum ether to perform heating reflux extraction for 20-40min; recycling petroleum ether, and vacuum drying to remove petroleum ether so as to obtain extract; and subjecting the extract to 200-300-mesh silica gel column chromatography, eluting with petroleum ether, performing TLC (thin layer chromatography) detection, combining components rich in mollugin, subjecting to Sephadex LH-20 gel column chromatography, eluting with mixture of chloroform, petroleum ether, and methanol in mass ratio of 5:5:1, subjecting to silica gel H-column decompression column chromatography, eluting with petroleum ether, and recrystalizing with hot ethanol to obtain the mollugin. The invention further discloses application of the mollugin in preparation of hypoglycemic drugs and preparation of drugs for treating diabetes. Experiments show that the mollugin can effectively reduce blood sugar, TC (total cholesterol), TG (total glycerin) and MDA (methylene dioxyamphetamine) content of mice with high blood sugar caused by alloxan, and can evidently increase SOD (superoxide dismutase) activity and hepatic glycogen content of the mice.

Description

technical field [0001] The present invention relates to a rubidin and a preparation method thereof, and also relates to its application in the preparation of hypoglycemic drugs and the preparation of diabetes treatment drugs, and belongs to the field of natural medicines. Background technique [0002] Diabetes (diabetes) is a sugar, protein, Syndrome of a series of metabolic disorders including fat, water and electrolytes. There are two main types of diabetes: type 1 diabetes (insulin-dependent diabetes) and type 2 diabetes (non-insulin-dependent diabetes). Clinically, hyperglycemia is the main feature. In typical cases, symptoms such as polyuria, polydipsia, polyphagia, and emaciation may appear, that is, symptoms of "three excesses and one deficiency". Once diabetes (blood sugar) is not well controlled, it will cause complications, leading to failure of the kidneys, eyes, feet and other parts, and it cannot be cured. Foreign countries give it an alias "Silent Killer" (S...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/92A61K31/352A61P3/10A61P3/06A61P39/06
Inventor 康文艺张伟
Owner HENAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap