Bovine parainfluenza virus PBIV3-B strain and application thereof

A bovine parainfluenza virus, PBIV3-B technology, applied in the direction of viruses, antiviral agents, virus antigen components, etc., can solve the problem of no bovine parainfluenza virus vaccine

Active Publication Date: 2017-11-10
INST OF ANIMAL SCI CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no commercial bovine parainfluenza virus vaccine and detection reagents in China

Method used

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  • Bovine parainfluenza virus PBIV3-B strain and application thereof
  • Bovine parainfluenza virus PBIV3-B strain and application thereof
  • Bovine parainfluenza virus PBIV3-B strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation and culture identification of bovine parainfluenza virus PBIV3-B strain

[0024] 1 Source and standard of virus species

[0025] According to the new biological product declaration requirements, combined with the large amount of experimental data obtained in the present invention, with reference to the "Chinese Veterinary Pharmacopoeia" (2005 edition), the seedlings used in the production of poisons have been identified. The trial regulations (draft) poisoning seed standards are now as follows Description.

[0026] 1.1 Source of the virus

[0027] The virus species used in the manufacture of this product is the bovine parainfluenza virus PBIV3-B strain, which was obtained by the inventors after self-isolation and domestication from diseased cattle in 2015. Because this strain is derived from a typical bovine parainfluenza virus type 3 infection in cattle It is isolated from the virus and has a high proliferation titer on the cell compared with other isolate...

Embodiment 2

[0056] Example 2 Preparation of inactivated vaccine

[0057] The preparation process of the bovine parainfluenza virus PBIV3-B strain is prepared according to the currently mature commercial vaccine preparation process. Judging from the quality inspection results of 3 batches of vaccines prepared in the laboratory and 5 batches of vaccines in the intermediate trial production, the quality of the vaccine products prepared by this process is stable, and the results of the efficacy inspections fully meet the established quality standards.

[0058] 1 Preparation of virus liquid

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Abstract

The present invention discloses bovine parainfluenza virus PBIV3-B strain and application thereof, the bovine parainfluenza virus PBIV3-B strain has the advantages of good immunogenicity and culture characteristics and the like, the bovine parainfluenza virus PBIV3-B strain is inoculated into MDBK cell to obtain a culture matter, after the culture matter is inactivated with (beta-Propiolactone), the culture matter is mixed with commercial MontanideTMISA15AVG adjuvant of SEPPIC of France for emulsion to obtain a bovine parainfluenza virus inactivated vaccine. Results showed that when the bovine parainfluenza virus inactivated vaccine prepared by the method is used for immunization of a weaned healthy negative cow, diseases caused by the bovine parainfluenza virus type 3 can be prevented, and the bovine parainfluenza virus inactivated vaccine has the advantages of safety, fast antibody production and lasting immunity period and the like.

Description

Technical field [0001] The present invention belongs to the field of biology, and more specifically, belongs to the field of biological vaccines. It relates to a bovine infectious rhinotracheitis virus and its application, and particularly relates to a self-isolated bovine parainfluenza virus PBIV3-B strain and a strain derived from the virus. The prepared inactivated vaccine. Background technique [0002] Bovine parainfluenza is an acute and contagious infectious disease caused by Bovine parainfluenza virus 3 (PBIV3), also known as "transport fever". PBIV3 belongs to the Paramyxoviridae family and the genus Paramyxovirus. It is a single-stranded negative-stranded RNA virus that mainly causes acute respiratory diseases in cattle and other ruminants. In 1959, Reisinger et al. first isolated PBIV3 from calf kidneys in the United States. Since then, countries such as France, the former Soviet Union, Japan, Denmark, Canada, Australia, Panama, and Italy have also successively isolat...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/155A61P31/14C12R1/93
CPCA61K39/12A61K2039/5252C12N7/00C12N2760/18621C12N2760/18634
Inventor 崔尚金赵占中梁琳
Owner INST OF ANIMAL SCI CAAS
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