Primer and probe for detecting echinococcus granulosus in canine feces, and kit of primer and probe

A technology of Echinococcus granulosus and a kit, which is applied in the field of bioengineering, can solve the problems of manpower consumption and complicated process, and achieve the effects of high efficiency and accuracy, controllable cost and obvious technological progress

Inactive Publication Date: 2017-11-21
STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned technical problems in the prior art, the present invention provides a primer, a probe and a kit thereof for detecting Echinococcus granulosus in dog feces. The primers, probes and kits for Taenia coccus should solve the technical problems of complex, labor-intensive and time-consuming process of detecting Echinococcus granulosus in dog feces in the prior art

Method used

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  • Primer and probe for detecting echinococcus granulosus in canine feces, and kit of primer and probe
  • Primer and probe for detecting echinococcus granulosus in canine feces, and kit of primer and probe
  • Primer and probe for detecting echinococcus granulosus in canine feces, and kit of primer and probe

Examples

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Effect test

Embodiment 1

[0020] Example 1 Obtaining specific primers and probes for real-time fluorescence quantitative PCR primers for distinguishing Echinococcus granulosus in dog feces.

[0021] According to the mitochondrial cytochrome c oxidase subunit I gene cox1 of Echinococcus granulosus (GenBank TM accession number: MF004310), using Primer Express 3.0 software to design and synthesize Taqman fluorescent quantitative PCR specific primers and probes, including:

[0022] A group of real-time fluorescent quantitative PCR primers and probes for specifically amplifying Echinococcus granulosus, the nucleotide sequence of which is:

[0023] Upstream primer EgF: 5'-GTTGGTTTTTTTTGTTGGTTAGTATGTG-3',

[0024] Downstream primer EgR: 5'-ACTAAAAACACTTGAAACACCAGCC-3',

[0025] Probe EG: 5'-F-ATTTTATCCGCCGTTGTCCTCGTCG-Q-3'.

[0026] Among them, F is the fluorescent reporting group ROX, and Q is the fluorescent quenching group TAMRA.

[0027] The amplification system used in the real-time quantitative PCR ...

Embodiment 2

[0034] Example 2 Detection Application of Echinococcus granulosus Eggs in Dog Feces

[0035] According to the method of Example 1, 30 samples of dog feces from Tibet were tested by fluorescent quantitative PCR, and then the gold standard of etiology and genetic testing - gene sequencing method was used to sequence and test the 30 samples. The results showed that the results of Taqman fluorescent quantitative PCR experiment were 100% consistent with the results of etiology and gene sequencing.

[0036]

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Abstract

The invention provides a primer for detecting echinococcus granulosus in canine feces, wherein the sequence of an upstream primer is as shown in SEQ ID No. 1; and the sequence of a downstream primer is as shown in SEQ ID No. 2. The invention also provides a probe for detecting the echinococcus granulosus in the canine feces, wherein the sequence is as shown in SEQ ID No. 3. The invention further provides a kit for detecting the echinococcus granulosus in the canine feces; the kit comprises the primer for detecting the echinococcus granulosus in the canine feces and the probe for detecting the echinococcus granulosus in the canine feces, wherein the sequence of the upstream primer is as shown in SEQ ID No. 1; the sequence of the downstream primer is as shown in SEQ ID No. 2; and the sequence of the probe is as shown in SEQ ID No. 3. In the invention, by using the primer, the probe and the kit, a target gene DNA can be rapidly and specifically detected with high sensitivity; even when a specimen contains the trace amount of echinococcus DNA, the target gene DNA can be detected.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a method for detecting parasites, in particular to a primer, a probe and a kit for detecting Echinococcus granulosus in dog feces. Background technique [0002] Echinococcus granulosus (Echinococcus granulosus) is a tapeworm of the genus Echinococcus, also known as the tapeworm. Adults parasitize canids, and their larvae, echinococcosis, can parasitize a variety of ruminants and humans, causing a serious zoonotic disease called echinococcosis or echinococcosis ( hydatid disease, hydatidosis). The disease is distributed worldwide and is mainly prevalent in Europe, northern Africa, Asia, South America and Oceania. In my country, it is mainly found in grazing areas such as Xinjiang, Ningxia, Qinghai, Tibet, Gansu and Aba in Sichuan. The final host of echinococcosis is mainly canine animals. The diagnosis of Echinococcus infection mainly includes fecal egg examination, autopsy, arecolin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2561/101C12Q2563/107C12Q2561/113
Inventor 陈家旭肖宁陈韶红艾琳陈木新田利光储言红卢艳蔡玉春吴秀萍俞英昉宋鹏陈海宁李浩
Owner STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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