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Cell preservation solution and application thereof

A technology for preservation of liquid and cells, applied in the field of medical testing, can solve the problems of high price of cell preservation solution, unsatisfactory treatment of sample impurities, and short storage time of cells, so as to facilitate medical detection and analysis, improve the effect of impurity treatment, and reduce interference factors. Effect

Pending Publication Date: 2017-12-01
GUANGZHOU HONGQI OPTICAL INSTR TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The current cell preservation solutions generally have the defects of high price and short storage time of cells, especially for the processing of clinical cytology samples with a lot of blood or mucus, the treatment of specimen impurities is not ideal, and interfering cells such as blood cells and mucus are prepared. The factor treatment is also not satisfactory

Method used

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  • Cell preservation solution and application thereof
  • Cell preservation solution and application thereof
  • Cell preservation solution and application thereof

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Experimental program
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Effect test

Embodiment 1

[0038] Three kinds of cell preservation solutions in this embodiment include the following components (Table 1):

[0039] Table 1

[0040]

[0041] Mix the components according to the dosage in Table 1 to obtain the cell preservation solution.

Embodiment 2

[0043] Three kinds of cell preservation solutions in this embodiment include the following components (Table 2):

[0044] Table 2

[0045]

[0046]

[0047] Mix the components according to the dosage in Table 2 to obtain the cell preservation solution.

Embodiment 3

[0049] Four kinds of cell preservation solutions of the present embodiment include the following components (Table 3):

[0050] table 3

[0051]

[0052]

[0053] Mix the components according to the dosage in Table 3 to obtain the cell preservation solution.

[0054] The leucorrhea / cervical exfoliated cells of the same batch were treated with the above-mentioned cell preservation solutions, sliced ​​with HQ TCT automatic film production machine, stained with HQ Papanicolaou staining solution, and sealed for observation. The results are shown in Table 4.

[0055] Table 4

[0056]

[0057]The longest storage time: After sampling, the cell sample is added with preservation solution, which can be stored for 2 months before testing. Generally, the existing cell preservation solution can only be stored for 3 weeks. Once it exceeds 1 month, the sample can no longer be used.

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Abstract

The invention provides a cell preservation solution and application thereof. The cell preservation solution is prepared from the following components in parts by weight: 60 to 1000 parts of pH buffer, 10 to 150 parts of osmotic pressure maintenance agent, 4 to 55 parts of anticoagulant, 1 to 30 parts of mucolytic agent, 800 to 5000 parts of fixing agent, 1 to 150 parts of component A, 10 to 30 parts of component B and 10000 to 20000 parts of water, wherein the component A is prepared from sodium azide and lauryl sodium sulfate according to a weight ratio of (1 to 10) to (2 to 110); the component B is carbon disulfide. The cell preservation solution of the invention has a better impurity treatment effect, and reduces the interference factors in cell samples; at the same time, target cells therein can retain the characteristics of structural morphology for a long time, thereby facilitating the medical detection and analysis of the target cells, and the cost is low.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a cell preservation solution and its application. Background technique [0002] As a screening method for cervical lesions, many factors involved in the traditional Pap smear process, such as sampling, smear quality, and cell separation, will seriously reduce the positive rate of detection. Cytology experts have introduced a diagnostic method for cell preparation - liquid-based thin-layer cytology test (Thinprep Cytology Test; TCT for short), which mainly discards non-diagnostic impurities through technical processing, and produces thin-layer cells that are easy to observe and clear The film makes it easier for the reader to observe, and the diagnostic accuracy is significantly improved. [0003] TCT technology can maximize the preservation of meaningful cells, eliminate artificial loss and destruction, fix the sample cells in the solution, that is, terminate any ongoing biochem...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0205
Inventor 范静彦刘忠贵李必松罗琪
Owner GUANGZHOU HONGQI OPTICAL INSTR TECH
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