A kind of polypeptide hip-13 that can antagonize the rna binding activity of hur protein and its application

A technology that combines activity and protein, and is applied in the direction of polypeptides, hybrid peptides, peptide/protein components containing positioning/targeting motifs, etc., which can solve the problems of large side effects, high cancer recurrence rate and death risk, and achieve tumor inhibition. Effect

Active Publication Date: 2020-03-27
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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Problems solved by technology

Although in the past few decades, due to the advancement of surgery, radiotherapy and chemotherapy, tumor treatment has made great progress, but these methods often have severe side effects during treatment, and most cancers still have a high recurrence rate and death risk

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  • A kind of polypeptide hip-13 that can antagonize the rna binding activity of hur protein and its application
  • A kind of polypeptide hip-13 that can antagonize the rna binding activity of hur protein and its application
  • A kind of polypeptide hip-13 that can antagonize the rna binding activity of hur protein and its application

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Embodiment Construction

[0025] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention.

[0026] 1. Synthesis of Antitumor Peptides

[0027] Synthesize an anti-tumor polypeptide by solid-phase synthesis, which includes a tumor cell killing domain and a membrane-penetrating domain, wherein the tumor cell killing domain sequence is shown in SEQ ID NO: 1, and the membrane-penetrating domain sequence is as shown in SEQ ID NO: 1 As shown in ID NO: 2, it is connected to the N-terminal of the tumor cell killing domain, and the obtained sequence is: the amino acid sequence is YGRKKRRQRRR-VAGHSLGYGFVNK (SEQ ID NO: 3), named HIP-13. For the convenience of research, we linked FITC labeled with fluorescein isothiocyanate to the C-terminus of the anti-tumor polypeptide, and linked biotin-labeled Biotin to the N-terminus. It was synthesized by Shanghai...

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Abstract

The present invention provides a polypeptide that can antagonize the RNA binding activity of HuR protein and its application, and its amino acid sequence is shown in SEQ ID NO: 1; it also relates to an anti-tumor polypeptide and its application, and the anti-tumor polypeptide includes tumor cells The killing domain and the membrane-penetrating domain, the amino acid sequence of the tumor cell killing domain is shown in SEQ ID NO:1. The transmembrane domain of the anti-tumor polypeptide of the present invention has no cytotoxicity itself, but after linking with the tumor cell killing domain, it has obvious effects of inhibiting tumor proliferation, migration and invasion. The anti-tumor polypeptide of the present invention can not only be used as an anti-tumor biotherapeutic drug alone, but is also expected to be combined with other treatment methods to inhibit tumors.

Description

technical field [0001] The present invention relates to the field of tumor targeting therapy, and more particularly relates to a polypeptide capable of antagonizing the RNA binding activity of HuR protein and its application. Background technique [0002] Cancer is the main cause of human death and a major disease that endangers human health. According to the International Agency for Research on Cancer, there were 14.1 million new cases and 8.2 million deaths worldwide in 2012. Although in the past few decades, due to the advancement of surgery, radiotherapy and chemotherapy, tumor treatment has made great progress, but these methods often have severe side effects during treatment, and most cancers still have a high recurrence rate and death risk. Therefore, it is very necessary and urgent to seek new treatment measures to improve the survival rate and cure rate of cancer. [0003] Molecular targeted therapy, which has emerged in recent years, aims at the molecular level ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C07K19/00A61K38/10A61K47/42A61P35/00
CPCA61K38/00C07K7/08C07K2319/10
Inventor 郑丽端童强松陈亚俊杨枫李欢欢叶霖李聃宋华杰
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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