Treatment method for obtaining dendrobium polysaccharide from dendrobium by alcohol precipitation method

Abstract

The invention relates to the technical field of the deep processing of dendrobium, and particularly relates to a treatment method for obtaining a dendrobium polysaccharide from dendrobium by an alcohol precipitation method. The treatment method comprises the following specific steps of placing a fresh stem of the dendrobium in saline water, mechanically agitating, leaching the treated dendrobium twice by adopting a soluble iron ion aqueous solution at 80 DEG C according to a solid-liquid ratio of 1 to 30, adding absolute ethyl alcohol into leaching liquor, enabling the concentration of the ethyl alcohol in a solution to reach 65 percent, regulating the pH (potential of Hydrogen) of the solution to 8.2 to 8.5, standing, thus obtaining a precipitate, centrifuging, collecting supernatant liquor and freeze-drying the supernatant liquor and the precipitate. Through the design of the treatment method, the treatment method is used for obtaining the dendrobium polysaccharide from the dendrobium by the alcohol precipitation method; not only is the yield of the dendrobium polysaccharide high, but also the contents of active material components such as an alkaloid, polyphenol and flavone in the generated supernatant liquor are also improved obviously.

Description

technical field [0001] The invention relates to the technical field of dendrobium deep processing, in particular to a treatment method for obtaining dendrobium polysaccharides from dendrobium by an alcohol precipitation method. Background technique [0002] Dendrobium, a monocotyledonous plant of the Orchidaceae Dendrobium genus, also known as Immortal Grass and Resurrection Grass, is the first of the "Nine Immortal Herbs" in my country. It has a long history of medicinal use. Stomach-promoting, liver-protecting, gallbladder-protecting, and skin-moisturizing effects. The flowers of Dendrobium are gorgeous in color and can be used for viewing. The stem of Dendrobium is the main source of biological activity. In recent years, studies have shown that Dendrobium contains alkaloids, polyphenols, flavonoids and reducing sugars. and other substances have biological activities such as anti-oxidation, improving immunity and anti-inflammation. Dendrobium polysaccharides are the most imp...

AI Technical Summary

Problems solved by technology

[0002] Dendrobium, a monocotyledonous plant of the Orchidaceae Dendrobium genus, also known as Immortal Grass and Resurrection Grass, is the first of the "Nine Immortal Herbs" in my country. It has a long history of medicinal use. Stomach-promoting, liver-protecting, gallbladder-protecting, and skin-moisturizing effects. The flowers of Dendrobium are gorgeous in color and can be used for viewing. The stem of Dendrobium is the main source of biological activity. In recent years, studies have shown that Dendrobium contains alkaloids, polyphenols, flavonoids and reducing sugars. and other substances have biological activities such as anti-oxidation, improving immunity and anti-inflammation. Dendrobium polysaccharides are the most important active substances of Dendrobium, and their pharmacological effects have attracted widespread attention. Leaching, water extraction and alcohol precipitation, etc. In actual production, a large amount of supernatant is produced in the process of alcohol precipitation of polysaccharides, which contains rich active substances, mainly alkaloids, polyphenols, and flavonoids. The extraction process of dendrobium polysaccharides obtained by alcohol precipitation is mainly to increase the yield of dendrobium polysaccharides, and there are few studies on how to increase the content of active substances in the supernatant