Treatment method for obtaining dendrobium polysaccharide from dendrobium by alcohol precipitation method

A kind of dendrobium polysaccharide, processing method technology, applied in the field of processing to obtain dendrobium polysaccharide, can solve the problem of less research and the like

Inactive Publication Date: 2017-12-15
霍山县长冲中药材开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Dendrobium, a monocotyledonous plant of the Orchidaceae Dendrobium genus, also known as Immortal Grass and Resurrection Grass, is the first of the "Nine Immortal Herbs" in my country. It has a long history of medicinal use. Stomach-promoting, liver-protecting, gallbladder-protecting, and skin-moisturizing effects. The flowers of Dendrobium are gorgeous in color and can be used for viewing. The stem of Dendrobium is the main source of biological activity. In recent years, studies have shown that Dendrobium contains alkaloids, polyphenols, flavonoids and reducing sugars. and other substances have biological activities such as anti-oxidation, improving immunity and anti-inflammation. Dendrobium polysaccharides are the most important active substances of Dendrobium, and their pharmacological effects have attracted widespread attention. Leaching, water extraction and alcohol precipitation, etc. In actual production, a large amount of supernatant is produced in the process of alcohol precipitation of polysaccharides, which contains rich active substances, mainly alkaloids, polyphenols, and flavonoids. The extraction process of dendrobium polysaccharides obtained by alcohol precipitation is mainly to increase the yield of dendrobium polysaccharides, and there are few studies on how to increase the content of active substances in the supernatant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, a kind of processing method that obtains dendrobium polysaccharide from dendrobium with alcohol precipitation method, concrete steps are as follows:

[0017] Place the fresh stem of dendrobium at 5°C and mechanically stir in salt water with a concentration of 7% by weight, the volume ratio of the fresh stem of dendrobium to the salt water is 1:5, the mechanical stirring speed is 20r / min, and the stirring time is 20 minutes;

[0018] The treated dendrobium was extracted twice with 80° C. soluble iron ion aqueous solution according to the solid-liquid ratio of 1:30, each time for 2 hours, centrifuged, and the leaching solution was combined. The concentration of the soluble iron ion aqueous solution was 0.03mol / L;

[0019] Add absolute ethanol to the extract solution to make the ethanol concentration in the solution reach 65%, adjust the pH of the solution to 8.2, let stand to obtain a precipitate, centrifuge to collect the supernatant, freeze-dry the supernat...

Embodiment 2

[0020] Embodiment 2, a kind of processing method that obtains dendrobium polysaccharide from dendrobium with alcohol precipitation method, concrete steps are as follows:

[0021] The fresh stems of Dendrobium were placed at 6°C and mechanically stirred in salt water with a concentration of 7.5% by weight, the volume ratio of the fresh stems of Dendrobium to salt water was 1:5.3, the mechanical stirring speed was 22r / min, and the stirring time was 22 minutes;

[0022] The treated dendrobium was extracted twice with 80°C soluble iron ion aqueous solution at a ratio of 1:30 to liquid, for 2 hours each time, centrifuged, and the extracts were combined. The concentration of the soluble iron ion aqueous solution was 0.038mol / L;

[0023] Add absolute ethanol to the extract solution to make the ethanol concentration in the solution reach 65%, adjust the pH of the solution to 8.3, stand still to obtain a precipitate, centrifuge to collect the supernatant, freeze-dry the supernatant and ...

Embodiment 3

[0024] Embodiment 3, a kind of processing method that obtains dendrobium polysaccharide from dendrobium with alcohol precipitation method, concrete steps are as follows:

[0025] The fresh stems of Dendrobium were placed at 7°C and mechanically stirred in salt water with a concentration of 8.5% by weight, the volume ratio of the fresh stems of Dendrobium to salt water was 1:5.7, the mechanical stirring speed was 26r / min, and the stirring time was 24 minutes;

[0026] The treated dendrobium was extracted twice with 80°C soluble iron ion aqueous solution at a ratio of 1:30 to liquid, for 2 hours each time, centrifuged, and the extracts were combined. The concentration of the soluble iron ion aqueous solution was 0.045mol / L;

[0027] Add absolute ethanol to the extract solution to make the ethanol concentration in the solution reach 65%, adjust the pH of the solution to 8.4, let stand to obtain a precipitate, centrifuge to collect the supernatant, freeze-dry the supernatant and th...

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Abstract

The invention relates to the technical field of the deep processing of dendrobium, and particularly relates to a treatment method for obtaining a dendrobium polysaccharide from dendrobium by an alcohol precipitation method. The treatment method comprises the following specific steps of placing a fresh stem of the dendrobium in saline water, mechanically agitating, leaching the treated dendrobium twice by adopting a soluble iron ion aqueous solution at 80 DEG C according to a solid-liquid ratio of 1 to 30, adding absolute ethyl alcohol into leaching liquor, enabling the concentration of the ethyl alcohol in a solution to reach 65 percent, regulating the pH (potential of Hydrogen) of the solution to 8.2 to 8.5, standing, thus obtaining a precipitate, centrifuging, collecting supernatant liquor and freeze-drying the supernatant liquor and the precipitate. Through the design of the treatment method, the treatment method is used for obtaining the dendrobium polysaccharide from the dendrobium by the alcohol precipitation method; not only is the yield of the dendrobium polysaccharide high, but also the contents of active material components such as an alkaloid, polyphenol and flavone in the generated supernatant liquor are also improved obviously.

Description

technical field [0001] The invention relates to the technical field of dendrobium deep processing, in particular to a treatment method for obtaining dendrobium polysaccharides from dendrobium by an alcohol precipitation method. Background technique [0002] Dendrobium, a monocotyledonous plant of the Orchidaceae Dendrobium genus, also known as Immortal Grass and Resurrection Grass, is the first of the "Nine Immortal Herbs" in my country. It has a long history of medicinal use. Stomach-promoting, liver-protecting, gallbladder-protecting, and skin-moisturizing effects. The flowers of Dendrobium are gorgeous in color and can be used for viewing. The stem of Dendrobium is the main source of biological activity. In recent years, studies have shown that Dendrobium contains alkaloids, polyphenols, flavonoids and reducing sugars. and other substances have biological activities such as anti-oxidation, improving immunity and anti-inflammation. Dendrobium polysaccharides are the most imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 何祥林
Owner 霍山县长冲中药材开发有限公司
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