A pre-assessment method for enzymatic hydrolysis of lignocellulose to produce sugar
A lignocellulosic enzyme and lignocellulose technology, applied in biochemical equipment and methods, measurement/inspection of microorganisms, weighing by removing certain components, etc., can solve the problems of reducing enzymatic hydrolysis efficiency and avoid low Efficient enzymatic hydrolysis, wide application, significant practical effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0037] The lignin content of sample 1 (corn stalk) was determined to be 0.235, with a standard deviation of 0.003; the cellulose content was 0.500, with a standard deviation of 0.004, and the calculated glycation index was 0.01225. Take 0.5g of sample 1 for enzymatic hydrolysis experiment (add 20mL of 0.5mM citric acid buffer (pH=4.8) and 30μl of Novozymes (Finland) CTec2 cellulase for hydrolysis at 50°C, 120rpm for 72 hours), followed by DNS method The yield of reducing sugar was determined. After three repeated experiments, the yield of reducing sugar was measured to be 261.11 mg / g, with a standard deviation of 4.332. It can be seen from Table 2 that the saccharification index of 0.01225<0.250 belongs to the low-potential area, the reducing sugar yield is predicted to be less than 400mg / g, and the actual sugar yield is 261.11mg / g, which proves that the prediction is accurate. The potential sugar yield of this sample is low and is not suitable for practical applications.
Embodiment 2
[0039] The lignin content of sample 2 (corn stalk) was determined to be 0.125, and the standard deviation was 0.005; the cellulose content was 0.485, and the standard deviation was 0.004. The calculated glycation index was 0.2847. The reducing sugar determination process was the same as that of Example 1. After three repeated tests , the yield of reducing sugar was measured to be 430.76 mg / g, with a standard deviation of 8.182. It can be seen from Table 2 that the saccharification index of 0.650>0.2847>0.250 belongs to the middle potential area, the predicted value of reducing sugar production is 400-800 mg / g, and the actual sugar production is 430.76 mg / g, which proves that the prediction is accurate, and the potential sugar production of this sample is moderate.
Embodiment 3
[0041] The lignin content of sample 3 (corn stalk) was determined to be 0.015, with a standard deviation of 0.001; the cellulose content was 0.728, with a standard deviation of 0.008, and the calculated glycation index was 0.88481. The reducing sugar determination process was the same as in Example 1, and the test was repeated three times. , the yield of reducing sugar was measured to be 924.56 mg / g with a standard deviation of 7.541. It can be seen from Table 2 that the glycation index of 0.88481>0.650 belongs to the high potential area, the predicted value of reducing sugar production is >800mg / g, and the actual sugar production is 924.56mg / g, which proves that the prediction is accurate, and the potential sugar production of this sample is high.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More