Pre-estimate method of enzymolysis and sugar producing abilities of lignocellulose
A lignocellulase, lignocellulose technology, applied in biochemical equipment and methods, microbial determination/inspection, weighing by removing certain components, etc., can solve problems such as reducing the efficiency of enzymatic hydrolysis, and avoid low Efficient enzymatic hydrolysis, simple equipment and strong reliability
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Embodiment 1
[0037] Sample 1 (corn stalk) was measured to have a lignin content of 0.235 with a standard deviation of 0.003; a cellulose content of 0.500 with a standard deviation of 0.004, and a calculated glycation index of 0.01225. Take 0.5g of sample 1 for enzymatic hydrolysis (add 20mL of 0.5mM citrate buffer (pH=4.8) and 30μl of Novozymes (Finland) CTec2 cellulase at 50°C, 120rpm for 72 hours), and then use the DNS method Determination of the reducing sugar yield, through three repeated tests, the measured reducing sugar yield was 261.11mg / g, and the standard deviation was 4.332. It can be seen from Table 2 that the glycation index of 0.01225<0.250 belongs to the low potential area, the reducing sugar production is predicted to be <400mg / g, and the actual sugar production is 261.11mg / g, which proves that the prediction is accurate, and the potential sugar production of this sample is low, which is not suitable for practical application.
Embodiment 2
[0039] Sample 2 (corn stalks) was measured to obtain a lignin content of 0.125 with a standard deviation of 0.005; a cellulose content of 0.485 with a standard deviation of 0.004; the calculated glycation index was 0.2847, and the reducing sugar measurement process was the same as that of Example 1, after three repeated tests , the measured reducing sugar yield was 430.76mg / g, and the standard deviation was 8.182. It can be seen from Table 2 that the glycation index 0.650>0.2847>0.250 belongs to the medium potential area, the predicted value of reducing sugar production is 400-800 mg / g, and the actual sugar production is 430.76 mg / g, which proves that the prediction is accurate and the potential sugar production of this sample is moderate.
Embodiment 3
[0041] Sample 3 (corn stalks) was measured to obtain a lignin content of 0.015 with a standard deviation of 0.001; a cellulose content of 0.728 with a standard deviation of 0.008; the calculated glycation index was 0.88481; , the measured reducing sugar yield was 924.56mg / g, and the standard deviation was 7.541. It can be seen from Table 2 that the glycation index of 0.88481>0.650 belongs to the high potential area, the predicted value of reducing sugar production is >800mg / g, and the actual sugar production is 924.56mg / g, which proves that the prediction is accurate and the potential sugar production of this sample is high.
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