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Kit for determining thrombin time (TT)

A technology of thrombin time and reagent kit, which is applied in the field of medical testing, can solve the problems of large reagent deviation and inconvenient use, and achieve the effects of avoiding differences between bottles, avoiding large differences, and saving dosage

Inactive Publication Date: 2018-01-09
山东艾科达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of large deviation and inconvenient use of current reagents for measuring thrombin time, the present invention provides a kit for measuring thrombin time with reliable results and stable reagents

Method used

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  • Kit for determining thrombin time (TT)
  • Kit for determining thrombin time (TT)
  • Kit for determining thrombin time (TT)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Preparation of kit for measuring thrombin time.

[0021] Dextran 20, polyethylene glycol 6000, Triton X-100, bovine thrombin, bovine serum albumin and ProClin 300 were weighed and dissolved in 20mM Tris-HCl buffer at pH 7.8 in turn, so that the respective concentrations were 3 %, 0.1%, 0.3%, 5 IU / mL, 1% and 0.02%, stir until completely dissolved and uniform.

Embodiment 2

[0022] Example 2 Determination of thrombin time (TT).

[0023] Adopt the kit of embodiment 1 and commercially available reagent to measure the same plasma sample with automatic hemagglutination instrument, the main parameter settings of the instrument are shown in Table 1; record the thrombin time, the repeatability comparison results of reagent of the present invention and commercially available reagent are shown in Table 1. Table 2. Table 2 shows that compared with commercially available reagents, the reagents of the present invention have smaller coefficient of variation (CV) values ​​and better repeatability.

[0024] The reagent of Example 1 and the commercially available reagent are opened simultaneously, and the commercially available reagent is used after reconstitution of freeze-dried powder, and the reagent of the present invention is directly used, and a quality control test is performed on the same coagulation instrument, and the date and time after opening the bot...

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Abstract

The invention provides a kit for determining thrombin time (TT). The kit comprises a thrombin preparation, a preservative, a stabilizer and a buffer solution. The stabilizer is a mixture of polyethylene glycol 6000, bovine serum albumin, Triton X-100 and dextranum, the concentrations of which in the kit are separately 0.05-0.1wt%, 0.5-2wt%, 0.1-0.5wt% and 2-4wt%. A reagent in the detection kit isa liquid and is stable and inter-bottle difference of the reagent as a result of lyophilization and re-dissolving in preparation and using processes is avoided, so that a relatively great difference among determining results is avoided. The reagent does not need to be lyophilized to ensure the stability of the reagent, the optimum effectiveness of the opened reagent persists for at least one week,the relatively great difference among experimental results is avoided, and meanwhile, the dosage of the reagent is saved. The reagent can be used if being opened, and the kit is more convenient and simple to operate.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a kit for measuring the thrombin time (TT) of a sample by a coagulation method. Background technique [0002] There are two substances in the blood in the human body to maintain the health of the human body, one is the coagulation substance and the other is the anticoagulant substance. The dynamic balance between the two is the key to the normal body to maintain the blood flow in the body and prevent blood loss. Coagulation is the process by which blood changes from a sol state to a gel state. The lack of anticoagulant substances in the body or abnormal fibrinolysis will lead to the formation of thrombus. With the acceleration of the aging process of the population in today's society, various thrombotic diseases have increasingly become factors that cause disease, death and deteriorate the quality of human life, especially Is heart, cerebrovascular thrombosis disease. Thrombotic ...

Claims

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Application Information

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IPC IPC(8): G01N33/86
Inventor 谭柏清王醒赵志敏
Owner 山东艾科达生物科技有限公司
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