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Method for extracting polysaccharide in sunflower seeds

A technology of sunflower and polysaccharide, applied in the field of biological extraction, achieves the effects of high extraction rate, simple method and time saving

Inactive Publication Date: 2018-01-12
广西钟山县富强雅瑶华香农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the preparation of polysaccharides from sunflower discs and their pharmacodynamic functions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A preparation method of sunflower polysaccharides, comprising the steps of:

[0018] 1) Break the sunflower disk, extract with 95v% ethanol under reflux for 3 times, reflux for 4 hours each time, filter, and take the filter residue for later use;

[0019] 2) The filter residue was extracted twice with water under reflux, each time for 4 hours, filtered, and the filtrate was collected for subsequent use;

[0020] 3) Concentrate the filtrate to an extract with a relative density of 1.2 to 1.3 at 60°C, add ethanol for precipitation, and centrifuge to obtain a precipitate;

[0021] 4) Wash the precipitate with absolute ethanol and acetone in turn, dissolve the washed precipitate with distilled water, pass through a hollow fiber membrane with a molecular weight cut-off of 20,000, collect the first concentrate, and then pass the first concentrate For a hollow fiber membrane with a molecular weight of 100,000, collect the second permeate and the second concentrate respectivel...

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PUM

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Abstract

The invention discloses a method for extracting polysaccharide in sunflower seeds, belongs to the technical field of biological extraction, and aims to provide a method for extracting sunflower seed polysaccharide, of which the extraction rate is 3% higher than that of a conventional method. The method comprises the following steps: crushing sunflower discs, performing backflow extraction 3 timeswith 59v% ethanol for 4 hours each time, and filtering to obtain filter residue; performing backflow extraction on the filter residue twice with water for 4 hours each time, filtering, and collectingfiltrate; concentrating the filtrate to obtain an extract with the relative density being 1.2-1.3 at 60 DEG C, performing precipitation with ethanol, and centrifuging to obtain a precipitate; washingthe precipitate with absolute ethyl alcohol and acetone in sequence respectively, concentrating the washed precipitate, centrifuging, and filtering to obtain filtrate; adding activated carbon for decoloring the filtrate, filtering, and performing spray drying on the filtrate, thereby obtaining sunflower polysaccharide. The sunflower polysaccharide prepared with the method can be used for preparinghealthcare foods and medicines, and is particularly applied to preparations for immune adjustment.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, in particular to a method for extracting polysaccharides in sunflower. Background technique [0002] Sunflower (Helianthus annuus L.) is a plant of the genus Helianthus in the family of Asteraceae. As an important oil plant, sunflower is widely planted all over the world. Sunflower is sweet in taste, warm in nature, and non-toxic. In addition to being a source of oil, it also has good pharmacodynamic functions such as calming the liver and expelling wind, clearing dampness and heat, and eliminating stagnant qi. Its seeds, flower discs, stems and leaves, stem marrow, Roots, flowers, etc. can be used as medicine. Seed oil can be used as the basic medicine of ointment. The leaves and petals can be used as a bitter stomachic agent. The flower disc is used to treat gastric cancer, esophageal cancer, malignant hydatidiform mole, headache, dizziness, toothache, stomach pain, menstrual p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 胡锡强
Owner 广西钟山县富强雅瑶华香农业有限公司
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