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Application of indacaterol in treatment of colorectal cancer

A technology for colorectal tumors and tumors, applied in the field of tumor treatment and molecular biology, can solve the problems of reducing cancer lesions, proving the therapeutic effect, and no animal experiments

Active Publication Date: 2018-01-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether indacaterol can treat cancer by regulating alternative splicing to achieve the effect of reducing cancer lesions is not mentioned in the prior art, and there is no animal experiment to prove its therapeutic effect

Method used

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  • Application of indacaterol in treatment of colorectal cancer
  • Application of indacaterol in treatment of colorectal cancer
  • Application of indacaterol in treatment of colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 drug screening:

[0050] The marketed drugs were screened for colorectal cancer tumor-associated alternative splicing events, and it was found that indacaterol (Indacaterol) can regulate colorectal cancer-associated alternative splicing events, thereby inhibiting its role in colorectal cancer.

Embodiment 2

[0051] The cytology experiment of embodiment 2 indacaterol:

[0052] The well-cultured SW620 cell line was taken, and different concentrations of indacaterol were administered to it, and the toxicity of indacaterol to the cell line, as well as the proliferation, invasion, and migration of the cells were observed. First, we found that indacaterol is cytotoxic, and its IC50 on SW620 cells was 19.01 μM (see figure 1 a); and it has a concentration-dependent inhibitory effect on the proliferation of SW620 cells (see figure 1 b); at the same time, it has a significant inhibitory effect on the migration and invasion ability of SW620 (see figure 1 c).

Embodiment 3

[0053] Embodiment 3 The relationship between indacaterol and alternative splicing:

[0054] Based on the results of homology modeling in Example 1 and the relationship between alternative splicing and tumors known to those skilled in the art, we predict that indacaterol is related to the alternative splicing of tumors, so we speculate that when indacaterol is administered to cells Indacaterol can change the alternative splicing of cells. Therefore, we used PCR to test the changes of 6 alternative splicing in SW620 cells administered with indacaterol. The results are as follows: figure 2 shown. That is, the detection of common gene alternative splicing and splicing events in cells, in which DMSO was used as a negative control, the results showed that the abnormal alternative splicing of extracellular matrix protein 1 (Extracellular Matrix Protein1, Ecm1) was corrected after drug treatment; The same effect also includes: skeletal muscle growth homologue 5 (USMG5); peptidylprol...

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Abstract

The invention discloses an application of indacaterol in the treatment of colorectal cancer, and belongs to the field of molecular biology, in particular, tumor treatment. Specifically, indacaterol selected from disclosed drugs can treat colorectal cancer. The results of cell experiments and animal experiments show that indacaterol can inhibit the proliferation, invasion and migration of colorectal tumor cell lines, and can obviously reduce the size of colorectal tumors of a mouse model. At the same time, for the first time, people find that indacaterol corrects the alternative splicing of tumor cells to achieve the effects mentioned above.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to the field of tumor treatment, and relates to a series of therapeutic drugs screened according to markers specifically expressed in colorectal cancer. Specifically, the present invention utilizes gene alternative splicing for the first time to screen a drug for treating colorectal cancer, specifically, the drug is Indacaterol. Background technique [0002] Colorectal cancer is one of the malignant tumors that poses a great threat to human survival and health in the world today, ranking third in the cancer death spectrum in western developed countries. In my country, with the change of residents' lifestyle and dietary structure, the incidence of colorectal cancer is increasing at an annual rate of 4%, and the average annual growth rate is 2 percentage points higher than that of the world. [0003] The Human Genome Project found that the human body has about 23,000 genes, far fewer...

Claims

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Application Information

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IPC IPC(8): A61K31/4704A61P35/00
Inventor 张红河万乐栋来茂德
Owner ZHEJIANG UNIV
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