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Method for generating seedlings directly from Idesia polycarpa Maxim seed embryos

A technology of seeds and hairy leaves, which is applied in the field of Jatropha jatropha planting, can solve the problem of lack of Jatropha jatropha seedlings, shorten the seedling cultivation time and cycle, not easily degenerate, and have good consistency and uniformity of seedlings Effect

Active Publication Date: 2018-01-19
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a method for direct seedling growth of Jatropha jatropha seed embryos, which can quickly produce high-quality seedlings of Jatropha jatropha, improve the propagation speed and seedling efficiency of the seedlings, and realize the seedling factory It can guarantee the quality and uniformity of seedlings, and solve the problem of lack of Jatropha seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Explant treatment and disinfection

[0024] Choose healthy, plump, non-damaged Jatropha seeds as explants, wrap the seeds with clean gauze, put them into 1% (w / w) washing powder solution, soak and gently scrub the seeds for 2-3 minutes to remove For attachments such as dust and wax on the surface, rinse with tap water for 3-4 times, then rinse with running water for 20-25 minutes, transfer to sterile filter paper to absorb surface moisture, and then place in 70% (v / v) ethanol solution for treatment 25-30 seconds, then soak in 0.1% (w / w) mercuric chloride solution for 10-15 minutes, and shake once every 2-3 minutes during this period. After the above 0.1% (w / w) mercuric chloride solution is processed, it is taken out after rinsing with sterile water for 1-2 times, and the surface moisture is slightly blotted dry with sterile filter paper.

[0025] (2)Seed secondary softening

[0026] Step (1) Treat the seeds and soak them in 20% (v / v) hydrogen peroxide solution for...

Embodiment 2

[0036] (1) Explant treatment and disinfection

[0037] Choose healthy, plump, non-damaged Jatropha seeds as explants, wrap the seeds with clean gauze, put them into 1% (w / w) washing powder solution, soak and gently scrub the seeds for 2-3 minutes to remove For attachments such as dust and wax on the surface, rinse with tap water for 3-4 times, then rinse with running water for 20-25 minutes, transfer to sterile filter paper to absorb surface moisture, and then place in 70% (v / v) ethanol solution for treatment 25-30 seconds, then soak in 0.1% (w / w) mercuric chloride solution for 10-15 minutes, and shake once every 2-3 minutes during this period. After the above 0.1% (w / w) mercuric chloride solution is processed, it is taken out after rinsing with sterile water for 1-2 times, and the surface moisture is slightly blotted dry with sterile filter paper.

[0038] (2)Seed secondary softening

[0039] Step (1) Treat the seeds and soak them in 20% (v / v) hydrogen peroxide solution for...

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PUM

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Abstract

The invention discloses a method for generating seedlings directly from Idesia polycarpa Maxim seed embryos. The method comprises the following steps: pre-treating seeds, dissecting the seeds, removing seed coat, and obtaining complete and full seed embryos; inducing the obtained seed embryos into aseptic seedlings, then performing the subculture multiplication induction to obtain clustering seedlings, slitting the clustering seedlings by using an aseptic knife to obtain a single-bud seedling, and inoculating the single-bud seedling in a rooting culture medium to obtain the rooting seedlings.The obtained Idesia polycarpa seed embryos are vigorous in growth, high in seedling rate, high in quality, difficult in degeneration, short in cultivation time and cyclic period which is only 57 to 73days from the planting of the seed embryos to the rooting seedlings, thereby greatly saving the cost; and in addition, the method provided by the invention is simple and easy, the formula of variousinduction culture media, multiplication culture media and rooting culture media is simple and high in operability and repetition. By adopting the method, the difficulty of the low breeding efficiencyof the Idesia polycarpa Maxim is successfully solved, the industrialized production of the seedling is realized, and the market application prospect is good.

Description

technical field [0001] The invention belongs to the technical field of Jatropha jatropha planting, and in particular relates to a method for directly growing seedlings of Jatropha jatropha seed embryos. Background technique [0002] Idesia polycarpa Maxim.var.vestitaDiels (scientific name: Idesia polycarpaMaxim.var.vestitaDiels) is a deciduous tree belonging to the genus Idesia polycarpaMaxim. It is one of the varieties of Idesia polycarpa Maxim. Compared with jatropha, the fruit of jatropha has a large yield, high oil content, good oil quality, and is edible. The fruit production period lasts for more than 50 years, and the dry fruit output per plant reaches more than 75Kg during the rich production period. The oil content of the dried fruit is more than 38%. It can not only produce high-quality edible oil, but also an excellent raw material for extracting linoleic acid, linolenic acid, and vitamins to produce biodiesel, high-grade lubricating oil, skin care soap, and healt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王丽花苏艳瞿素萍张艺萍王继华唐艺容杨秀梅张丽芳许凤吴旻
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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