Donkey-hide gelatin active peptide, composition and preparation method

A technology for active peptides and compositions, applied in the biological field, can solve the problems that the rotational speed of an industrial centrifuge cannot be reached, is difficult to conceal, and is not suitable for industrial production, etc., and achieves the effects of better overall taste, high extraction rate, and improved overall taste.

Inactive Publication Date: 2018-02-09
林峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The donkey-hide gelatin active peptide obtained by this method has obvious bitterness and peculiar taste, even if adding flavoring agent, it is difficult to cover up
And this method is not suitable for industrial production, because the speed of industrial centrifuges cannot meet the requirements of this patent

Method used

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  • Donkey-hide gelatin active peptide, composition and preparation method

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preparation example Construction

[0031]

[0032] In order to eliminate the bitterness and peculiar smell of donkey-hide gelatin active peptide, the preparation method of donkey-hide gelatin active peptide of the present invention comprises the steps:

[0033](1) carrying out enzymolysis treatment to donkey-hide gelatin powder to obtain an enzymolysis solution, and then performing an enzyme-inactivating treatment to obtain an enzymolysis solution after inactivating the enzyme;

[0034] (2) Filter the enzymolysis solution after the deenzyme is eliminated through a plate and frame filter press, and then filter through a ceramic membrane to obtain the active peptide permeate; the plate and frame filter press contains a combined filter aid, and the The combined filter aid is composed of activated carbon, perlite and diatomite in a weight ratio of 6-7:1-2:2-3;

[0035] (3) The active peptide filtrate is concentrated and spray-dried to obtain donkey-hide gelatin active peptide powder; wherein, the spray-drying is ...

Embodiment 1

[0058] The preparation method of donkey-hide gelatin active peptide, comprises the following steps:

[0059] (1) Carrying out enzymolysis treatment of donkey-hide gelatin to obtain an enzymolysis solution, the enzymolysis treatment includes:

[0060] A, dry donkey-hide gelatin is carried out ultrafine pulverization, cross 400 mesh sieves, obtain donkey-hide gelatin powder; Add donkey-hide gelatin powder to the water of 4 times of weight of donkey-hide gelatin powder, mix, adjust to pH8 with sodium hydroxide solution, soak 20h; Stir, centrifuge, and take the supernatant;

[0061] B. The supernatant is adjusted to a pH value of 5 with acetic acid solution, the protein is precipitated, mechanically stirred, centrifuged, and the solid part is taken;

[0062] C. Add water to the solid part, heat at 90°C for 20 minutes, and then homogenize to obtain a suspension;

[0063] D. Adjust the pH value of the suspension to 8, add an enzyme preparation for enzymolysis to obtain an enzymoly...

Embodiment 2

[0068] The preparation method of donkey-hide gelatin active peptide, comprises the following steps:

[0069] (1) Carrying out enzymolysis treatment of donkey-hide gelatin to obtain an enzymolysis solution, the enzymolysis treatment includes:

[0070] A, dry donkey-hide gelatin is carried out ultrafine pulverization, cross 400 mesh sieves, obtain donkey-hide gelatin powder; Add donkey-hide gelatin powder to the water of 4 times of weight of donkey-hide gelatin powder, mix, adjust to pH9 with sodium hydroxide solution, soak 24h; Stir, centrifuge, and take the supernatant;

[0071] B. The supernatant is adjusted to a pH value of 4 with a citric acid solution, the protein is precipitated, mechanically stirred, centrifuged, and the solid part is taken;

[0072] C. Add water to the solid part, heat at 80°C for 30min, and then homogenize to obtain a suspension;

[0073] D. Adjust the pH value of the suspension to 6, add an enzyme preparation for enzymolysis to obtain an enzymolysis s...

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Abstract

The invention provides donkey-hide gelatin active peptide, a composition and a preparation method. The preparation method of the donkey-hide gelatin active peptide comprises the following steps: (1) performing enzymolysis treatment on donkey-hide gelatin powder to obtain an enzymolysis solution, and deactivating enzymes to obtain an enzymolysis solution with the enzymes deactivated; (2) filteringthe enzymolysis solution with the enzymes deactivated through a frame filter press, and filtering through a ceramic membrane to obtain active peptide filtrate; wherein the frame filter press comprisesa combined filter aid, the combined filter aid comprises activated carbon, perlite and diatomaceous earth in a weight ratio of 6-7: 1-2: 2-3; (3) concentrating the active peptide filtrate and spray-drying at 80-105 DEG C to obtain donkey-hide gelatin active peptide powder. The donkey-hide gelatin active peptide has high activity and no bitter taste.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an active peptide of donkey-hide gelatin, a composition and a preparation method. Background technique [0002] Donkey-hide gelatin began in the Qin and Han Dynasties, and is a traditional nourishing and blood-enriching top grade. It is made from donkey skin as the main raw material and water from the well. Donkey-hide gelatin has multiple functions, nourishing blood and nourishing yin, moistening dryness, and stopping bleeding. For blood deficiency and chlorosis, dizziness and palpitations, vexation and insomnia, dryness of lung and cough. The efficacy and role of donkey-hide gelatin in blood and treatment of blood diseases: patients with anemia caused by iron deficiency, blood loss, nutrition, aplastic anemia and other reasons. Applicable to blood deficiency dizziness, heart palpitations, etc. According to clinical observations, donkey-hide gelatin has a better effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A23L33/18A23L33/105
CPCA23L33/105A23L33/18A23V2002/00C12P21/06A23V2200/30A23V2250/55A23V2250/548
Inventor 林峰
Owner 林峰
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