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Peony underground bud bare-handed slicing method and application thereof

A free-hand slicing and peony technology, which is applied in the preparation of test samples, material analysis and sampling by optical means, etc., to achieve the effects of saving scientific research funds, high cutting efficiency, and simple tools

Inactive Publication Date: 2018-02-13
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The previous method of slicing peony flower buds could not fully meet the above conditions, so it showed certain deficiencies.

Method used

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  • Peony underground bud bare-handed slicing method and application thereof
  • Peony underground bud bare-handed slicing method and application thereof
  • Peony underground bud bare-handed slicing method and application thereof

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Embodiment Construction

[0020] Embodiments of the present invention will be further described in detail below in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but should not be used to limit the scope of the present invention.

[0021] From the beginning of July to the end of March of the following year, we observed and sampled the peony buds planted in the experimental nursery of the National Flower Engineering Center (Xiaotangshan Town, Changping District, Beijing). At this time, the peony was in the stage of underground bud development. The sampled varieties are "Dafugui", "Fenyunu", "Zongshengfen" and "Zinnabar". Use a small shovel to shovel off the soil covering the base of the peony stem until the buds that grow at the base are exposed, remove the soil and debris around the buds, and then use a scalpel to completely cut off the buds from the base and place them in a water-soaked container. Absorbent paper (the wate...

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Abstract

The invention relates to the field of biological slicing, in particular to a peony underground bud bare-handed slicing method and application thereof. The bare-handed slicing method comprises the following steps of (1) flatly cutting the bud base part of the peony underground bud in a direction vertical to the growth direction; cutting the bud base part into a cuboid shape; (2) transversely putting the underground bud treated by the step (1) onto a tabletop; vertically cutting the middle of the bud base part in the growth direction by a cutter blade, and performing advancing towards the growthpoint of the underground bud until the underground bud is completely cut into two halves. According to the application of the bare-handed slicing method, the cutting surfaces of the two halves of budslices face the upward direction and are dried for 4 to 6 min at the room temperature; then, the bud slices are put under an anatomical lens to be observed. The slicing method has the advantages thatthe operation is simple; the research funding is reduced; in addition, the use of chemical reagents is not needed; green and environment-friendly effects are achieved; convenience is provided for thepeony blossom bud differentiation study; the reference basis is also provided for the flower bud differentiation study of other plants with the similar flower buds.

Description

technical field [0001] The invention relates to the field of biological slicing, in particular to a free-hand slicing method and application of underground buds of peony. Background technique [0002] Peony belongs to the Paeoniaceae Paeoniaceae plant. Its flowers are large, colorful and rich in flower patterns. It has been loved by the people of our country since ancient times. It is a traditional famous flower in our country. As an ornamental flower, the flower is the most important ornamental part of the peony, and it is also an important carrier for the continuous cultural inheritance of the peony for thousands of years. Flower bud differentiation is a very important stage in the growth and development of flowering plants. Flower bud differentiation has a direct impact on the quantity, quality and yield of flowering of ornamental plants. For peonies, flower bud differentiation is an important process to complete the life cycle and reproduce the population. The differenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N1/28
CPCG01N1/286G01N21/84G01N2001/2873G01N2021/8466
Inventor 于晓南张建军张启翔
Owner BEIJING FORESTRY UNIVERSITY
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