Method for establishing novel mouse coronarospasm model

A coronary artery spasm and mouse model technology, applied in the field of experimental animals, can solve the problems of difficult and complicated operation of vascular endothelial injury, difficulty in capturing the moment of spasm, long time-consuming coronary artery spasm, etc., and achieve low cost of feeding and easy feeding , to avoid the effect of difficult node selection

Inactive Publication Date: 2018-03-16
FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many similarities between this model and clinical cases of coronary artery spasm, so it is widely used in experimental research on the mechanism of coronary artery spasm, but the previous model is difficult to operate and requires high experimental conditions. It takes a long time to induce coronary artery spasm by high-cholesterol diet and other methods in the later stage, and it is not easy to capture the moment of spasm, which has certain limitations on experimental research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for establishing novel mouse coronarospasm model
  • Method for establishing novel mouse coronarospasm model
  • Method for establishing novel mouse coronarospasm model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) 10-week-old C57BL / 6J mice were selected, and lipopolysaccharide was injected intraperitoneally at a weight of 3 mg / kg.

[0030] (2) Ordinary diet, free drinking water, continue to raise in SPF animal room for 6 days;

[0031] (3) Turn on the power of the ECG machine, anesthetize the mouse with pentobarbital sodium at 50 mg / kg body weight, intraperitoneally inject anesthesia, and after about 5 minutes after the anesthesia takes effect, take the mouse supine, and insert the needle electrodes into the subcutaneous of its limbs respectively, The second lead electrocardiogram was recorded by the biological function experiment system;

[0032] (4) Use a 1ml insulin syringe parallel to the tail vein to inject acetylcholine at a rate of 1.5 mg / kg body weight, and observe ST segment elevation in lead II electrocardiogram 5 minutes later.

Embodiment 2

[0034] (1) 10-week-old C57BL / 6J mice were selected, and lipopolysaccharide was injected intraperitoneally at 4 mg / kg body weight.

[0035] (2) Ordinary diet, free drinking water, continue to raise in SPF animal room for 6 days;

[0036](3) Turn on the power of the electrocardiogram machine, anesthetize the mouse with pentobarbital sodium at 45 mg / kg body weight, intraperitoneally inject anesthesia, after about 5 minutes after the anesthesia takes effect, take the mouse supine, and insert the needle electrodes into the subcutaneous of its limbs respectively, The second lead electrocardiogram was recorded by the biological function experiment system;

[0037] (4) Use a 1ml insulin syringe parallel to the tail vein to inject acetylcholine at a rate of 1.5 mg / kg body weight, and observe ST segment elevation in lead II electrocardiogram 3 minutes later.

Embodiment 3

[0039] (1) 10-week-old C57BL / 6J mice were selected, and lipopolysaccharide was injected intraperitoneally at 5 mg / kg body weight.

[0040] (2) Ordinary diet, free drinking water, continue to raise in SPF animal room for 6 days;

[0041] (3) Turn on the power of the electrocardiogram machine, anesthetize the mouse with pentobarbital sodium at 45 mg / kg body weight, intraperitoneally inject anesthesia, after about 5 minutes after the anesthesia takes effect, take the mouse supine, and insert the needle electrodes into the subcutaneous of its limbs respectively, The second lead electrocardiogram was recorded by the biological function experiment system;

[0042] (4) Use a 1ml insulin syringe parallel to the tail vein to inject acetylcholine at a rate of 1mg / kg body weight, and observe ST-segment elevation in lead II electrocardiogram 5 minutes later.

[0043] Negative example:

[0044] (1) Choose 10-week-old C57BL / 6J mice, and inject normal saline intraperitoneally at 3 mg / kg bo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of experiment animals and discloses a method for establishing a novel mouse coronarospasm model. The method is applied to model establishment after in-vivocutaneous damage according to a medicine stimulation method principle. The method is characterized in that intraperitoneal injection of lipopolysaccharide into mice is carried out to result blood vessel cutaneous damage, general diet is supplied, water is taken freely, the mice are continuously cultured for 3-6 days, intraperitoneal injection of pentobarbital sodium for anesthesia is carried out,electrocardiogram is implemented on the mice, intravenous injection of acetyl choline is carried out to induce coronarospasm, whether a II lead electrocardiograph has ST section variation is observed3-5 minutes later, and if ST section rising is resulted, the model is successfully established. Multiple aspects such as testing difficulties, testing conditions, testing periods and economy are greatly improved when being compared with those of conventional models, stable animal models with good repeatability and mechanisms relatively close to that of clinical cases can be provided, and very good animal model samples are provided for study on molecule mechanisms, pathological physiology characteristics and medicine research and development of coronarospasm.

Description

technical field [0001] The invention belongs to the technical field of experimental animals and relates to a method for establishing a novel mouse model of coronary artery spasm. Background technique [0002] Coronary artery spasm (coronary artery spasm, CAS) refers to the transient contraction of the subepicardial artery, causing partial or complete blockage of the blood vessel, which can cause variant angina, unstable angina, acute myocardial infarction, sudden death, etc. Consume medical and social resources. As early as 1845, Latham proposed that coronary artery spasm can lead to angina pectoris. In 1959, Prinzmetal et al. first observed a group of patients with angina pectoris different from exertional angina pectoris, which often occurred in a resting state, accompanied by ST-segment elevation in the electrocardiogram, and named it variant angina. Such patients are not accompanied by increased myocardial oxygen consumption, which is due to insufficient myocardial blo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/739A61K31/221
CPCA61K9/0019A61K31/221A61K31/739
Inventor黄榕翀龚丽莹宋现涛
OwnerFIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV