A method for establishing a mouse model of coronary artery spasm

A technology of coronary artery spasm and mouse model, applied in the field of experimental animals, can solve the problems of difficult and complicated operation of vascular endothelial injury, difficult to capture the time of spasm occurrence, and long time for coronary artery spasm. Low conditions and equipment requirements, highly reproducible results

Inactive Publication Date: 2020-03-31
FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many similarities between this model and clinical cases of coronary artery spasm, so it is widely used in experimental research on the mechanism of coronary artery spasm, but the previous model is difficult to operate and requires high experimental conditions. It takes a long time to induce coronary artery spasm by high-cholesterol diet and other methods in the later stage, and it is not easy to capture the moment of spasm, which has certain limitations on experimental research

Method used

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  • A method for establishing a mouse model of coronary artery spasm
  • A method for establishing a mouse model of coronary artery spasm
  • A method for establishing a mouse model of coronary artery spasm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Select 10-week-old C57BL / 6J mice, and inject lipopolysaccharide intraperitoneally according to the body weight of 3 mg / kg.

[0030] (2) Ordinary diet, free drinking water, and continue raising in SPF animal room for 6 days;

[0031] (3) Turn on the power of the electrocardiograph, inject the mice with pentobarbital sodium at a body weight of 50 mg / kg, and anesthetize them by intraperitoneal injection. After the anesthesia takes effect for about 5 minutes, take the mice supine, and pierce the needle electrodes into their limbs subcutaneously. Record lead II electrocardiogram through biological function experiment system;

[0032] (4) A 1ml insulin syringe was inserted into the needle in parallel with the tail vein, and acetylcholine was injected at 1.5mg / kg body weight. After 5 minutes, ST-segment elevation in lead II electrocardiogram was observed.

Embodiment 2

[0034] (1) 10-week-old C57BL / 6J mice were selected, and lipopolysaccharide was injected intraperitoneally according to the body weight of 4 mg / kg.

[0035] (2) Ordinary diet, free drinking water, and continue raising in SPF animal room for 6 days;

[0036](3) Turn on the power of the electrocardiograph, inject the mice with pentobarbital sodium at a body weight of 45 mg / kg, and anesthetize them by intraperitoneal injection. After the anesthesia takes effect for about 5 minutes, take the mice in a supine position, and pierce the needle electrodes into their limbs subcutaneously. Record lead II electrocardiogram through biological function experiment system;

[0037] (4) Insert a 1ml insulin syringe parallel to the tail vein, inject acetylcholine at 1.5mg / kg body weight, and observe ST-segment elevation in lead II ECG 3 minutes later.

Embodiment 3

[0039] (1) 10-week-old C57BL / 6J mice were selected, and lipopolysaccharide was injected intraperitoneally according to the body weight of 5 mg / kg.

[0040] (2) Ordinary diet, free drinking water, and continue raising in SPF animal room for 6 days;

[0041] (3) Turn on the power of the electrocardiograph, inject the mice with pentobarbital sodium at a body weight of 45 mg / kg, and anesthetize them by intraperitoneal injection. After the anesthesia takes effect for about 5 minutes, take the mice in a supine position, and pierce the needle electrodes into their limbs subcutaneously. Record lead II electrocardiogram through biological function experiment system;

[0042] (4) A 1ml insulin syringe was inserted into the needle in parallel with the tail vein, and acetylcholine was injected at 1mg / kg body weight. After 5 minutes, ST segment elevation in lead II electrocardiogram was observed.

[0043] Negative example:

[0044] (1) 10-week-old C57BL / 6J mice were selected, and the bod...

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Abstract

The invention belongs to the technical field of experiment animals and discloses a method for establishing a novel mouse coronarospasm model. The method is applied to model establishment after in-vivocutaneous damage according to a medicine stimulation method principle. The method is characterized in that intraperitoneal injection of lipopolysaccharide into mice is carried out to result blood vessel cutaneous damage, general diet is supplied, water is taken freely, the mice are continuously cultured for 3-6 days, intraperitoneal injection of pentobarbital sodium for anesthesia is carried out,electrocardiogram is implemented on the mice, intravenous injection of acetyl choline is carried out to induce coronarospasm, whether a II lead electrocardiograph has ST section variation is observed3-5 minutes later, and if ST section rising is resulted, the model is successfully established. Multiple aspects such as testing difficulties, testing conditions, testing periods and economy are greatly improved when being compared with those of conventional models, stable animal models with good repeatability and mechanisms relatively close to that of clinical cases can be provided, and very good animal model samples are provided for study on molecule mechanisms, pathological physiology characteristics and medicine research and development of coronarospasm.

Description

technical field [0001] The invention belongs to the technical field of experimental animals, and relates to a method for establishing a novel coronary artery spasm mouse model. Background technique [0002] Coronary artery spasm (CAS) refers to the transient contraction of the subepicardial arteries, causing partial or complete occlusion of the blood vessels, which can cause variant angina pectoris, unstable angina pectoris, acute myocardial infarction, sudden death, etc. Consume medical and social resources. As early as 1845, Latham proposed that coronary artery spasm can lead to angina pectoris. In 1959, Prinzmetal et al first observed a group of patients with angina pectoris different from exertional angina pectoris, often attacking in the resting state, accompanied by ST-segment elevation on the electrocardiogram, and named it variant angina pectoris. Such patients are not accompanied by an increase in myocardial oxygen consumption, which is caused by myocardial insuff...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A61K31/739A61K31/221
CPCA61K9/0019A61K31/221A61K31/739
Inventor黄榕翀龚丽莹宋现涛
OwnerFIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV