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Paeonia suffruticosa seed bioactive peptide and preparation method

A technology of peony seeds and active peptides, which is applied in the field of peony seed active peptides and its preparation, can solve the problems that are not conducive to the purification and taste of peony peptides, and achieve the effect of improving health care functions

Inactive Publication Date: 2018-03-27
林峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is not conducive to the purification and taste of peony peptide

Method used

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  • Paeonia suffruticosa seed bioactive peptide and preparation method
  • Paeonia suffruticosa seed bioactive peptide and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation method of peony seed active peptide, comprising the following steps:

[0039] A. The peony seeds are pretreated, ultrafinely pulverized, passed through 400 meshes, mixed with water, adjusted to pH 8-9 for slightly alkaline treatment, and soaked for 20 hours. Mechanical stirring, centrifugation of the material and liquid in the reaction tank, and collecting the supernatant;

[0040] B. Add acetic acid to the supernatant to precipitate protein. After adjusting the pH value of the supernatant to 4-6, mechanically agitate and centrifuge to collect the precipitate, which is the peony seed protein;

[0041] C. Redissolve the precipitate with water, heat to 90 degrees to denature the protein, and then perform high-pressure homogenization;

[0042] D. Adjust the pH of the above solution to 7-8, add special enzyme preparation flavored protease: trypsin: pepsin at a ratio of 1.5: 1.5: 2.5, and perform enzymolysis. The enzymolysis temperature is 50° C., and the enz...

Embodiment 2

[0055] A preparation method of peony seed active peptide, comprising the following steps:

[0056] A. Pretreat the dried peony seeds, ultrafinely pulverize them, pass through 400 meshes, add water to mix, adjust the pH to 8-9 for slightly alkaline treatment, and soak for 20 hours. Mechanical stirring, centrifugation of the material and liquid in the reaction tank, and collecting the supernatant;

[0057] B. Add acetic acid to the supernatant to precipitate protein. After adjusting the pH value of the supernatant to 4-6, mechanically agitate and centrifuge to collect the precipitate, which is the peony seed protein;

[0058] C. Redissolve the precipitate with water, heat to 90 degrees to denature the protein, and then perform high-pressure homogenization;

[0059] D. Adjust the pH of the above solution to 7-8, add special enzyme preparation flavored protease: trypsin: pepsin at a ratio of 1.4: 1.6: 2.2, enzymolysis, the enzymolysis temperature is 50 ° C, and the enzymolysis t...

Embodiment 3

[0072] A preparation method of peony seed active peptide, comprising the following steps:

[0073] A. Pretreat the dried peony seeds, ultrafinely pulverize them, pass through 400 meshes, add water to mix, adjust the pH to 8-9 for slightly alkaline treatment, and soak for 22 hours. Mechanical stirring, centrifugation of the material and liquid in the reaction tank, and collecting the supernatant;

[0074] B. Add acetic acid to the supernatant to precipitate protein. After adjusting the pH value of the supernatant to 4-6, mechanically agitate and centrifuge to collect the precipitate, which is the peony seed protein;

[0075] C. Redissolve the precipitate with water, heat to 95 degrees to denature the protein, and then perform high-pressure homogenization;

[0076] D. Adjust the pH of the above solution to 7-8, add special enzyme preparation flavored protease: trypsin: pepsin at 1.6: 1.6: 2.4, enzymolysis, enzymolysis temperature is 55 ° C, and enzymolysis time is 1.5h;

[00...

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Abstract

The invention provides a preparation method of paeonia suffruticosa seed bioactive peptide. The preparation method comprises the following steps of preparing the refined paeonia suffruticosa seed bioactive peptide and fully nutritive paeonia suffruticosa seed bioactive peptide through paeonia suffruticosa seeds as raw materials. An enzyme preparation is combined with high-efficient enzymolysis, sothat the energy consumption and material consumption are reduced; during leaching and enzyme deactivation, traditional Chinese medicines of mulberry leaves and perilla fruits are creatively added, sothat the nutrient components of the paeonia suffruticosa seed peptide are further enriched; in the separation process, a specific filtration addition agent combination is adopted, so that the separation refining power is greatly strengthened; and finally, a medium and low temperature spray drying tower is adopted, heat-sensitive active substances are protected, and the medium and low temperaturespray drying tower is also firstly applied to a peptide technology. In addition, according to the preparation method, the raw materials are easy to obtain, the production technology is simple, and theproduction cost is greatly reduced. The mass content of the prepared refined paeonia suffruticosa seed bioactive peptide is greater than or equal to 80%, and the mass content of the fully nutritive paeonia suffruticosa seed bioactive peptide is greater than or equal to 30%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a peony seed active peptide and a preparation method. Background technique [0002] Peony is a plant of Ranunculaceae and Paeonia genus, which is a small perennial deciduous shrub. The root of peony is processed into "Paeon Cortex", which is a valuable Chinese herbal medicine. It is mildly cold in nature, pungent in taste, non-toxic, enters the three meridians of heart, liver, and kidney, and has the effects of dispelling blood stasis, clearing blood, harmonizing peony blood, relieving pain, stimulating menstrual flow, lowering blood pressure, antibacterial and anti-inflammatory effects. Long-term use can benefit the body and prolong life, nourish blood and liver, dispel stagnation and stagnation. It is suitable for facial chloasma and skin aging. Regular drinking of qi and blood can activate the lungs, make the face rosy, improve menstrual disorders, dysmenorrhea, and r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00
CPCA23J1/006
Inventor 林峰
Owner 林峰
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