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Tea seed bioactive peptide and preparation method

A technology of active peptides and tea seeds, applied in protein food processing, food science, protein food ingredients, etc., can solve problems such as high cost, unsuitable for industrial production, and unfavorable for full enzymatic hydrolysis, and achieve the effect of improving health care function

Inactive Publication Date: 2018-01-26
林峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the use of freeze-drying is not conducive to large-scale industrial production
Publication No. CN102010460B announced "A Camellia Seed Polypeptide and Its Preparation Method". Using a single enzymatic hydrolysis protein is not conducive to sufficient enzymatic hydrolysis. In addition, H+ type cation exchange resin and OH- type anion exchange resin are used to remove the protein during filtration. Anion, its cost is huge, not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A preparation method of tea seed active peptide, comprising the following steps:

[0037] A. The tea seeds are pretreated, ultrafinely pulverized, passed through 400 meshes, mixed with water, adjusted to pH 8-9 for slightly alkaline treatment, and soaked for 20 hours. Mechanical stirring, centrifugation of the material and liquid in the reaction tank, and collecting the supernatant;

[0038] B. Add acetic acid to the supernatant to precipitate protein. After adjusting the pH value of the supernatant to 4-5, mechanically stir and centrifuge to collect the precipitate, which is the tea seed protein;

[0039] C. Redissolve the precipitate with water, heat to 90 degrees to denature the protein, and then perform high-pressure homogenization;

[0040] D. Adjust the pH of the above solution to 7-8, add a special enzyme preparation (flavor protease: papain: neutral protease is 3.5: 2.5: 1), enzymolysis, enzymolysis temperature is 50 ° C, enzymolysis time is 2h;

[0041] E. A...

Embodiment 2

[0053] A preparation method of tea seed active peptide, comprising the following steps:

[0054] A. Pretreat the dry tea seeds, carry out superfine grinding, pass through 400 meshes, add water to mix, adjust the pH to 8-9 for micro-alkaline treatment, and soak for 20 hours. Mechanical stirring, centrifugation of the material and liquid in the reaction tank, and collecting the supernatant;

[0055] B. Add acetic acid to the supernatant to precipitate protein. After adjusting the pH value of the supernatant to 4-6, mechanically stir and centrifuge to collect the precipitate, which is the tea seed protein;

[0056] C. Redissolve the precipitate with water, heat to 90 degrees to denature the protein, and then perform high-pressure homogenization;

[0057] D. Adjust the pH of the above solution to 7-8, add special enzyme preparation (flavor protease: papain: neutral protease is 3.4: 2.6: 1.2), enzymolysis, enzymolysis temperature is 50 ℃, enzymolysis time is 2h;

[0058] E. Afte...

Embodiment 3

[0070] A preparation method of tea seed active peptide, comprising the following steps:

[0071] A. The dry tea seeds are pretreated, ultrafinely pulverized, passed through 400 meshes, mixed with water, adjusted to pH 8-9 for slightly alkaline treatment, and soaked for 22 hours. Mechanical stirring, centrifugation of the material and liquid in the reaction tank, and collecting the supernatant;

[0072] B. Add acetic acid to the supernatant to precipitate protein. After adjusting the pH value of the supernatant to 4-6, mechanically stir and centrifuge to collect the precipitate, which is the tea seed protein;

[0073] C. Redissolve the precipitate with water, heat to 95 degrees to denature the protein, and then perform high-pressure homogenization;

[0074] D. Adjust the pH of the above solution to 7-8, add special enzyme preparation (flavor protease: papain: neutral protease is 3.6: 2.6: 1.3), enzymolysis, enzymolysis temperature is 55 ℃, enzymolysis time is 1.5h;

[0075] ...

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Abstract

The invention provides a preparation method of tea seed bioactive peptide. According to the preparation method, tea seeds are used as raw materials, and refined tea seed bioactive peptide and whole-nutrition tea seed bioactive peptide are prepared. An enzyme preparation is combined with high-efficient enzymolysis, so that energy consumption and material consumption are reduced; in addition, duringleaching enzyme deactivation, traditional Chinese medicines of grand torreya seeds and Chinese prickly ash are creatively added, so that nutrient components of tea seed peptide are further enriched.In the separation process, a specific filtration addition agent combination is used, so that the refining and separating capacity is greatly strengthened; and finally, a medium and low temperature spray drying tower is used, so that heat-sensitive active substances are protected, and the medium and low temperature spray drying tower is also applied to a peptide technology for the first time. In addition, according to the preparation method disclosed by the invention, the raw materials are easy to obtain, the production technology is simple, and the production cost is greatly reduced. The masscontent of the prepared refined tea seed bioactive peptide is greater than or equal to 80%, and the mass content of the whole-nutrition tea seed bioactive peptide is greater than or equal to 30%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a tea seed active peptide and a preparation method. Background technique [0002] Tea seeds, the seeds of the camellia oleifera tree, can be squeezed for oil. Tea seeds have antioxidant capacity, can lower blood pressure, lower blood lipids and inhibit arteriosclerosis. The nutritional value of tea seeds is very high. Tea seeds contain a large amount of unsaturated fatty acids, which are stable in nature, and their physical and chemical properties change little after one year of sealed storage. Tea seed oil is easier to store than other vegetable oils. Tea seed oil can be used for stir-frying and cold dishes like other edible vegetable oils, and has a unique tea aroma. Tea seed oil is rich in unsaturated and fatty acids. Long-term consumption has obvious curative effects on cardiovascular diseases, lowering serum cholesterol, high blood pressure, cardiovascular and ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/34A23L33/10A23L33/18
Inventor 林峰
Owner 林峰
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