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Leech active peptide, composition and preparation method

The technology of active peptide and composition is applied in the field of preparation of leech active peptide, which can solve the problems of low efficiency of single-enzyme enzymolysis, unfavorable cost and industrialization, etc., and achieve the effect of improving health care function.

Inactive Publication Date: 2018-03-06
林峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Patent CN201710412958.5 discloses "a leech protein polypeptide powder and its application". This invention is prepared through the following steps: pretreatment: take natural leeches, soak in sodium chloride solution; refining: homogenate, filter out impurities, and obtain slurry ;Modification: Treat the slurry with microwaves to obtain a modified solution; Hydrolysis: Add papain to the modified solution to adjust the pH to 4.5-6, combine microwaves to hydrolyze the modified solution, and then cool down to 4-10 ℃, placed at a constant temperature; combined with microwave treatment, stop the reaction, and obtain a hydrolysis solution; inactivation: heat the hydrolysis solution to inactivate; filter: filter the filtrate; dry: dry the filtrate to obtain leech protein polypeptide powder; single enzyme enzymatic hydrolysis efficiency Low, and the use of microwave treatment is boring, which is far higher than heat treatment in terms of energy consumption and equipment requirements, which is not conducive to cost and industrialization

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preparation example Construction

[0023]

[0024] The preparation method of leech active peptide of the present invention comprises the following steps:

[0025] A. Add the leech powder to water, adjust the pH to 8-9, soak for 18-22 hours, separate the solid and liquid, and take the supernatant;

[0026] B. Adjust the pH of the supernatant to 4-6 for precipitation, separate the solid from the liquid, and take the solid part;

[0027] C. Add water to the solid part, heat at 80-100°C for 20-40 minutes, and then homogenize to obtain a suspension;

[0028] D. Adjust the pH of the suspension to 5-9, add an enzyme preparation for enzymolysis; the enzyme preparation is composed of pepsin and papain, and the weight ratio of the pepsin and papain is 3-5: 1~2, the enzymolysis temperature is 40~60℃, the enzymolysis time is 2~7h, and the enzymolysis solution is obtained;

[0029] E. The enzymatic hydrolyzate is deenzyme-treated, and then made into leech active peptide powder.

[0030] According to the preparation met...

Embodiment 1

[0048] The preparation method of leech active peptide comprises the following steps:

[0049] A. The leeches are ultrafinely pulverized and passed through a 400-mesh sieve to obtain leech powder; the leech powder is added to water with 4 times the weight of the leech powder, mixed, adjusted to pH 8 with sodium hydroxide solution, soaked for 20 hours; mechanically stirred, centrifuged , take the supernatant;

[0050] B, the supernatant is adjusted to pH5 with acetic acid solution, precipitated protein, mechanically stirred, centrifuged, and the solid part is taken;

[0051] C. Add water to the solid part, heat at 95°C for 20min, and then homogenize to obtain a suspension;

[0052] D. Adjust the pH of the suspension to 8, add an enzyme preparation for enzymolysis to obtain an enzymolysis solution; the enzyme preparation is composed of pepsin and papain in a weight ratio of 3.5:1.5; the amount of the enzyme preparation is the suspension 1wt% of the weight of solids in the mediu...

Embodiment 2

[0055] The preparation method of leech active peptide comprises the following steps:

[0056] A. The leeches are ultrafinely pulverized and passed through a 350-mesh sieve to obtain leech powder; the leech powder is added to water 3.5 times the weight of the leech powder, mixed, adjusted to pH 9 with sodium hydroxide solution, soaked for 22 hours; mechanically stirred, centrifuged , take the supernatant;

[0057] B. Adjust the supernatant to pH6 with acetic acid solution, precipitate protein, mechanically stir, centrifuge, and get the solid part;

[0058] C. Add water to the solid part, heat at 90°C for 25min, and then homogenize to obtain a suspension;

[0059] D. Adjust the pH of the suspension to 7.5, add an enzyme preparation for enzymolysis to obtain an enzymolysis solution; the enzyme preparation is composed of pepsin and papain in a weight ratio of 3.6:1.7; the amount of the enzyme preparation is the suspension 1.2wt% of the weight of solids in the medium; the enzymol...

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Abstract

The invention provides leech active peptide, a composition and a preparation method. The preparation method comprises the following steps: (A) adding leech powder into water, adjusting the pH value to8-9, soaking for 18-22 hours, and carrying out solid-liquid separation; adjusting the pH value of supernate to 4-6, and carrying out precipitation and solid-liquid separation; adding solids into water, heating at 80-100 DEG C for 20-40 minutes, and carrying out homogenization; adjusting the pH value of obtained suspension to 5-9, and adding an enzyme preparation for enzymolysis, so as to obtain enzymatic hydrolysate, wherein the enzyme preparation contains pepsase and papain in a weight ratio of (3-5) to (1-2), the enzymolysis temperature is 40-60 DEG C, and the enzymolysis time is 2-7 hours;and carrying out enzyme deactivation on enzymatic hydrolysate, so as to obtain the leech active peptide. The active peptide and the composition are capable of enhancing immunity.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of leech active peptide. Background technique [0002] As a traditional Chinese medicine, leeches have a long history. Chinese medicine believes that leeches have the functions of breaking blood, removing stasis, and stimulating menstruation. Studies have shown that leech, a traditional Chinese medicine, has a significant effect of lowering blood lipids, and significantly reduces aortic atherosclerotic plaques. Collagen fibers can be seen in the plaques, and cholesterol crystals are reduced. It provides a certain theoretical basis for the traditional Chinese medicine leech to prevent and treat atherosclerosis and various cardiovascular and cerebrovascular diseases. At the same time, it was also found that treating thrombophlebitis, liver cirrhosis, and respiratory system diseases with Chinese medicine leech has achieved satisfactory results. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A23L33/18A23L33/105
CPCA23L33/105A23L33/18A23V2002/00C12P21/06A23V2200/3262A23V2250/55A23V2250/21A23V2200/324
Inventor 林峰
Owner 林峰
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