Biomimetic membrane for removing aflatoxins, and preparation method and treatment method of biomimetic membrane

A kind of technology of aflatoxin and biomimetic film, which is applied to the device of coating liquid on the surface, fixed on/in the organic carrier, food science, etc. It can solve the problem that bacteria or enzyme reagents cannot be reused and are difficult to disperse uniformly Dosage, destruction of nutrients and other issues, to increase stability and repeated use time, reduce dosage, improve the effect of removal

Active Publication Date: 2018-04-20
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the existing methods of removing aflatoxins, which have high energy consumption, destruction of nutrients, and the inability to re-use the bacterial agent or enzyme reagent of the biological method, and the difficulty of uniform dispersion resulting in large dosage and high cost, the present invention provides a A kind of biomimetic film for removing aflatoxin and its preparation method and treatment method

Method used

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  • Biomimetic membrane for removing aflatoxins, and preparation method and treatment method of biomimetic membrane

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preparation example Construction

[0045] Its preparation method is as figure 1 As shown in steps 1-4, the following steps are included:

[0046] (1) Soak the microporous membrane 1 in a dopamine solution, form a dopamine coating on the surface of the microporous membrane and in the pores, and obtain a microporous membrane 2 coated with a dopamine coating;

[0047] (2) Soak the microporous membrane coated with dopamine coating described in step (1) in polyethyleneimine solution, so that polyethyleneimine and polydopamine in the microporous membrane undergo a covalent bond reaction to obtain an improved Sexual microporous membrane 3;

[0048] (3) Filtrating and / or soaking the enzyme solution in the modified microporous membrane described in step (2), immobilizing the enzyme molecules in the enzyme solution on the modified microporous membrane, and preparing the biomimetic membrane 4 .

[0049] The biomimetic membrane is used to remove the aflatoxin in the solution, and its specific method includes:

[0050] (...

Embodiment 1

[0054] In the present embodiment, the preparation method of the biomimetic membrane that is used for aflatoxin removal is as follows (its preparation process is as follows figure 1 shown), specifically include the following steps:

[0055] (1) Soak the polyvinylidene fluoride microporous membrane 1 with a pore diameter of 1 μm in a dopamine solution (with a concentration of 2 g / L) at pH=8.5 for 2 hours to obtain a dopamine-coated microporous membrane 2;

[0056] (2) Rinse the dopamine-coated microporous membrane 2 with deionized water and soak it in a polyethyleneimine solution (molecular weight 750000Da) at a concentration of 2g / L for 12h at a reaction temperature of 60°C to obtain a modified Microporous membrane 3;

[0057] (3) Filter the aflatoxin oxidase (co-immobilized 0.22 μg / cm2) through the modified microporous membrane 3 2 Enzyme), the biomimetic membrane 4 was prepared.

[0058] The aflatoxin B1 is processed by the biomimetic membrane prepared, and the processing ...

Embodiment 2

[0063] In the present embodiment, the preparation method of the biomimetic membrane that is used for aflatoxin removal is as follows (its preparation process is as follows figure 1 shown), specifically include the following steps:

[0064] (1) Soak the polyamide microporous membrane 1 with a pore size of 0.1 μm in a dopamine solution (concentration of 4 g / L) at pH=9.5 for 1 hour to obtain a microporous membrane 2 coated with a dopamine coating;

[0065] (2) Rinse the dopamine-coated microporous membrane 2 with deionized water, soak it in a polyethyleneimine solution (molecular weight: 600 Da) at a concentration of 4 g / L for 1 hour, and have a reaction temperature of 25° C. to obtain a modified Microporous membrane 3;

[0066] (3) The modified microporous membrane 3 filter laccase (co-immobilized 0.3 μg / cm 2 Enzyme), the biomimetic membrane 4 was prepared.

[0067] The aflatoxin B1 is processed by the biomimetic membrane prepared, and the processing process is as follows: f...

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Abstract

The invention provides a biomimetic membrane for removing aflatoxins, and a preparation method and treatment method of the biomimetic membrane. The biomimetic membrane comprises a microporous membrane, a dopamine coating coats the microporous membrane, polyethyleneimine is grafted to the dopamine coating, and enzyme molecules are adsorbed onto the polyethyleneimine. The method comprises the following steps of firstly coating the microporous membrane with poly-dopamine, then grafting the polyethyleneimine, and finally adsorbing the enzyme molecules through the polyethyleneimine. Enzymes adsorbed onto the biomimetic membrane can be unceasingly desorbed and released into feed liquid in the process of performing catalytic degradation on the aflatoxins, so that the polyethyleneimine is exposedto continue adsorbing the aflatoxins until a saturated state is obtained, and the total aflatoxins removing rate is greater than 80%. The adsorbed aflatoxins are eluted with aqueous alkali, so that regeneration and repeated use of the biomimetic membrane are realized, and 50% or above of the eluted aflatoxins can be degraded in the aqueous alkali. The preparation technology of the biomimetic membrane disclosed by the invention is simple, enzyme catalysis, enzyme slow release, membrane adsorption and alkali treatment are combined, and the removal rate of the aflatoxins can be increased.

Description

technical field [0001] The invention belongs to the field of food detoxification, and relates to a biomimetic film and a preparation method and a treatment method thereof, in particular to a biomimetic film for removing aflatoxin and a preparation method and a treatment method thereof. Background technique [0002] Aflatoxin is mainly a secondary metabolite biosynthesized by complex enzymatic reactions of fungi such as Aspergillus flavus and Aspergillus parasiticus under certain environmental conditions. It is a class of compounds with similar chemical structures, all of which are dihydrofuranocoumarins. There are currently 18 types of derivatives identified, among which aflatoxin B1 is the most toxic substance among aflatoxins. Aflatoxin can contaminate food and feed through many ways, and directly or indirectly enter the human food chain. Aflatoxin mainly acts on the liver and causes liver lesions. Consuming foods containing aflatoxin can cause acute poisoning in humans a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20C12N11/08B05D7/24B05D7/04
CPCA23L5/25A23L5/273B05D7/04B05D7/24C12N11/08
Inventor 罗建泉万印华陈向荣
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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