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A MOF-magnetic graphene hybrid material and its application in chiral resolution and in vitro cytotoxicity

A MOF- and magnetic technology, applied in the intersection of nanomaterials and toxicology, can solve the problems of expensive chiral resolution reagents or chiral catalysts, involving many chemical species, long preparation routes, etc., to achieve good chiral recognition ability, The effect of uniform size and low preparation cost

Inactive Publication Date: 2020-11-06
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation methods of (S)-ibuprofen can be divided into two categories: racemate resolution and asymmetric synthesis, but both have chiral resolution reagents or chiral catalysts that are expensive, long preparation routes, and involve There are many disadvantages such as many chemical species and low ee% value

Method used

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  • A MOF-magnetic graphene hybrid material and its application in chiral resolution and in vitro cytotoxicity
  • A MOF-magnetic graphene hybrid material and its application in chiral resolution and in vitro cytotoxicity
  • A MOF-magnetic graphene hybrid material and its application in chiral resolution and in vitro cytotoxicity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Synthesis of MOF-magnetic graphene hybrid material

[0034] One, the synthetic method of MOF-magnetic graphene hybrid material of the present invention comprises the following steps:

[0035] (1) Preparation of graphene oxide (GO):

[0036] It proceeds in three stages:

[0037] Low-temperature reaction: Weigh 1 g of graphite powder in a 500 mL three-neck flask, add 35 mL of concentrated sulfuric acid in an ice bath, stir for 30 min, cool to below 4°C, and weigh 4 g of KMnO 4 Add slowly, and control the temperature not to exceed 10°C;

[0038] Medium-temperature reaction: move the three-neck flask to a constant temperature oil bath at 35 °C, and stir it magnetically for 12 h;

[0039] High-temperature reaction: slowly add 138 mL of high-purity water to the obtained mixture, keep the temperature of the mixture at 90-95°C for 30 minutes, and keep stirring moderately during the reaction; measure 12.5 mL (30 Vol %) of hydrogen peroxide and slowly add it, and st...

Embodiment 2

[0053] Example 2 Test of Rapid Selective Adsorption of Propranolol Hydrochloride Enantiomers by MOF-Magnetic Graphene Hybrid Material

[0054] One of the advantages of magnetic solid-phase extraction is that it can use an external magnetic field to achieve rapid separation of magnetic materials and solutions.

[0055] The test process done by the present invention is: first, take a certain amount of MOF-magnetic graphene hybrid material synthesized in Example 1 in a centrifuge tube, then add a certain amount of propranolol hydrochloride racemate stock solution to be analyzed and extract solution, ultrasonic vibration extraction for a certain period of time, use the strong magnetic field of the magnet to make the magnetic material gather at the bottom of the centrifuge tube, realize the separation of the material and the solution, discard the supernatant, add a certain amount of eluent to it, and ultrasonically wash once Discard the supernatant. Then add a certain amount of el...

Embodiment 3

[0057] Example 3 Effects of propranolol hydrochloride racemate (Rac-Pro), R-propranolol hydrochloride (R-Pro) and S-propranolol hydrochloride (S-Pro) on the proliferation of A549 cells

[0058] Digest and collect A549 cells in the logarithmic growth phase, adjust the concentration of the cell suspension to about 5×10 4 cells / mL, add 100 μL of cell suspension to each well of a 96-well plate, and place the culture plate at 37°C, 5% CO 2Cultured in the incubator for about 24 h. After the cells adhered to the wall, the culture medium in each well was sucked out, and 100 μL of culture medium containing different concentrations of propranolol hydrochloride, R-propranolol hydrochloride and S-propranolol hydrochloride were added according to the experimental grouping, and the experiment was set as negative at the same time. control group and blank control group. The negative control group was culture medium without drugs, and each group had 6 replicate wells. The culture plate was ...

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Abstract

The invention discloses a synthesis method of an MOF-magnetic graphene hybrid material. According to the method, firstly, graphene oxide and magnetic ferroferric oxide microspheres are prepared; Fe3O4 / polydopamine composite nanometer particles are prepared at room temperature; then, a one-step normal pressure synthesis method is used; the Fe3O4 / polydopamine is dispersed into a graphene oxide solution; copper acetate hydrate, S-malic acid and 4,4'-bipyridine methanol / water solution are gradually added step by step; the MOF-magnetic graphene hybrid material is prepared. The material has the advantages that the dispersivity is good; the structure property is stable; the synthesis method is simple, fast and convenient and the like. Meanwhile, the chiral recognition capability of the material on medicine of propranolole hydrochloride is studied; the repeated use performance of the material is utilized; the R- and S-propranolole hydrochloride enantiomers are respectively prepared; a CCK-8 method is used for performing lung adenocarcinoma cell toxicity detection on the R- and S-propranolole hydrochloride; the action differences of the enantiomers are compared.

Description

technical field [0001] The present invention relates to the interdisciplinary field of nanomaterials and toxicology, in particular to a MOF-magnetic graphene hybrid material, and the present invention also relates to the use of the MOF-magnetic graphene hybrid material in the chiral resolution of propranolol hydrochloride enantiomers. Applications in analysis and in vitro cytotoxicity. Background technique [0002] The separation of chiral compounds, especially chiral drugs, is of great significance in drug research and pharmaceutical industry development. Chiral drugs refer to some pharmacologically active drugs composed of chiral compounds. Due to the different stereo configurations of enantiomers, their interactions are also quite different, which makes the pharmacological activity of chiral drugs exhibit different configurations. , metabolic process and rate, toxicity and other kinetic characteristics are also very different. Taking racemic drugs has great potential da...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/22B01J20/20B01J20/28B01J20/29B01J20/30G01N33/50
CPCB01J20/20B01J20/226B01J20/28009B01J20/29G01N33/5014G01N2800/12
Inventor 于阿娟杜文瑞李艳张书胜赵无垛陈胜
Owner ZHENGZHOU UNIV
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