Ultrasound-assisted simulated digestion method of casein active peptide and its application in health food

Abstract

The invention discloses an ultrasonic sound assistant method for simulated digestion of casein active peptides and health food applications, and belongs to the technical fields of dairy product deep processing and health food preparation. The method comprises the following steps: ultrasonic pretreatment of casein is firstly employed; enzymatic hydrolysis is carried out with protease in order to prepare casein active peptides with ACE inhibitory activity; ACE inhibitory activity of the casein active peptides is traced by means of simulation of gastrointestinal tract digestion, and after Caco-2cells simulate absorption of small intestine epithelial cells, the casein functional polypeptides with high ACE inhibitory activity which are digested by gastrointestinal tracts and absorbed by smallintestine inner walls which are simulated by Caco-2 cells are represented. The simulated digestive system and the small intestine inner wall absorption system simulated by Caco-2 cells are firstly combined in order to trace activity change of casein polypeptides with ACE inhibition; three kinds of casein functional polypeptides with high ACE inhibitory activity which are digested by stomach and intestine and absorbed by small intestine inner walls which are simulated by Caco-2 cells are firstly identified.

Description

technical field The invention provides an ultrasonic-assisted simulated digestion method of casein active peptide and its application in functional food, which belongs to the technical field of deep processing of dairy products and preparation of functional food, and can be used as a functional product for preparing health food or medicine. Background technique Hypertension (hypertension) refers to an increase in systemic arterial blood pressure (systolic and / or diastolic) as the main feature (systolic blood pressure ≥ 140 mm Hg, diastolic blood pressure ≥ 90 mm Hg), which may be accompanied by heart, brain, kidney, etc. Clinical syndrome of functional or organic damage to organs. Hypertension is the most common chronic disease and the main risk factor for cardiovascular and cerebrovascular diseases. At present, there are more than 200 million hypertensive patients in China, so the current situation is very serious. Angiotensin converting enzyme (ACE EC 3.4.15.1) is a Zn-c...

AI Technical Summary

Problems solved by technology

The patent "Anti-inflammatory peptide isolated from viscera of wrinkled abalone and its application" (201510594885.7), although the preparation of anti-inflammatory peptide by simulating gastrointestinal digestion method, ignores the influence of small intestinal epithelial cells on its selective absorption

In summary, the current methods for preparing functional peptides from casein ignore the digestion and absorption of the gastrointestinal tract, and cannot truly simulate the digestion of bioactive peptides in the gastrointestinal tract and the absorption of peptides by small intestinal endothelial cells

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