Tissue culture and rapid propagation seedling culture method of paphiopedilum purpuratum

A technique for fast-growing seedlings and Venturi, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low seed germination rate, slow growth, low rooting rate of seedlings, etc. The effect of improving the germination rate, differentiation rate and bud height of seeds and improving the rooting rate of seedlings

Inactive Publication Date: 2018-05-04
QIANXINAN LVYUAN ANIMAL SCI & TECH DEV
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Problems solved by technology

[0003] The technical problem to be solved by the present invention is: to provide a method for tissue culture and rapid propagation of Paphiopedilum to solve the problems of low seed germination rate, low seedling rooting rate and slow growth of Paphiopedilum, and to speed up the development of Paphiopedilum. The growth and reproduction of orchid seedlings can meet the needs of large-scale planting of P.

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Embodiment Construction

[0011] The invention is further described below in conjunction with specific embodiments:

[0012] A method for rapid propagation of seedlings by tissue culture of Wenpiopsis, comprising the following steps:

[0013] 1. Disinfection treatment of P. chinensis seeds: the specific steps are: (1) first rinse with sterile water; (2) soak in 0.2% mercury liter for 15 minutes, and then rinse with sterile water; (3) finally rinse with 0.1 % hypochlorous acid soak for 5 minutes, then rinse with sterile water.

[0014] 2. Planting germination culture: the medium used for seed germination culture is 1 / 2MS+0.1mg / LKT+20 ml / L coconut milk+20g / L sucrose+6-7g / L agar powder, pH5.5, dark culture 30 -40 days, the culture temperature is 25±1°C.

[0015] 3. Protocorm culture: the medium used for protocorm culture is 1 / 2MS+0.2mg / LBA+0.3mg / L activated carbon+20g / L sucrose+20g / L banana+6-7g / L agar powder, PH5.5 , light culture 12hr / d, light intensity 1500-1200LUX, culture temperature 25±1℃.

[00...

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Abstract

The invention discloses a tissue culture and rapid propagation seedling culture method of paphiopedilum purpuratum. The method comprises the following steps: paphiopedilum purpuratum seed disinfection, seed germination culture, protocorm culture, rooting culture and test-tube seedling transplantation, wherein a medium for seed germination culture contains 1 / 2 MS, 0.1 mg / L KT, 20 ml / L coconut milk,20 g / L sucrose and 6-7 g / L agar powder, PH is 5.5, and dark culture is performed for 30-40 days at a temperature of 25 plus or minus 1 DEG C. With adoption of the technical scheme, the medium suitable for tissue culture of the paphiopedilum purpuratum is adopted for seed germination culture of the paphiopedilum purpuratum, and the germination rate of the paphiopedilum purpuratum is high; the coconut milk is added to the medium used in the stage and has a promotion effect on callus differentiation rate and shoot height of the paphiopedilum purpuratum, so that tissue cultured seedlings are thickened and strengthened, and seed germination rate and seedling rooting rate of the paphiopedilum purpuratum are increased.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of Paphiopedilum japonica, belonging to the technical field of tissue culture seedling cultivation. Background technique [0002] The plant of the genus Paphiopedilum is one of the most popular orchids with its peculiar flower shape and gorgeous flower color. In the past ten years, due to the destruction of the ecological environment and other reasons, many species have reached the verge of extinction. In order to alleviate the pressure on the collection of wild plants, protect endangered species, and meet people's needs for Paupelopera plants, scholars at home and abroad have conducted a lot of research on the artificial propagation of Paphiopedilum plants. At present, the aseptic sowing and tissue culture techniques of some Paphiopedilum species have been successful, but the germination rate of P. Artificial breeding. Therefore, it is of great significance to provide a meth...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor邓克云邓克兰刘森银周丽
OwnerQIANXINAN LVYUAN ANIMAL SCI & TECH DEV