Tissue-culture rapid propagation method for Cymbidium ensifolium varrubrigemmum

A tissue culture rapid propagation and subculture technology, applied in the field of plant tissue culture, can solve the problems of low value-added rate, long reproduction cycle, poor quality of tissue culture seedlings, etc., and achieve the effect of increasing disinfection.

Inactive Publication Date: 2018-05-04
GUANGXI YIMU AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since each orchid variety has its own characteristics in habit, when the explants of the four seasons orchid pedicel buds are used for tissue culture, the existing explant disinfection method and the common medium for tissue culture cannot be obtained. Satisfactory tissue culture results, resulting in long propagation cycle, low value-added rate, and poor quality of tissue culture seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for tissue culture rapid propagation of Sijilan, comprising disinfection of explants, induction culture, subculture and strong seedling culture, the specific steps are:

[0030] (1) Disinfection of explants: with the Sijilan which is blooming, growing vigorously and without damage by diseases and insect pests as the female parent, cut the pedicel buds on the pedicel of the female parent as the explants, first wash the pedicel buds with running water, and then use a volume concentration of Soak in 75% alcohol for 1min, then peel off the bracts on the surface of the pedicel buds, then soak in the plant disinfectant for 8min and continue to peel off one layer of bracts, then continue to soak in the plant disinfectant for 5min, use aseptic water 3 times to obtain sterilized explants; the plant disinfectant is made by mixing the following raw materials in parts by weight: 0.05 part of glycyrrhizic acid, 0.8 part of Radix isatidis extract, 0.5 part of Bupleuri extract...

Embodiment 2

[0035] A method for tissue culture rapid propagation of Sijilan, comprising disinfection of explants, induction culture, subculture and strong seedling culture, the specific steps are:

[0036] (1) Disinfection of explants: with the Sijilan which is blooming, growing vigorously and without damage by diseases and insect pests as the female parent, cut the pedicel buds on the pedicel of the female parent as the explants, first wash the pedicel buds with running water, and then use a volume concentration of Soak in 75% alcohol for 1min, then peel off the bracts on the surface of the pedicel buds, then soak in the plant disinfectant for 8min and continue to peel off one layer of bracts, then continue to soak in the plant disinfectant for 5min, use aseptic water 4 times to obtain the sterilized explants; the plant disinfectant is made by mixing the following raw materials in parts by weight: 0.06 part of glycyrrhizic acid, 1.0 part of Radix isatidis extract, 0.6 part of Bupleuri ext...

Embodiment 3

[0041] A method for tissue culture rapid propagation of Sijilan, comprising disinfection of explants, induction culture, subculture and strong seedling culture, the specific steps are:

[0042] (1) Disinfection of explants: with the Sijilan which is blooming, growing vigorously and without damage by diseases and insect pests as the female parent, cut the pedicel buds on the pedicel of the female parent as the explants, first wash the pedicel buds with running water, and then use a volume concentration of Soak in 75% alcohol for 2 minutes, then peel off the bracts on the surface of the pedicel buds, then soak in the plant disinfectant for 15 minutes and then continue to peel off one layer of bracts, then continue to soak in the plant disinfectant for 10 minutes, use sterile water 5 times to obtain the sterilized explant; the plant disinfectant is made by mixing the following raw materials in parts by weight: 0.08 parts of glycyrrhizic acid, 1.2 parts of Radix Radix Radix extract...

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PUM

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Abstract

The invention provides a tissue-culture rapid propagation method for Cymbidium ensifolium varrubrigemmum, belonging to the technical field of plant tissue culture. The method comprises an explant disinfection step, an inducing culture step, a subculture step, a strong seedling culture step and the like, wherein an explant is disinfected in an alcohol-plant disinfectant cooperated disinfection manner, and effective components of a plant disinfectant are glycyrrhizic acid, radix isatidis extract and a radix bupleuri extract; and horseradish tree leaf juice and folium mori juicea are added into culture mediums in each culture stage as nutrients. The method has the beneficial effects that the harm caused to the explant is small; and by utilizing a culture medium formula provided by the invention, the induced differentiation rate of Cymbidium ensifolium varrubrigemmum and the multiplication and rooting rate of the explant can be remarkably increased, the culture period can be shortened, andthe quality of tissue culture seedlings of Cymbidium ensifolium varrubrigemmum can be improved.

Description

【Technical field】 [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Four Seasons Orchid. 【Background technique】 [0002] Four Seasons Orchid is a kind of Chinese orchid. It is named because it can bloom in all seasons. It belongs to the ground orchid. It mainly blooms from July to August every year. It is mainly produced in Meishan City, Leshan City, Sichuan, Guangdong, Fujian, Taiwan and other places. Born on cliffs and in sparse forests, the locals call it Xialan. The plants of Sijilan are strong and strong, with thick and long roots. It is suitable to plant in orchid pots with more than five tubes, and the number of seedlings in each pot is a little more. When placed in the forest, garden or hall, the flowers and leaves are luxuriant and the momentum is great. You can also plant a few seedlings in a larger high-waisted pot for a long time. It is green and steep, very radiant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 张金显陈财宝
Owner GUANGXI YIMU AGRI TECH CO LTD
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