Method for detecting anti-inflammatory activity of herbal tea by use of expression level of ICAM-1 protein in human microvascular endothelial cells

A technology for ICAM-1 and protein expression, which is applied in the field of immune analysis, can solve the problems of not being able to quell inflammation in a timely and effective manner, and achieve the effect of authentic and reliable detection results, easy operation, and stable cell surface proteins

Inactive Publication Date: 2018-05-11
HUAZHONG AGRI UNIV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The problem with inflammation is not that it starts smoothly, but that when the threat is ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting anti-inflammatory activity of herbal tea by use of expression level of ICAM-1 protein in human microvascular endothelial cells
  • Method for detecting anti-inflammatory activity of herbal tea by use of expression level of ICAM-1 protein in human microvascular endothelial cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 LPS induces the expression of ICAM-1 protein in HMEC-1 cells after incubation with different concentrations of herbal tea

[0032] Preparation of herbal tea test solution:

[0033] In weight / volume (mg / L):

[0034] (1) Weigh 10g of Chinese herbal medicine frangipani, 10g of honeysuckle, 10g of chrysanthemum, 20g of cloth dregs leaves, 20g of licorice, and 10g of Prunella vulgaris. Kit Kat Trading Co., Ltd.) decocting time is 2 hours in total (high heat, for example, electric decoction pot set power is 450W, that is, 100 ℃, decoction 1h; warm fire, such as electric decoction pot is set power is 300W, that is, 85 ~ 95 ℃ decoct for 1 h), repeat the decoction once, filter with qualitative filter paper (purchased from Hangzhou Wohua Filter Paper Co., Ltd.), respectively, combine the two filtrates, and collect the filtrate after vacuum filtration;

[0035] (2) Concentrate the filtrate obtained in step (1) by rotary evaporation at -0.1MPa and 65°C respectively to f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention relates to a method for detecting anti-inflammatory activity of herbal tea by use of expression level of ICAM-1 (intercellular adhesion molecule-1) protein in human microvascularendothelial cells. The ICAM-1 protein on the surface of the HMEC-1 cells is directly labeled with a fluorescent antibody without extracting of RNA, the expression level of the ICAM-1 is detected by aflow cytometry, specifically, the HMEC-1 cells are inoculated in a cell culture plate, after the cells grow to form a single layer, the cell culture plate is incubated with a herbal tea test solutionand the cells, then the HMEC-1 cells are treated with LPS to induce inflammation, the expression level of the ICAM-1 on the surface of the inflammatory HMEC-1 cells is significantly increased, and after the cells are incubated with the herbal tea test solution, the expression level of the ICAM-1 is suppressed. The ICAM-1 protein on the surface of the HMEC-1 cells is labeled with the fluorescencelabeling ICAM-1 antibody, the expression level of the ICAM-1 on the surface of the inflammatory HMEC-1 cells incubated with the herbal tea test solution is quantitatively detected by the flow cytometry, and the expression level of the ICAM-1 protein is used as an index for measuring of the anti-inflammatory effect of the herbal tea test solution.

Description

technical field [0001] The invention belongs to the technical field of immune analysis, and in particular relates to a method for detecting the anti-inflammatory activity of herbal tea by using the expression level of intercellular adhesion factor (ICAM-1) protein on the surface of human microvascular endothelial cells (HMEC-1). More specifically, at the cellular level, the herbal tea was first incubated with HMEC-1 cells, and then lipopolysaccharide (LPS) was used to induce HMEC-1 cells to produce an inflammatory response, that is, LPS induced HMEC-1 cell surface ICAM-1 protein high However, after pretreatment with different herbal tea test solutions, the expression level of ICAM-1 protein molecules in HMEC-1 cells will be inhibited to varying degrees. Level of inhibition rate, quantitative analysis of anti-inflammatory effect of herbal tea. Background technique [0002] Inflammation is an instinctive and necessary response of the body in response to pathogenic infection, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/02G01N33/68G01N33/577G01N33/533
CPCG01N33/5008G01N33/5064G01N33/533G01N33/577G01N33/68G01N2333/47
Inventor 胡学博王嘉男
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap