DPO (Dual Priming Oligonucleotide) primer group for porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus and porcine rotavirus detection and application of DPO primer group

Abstract

The invention discloses a DPO (Dual Priming Oligonucleotide) primer group for porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus and porcine rotavirus detection and application of the DPO primer group. Aiming to a TGEV-N gene, a PEDV-N gene and a PRoV-VP7 gene, a pair of DPO primers respectively, and a multiplex DPO real-time RT-PCR detection method of a porcine epidemicdiarrhea virus, a porcine transmissible gastroenteritis virus and a porcine rotavirus is established. Results show that a detection limit of the method is 5.3*100 copies/microlitre, target gene fragments can be efficiently amplified within an annealing temperature range from 40 DEG C to 65 DEG C, and shown that the method is wide in annealing temperature range; and meanwhile, the DPO primers are strong in specificity, and no non-specific amplification is generated in the PCR process. The multiplex DPO real-time RT-PCR detection method provided by the invention is simple to design, strong in specificity and high in sensitivity and provides a new technological means to rapid and accurate detection of three porcine viral diarrhea infective pathogens of TGEV, PEDV and PRoV.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to View More

AI Technical Summary

Benefits of technology

This patented technology uses two different techniques - one involves combining certain chemical substances together into small molecules that form tiny particles called DNA probes or dots (DNA) which when exposed to light activate genetic material within cells causing them to produce various types of signals such as changes in pH levels inside their body tissues. These signals help detect diseases caused by these microorganisms.

Problems solved by technology

This patents describes different techniques or diagnostic tools currently available but each technique suffers from various drawbacks such as low precision, time consumption, complexity, difficulty in identifying certain types of bacteria with varying degrees of severity, limited ability to detect many disease agents simultaneously, lack of sufficient sensitiveness towards common strains found among all three species, etc., making current testing procedures challenged because there may still remain some cases where no effective treatment protocol exists.

Images