Microfluidic chip with microstructure array and nucleic acid solid phase purification and extraction method

Abstract

The invention discloses a microfluidic chip with a microstructure array and a nucleic acid solid phase purification and extraction method. The microfluidic chip includes a microfluidic channel and a microstructure array distributed in the microfluidic channel. The microstructure array comprises a plurality of micropillars arranged in an array. The surfaces of the micropillars are connected to an adsorbent. The adsorbent is used for adsorbing nucleic acid. Compared with the prior art, the method utilizes the microfluidic chip with a microstructure array as a base, realizes adsorption and elution of nucleic acid through modification of the microfluidic channel interior with an agent for physical absorption of nucleic acid and has simple, fast and efficient operation processes. The microfluidic chip is easily integrated with a downstream nucleic acid amplification microfluidic chip so that a chip lab is constructed and is especially for on-site rapid detection.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic chip containing a microarray structure and a nucleic acid solid-phase extraction method based on the chip, belonging to the field of solid-phase extraction. Background technique [0002] Biomolecular nucleic acid extraction technology is the basis for all aspects of molecular biology research and a key technology for life science research and application. The extraction of nucleic acid mainly refers to separating it from biological macromolecules such as proteins, polysaccharides, and lipids, and ensuring the integrity of nucleic acid molecules. In the early 1990s, nucleic acid extraction and purification technology was still a time-consuming and cumbersome technology, and at the same time, the use of toxic reagents was required. With the emergence of solid-phase extraction technology and the effectiveness and practicability of commercial kits, it has helped the rapid and re...

AI Technical Summary

Problems solved by technology

Traditional nucleic acid extraction methods have many disadvantages, such as: complex and cumbersome procedures, time-consuming, high cost, low extraction efficiency, and the need to use reagents that inhibit nucleic acid amplification reactions, etc.

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