Microfluidic chip with microstructure array and nucleic acid solid phase purification and extraction method

A technology of microfluidic chips and microstructure arrays, applied in biochemical equipment and methods, biochemical instruments, biochemical cleaning devices, etc., can solve the problems of complex and cumbersome procedures, low extraction efficiency, and high cost, and achieve simple operation procedures, Low cost, low cost effect

Pending Publication Date: 2018-06-01
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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Problems solved by technology

Traditional nucleic acid extraction methods have many disadvantages, such as: complex and cumbersome procedures, time-consuming, high cost, low extraction efficiency, and the need to use reagents that inhibit nucleic acid amplification reactions, etc.

Method used

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  • Microfluidic chip with microstructure array and nucleic acid solid phase purification and extraction method
  • Microfluidic chip with microstructure array and nucleic acid solid phase purification and extraction method
  • Microfluidic chip with microstructure array and nucleic acid solid phase purification and extraction method

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Embodiment Construction

[0021] As mentioned above, the prior art lacks a universal nucleic acid solid-phase extraction technology that is easy to integrate with microfluidic chips such as downstream nucleic acid amplification to build a lab-on-a-chip. For this reason, the present invention provides a microarray structure Fluidic chip and nucleic acid solid-phase extraction method based on the chip, which can meet various factors, such as: the effectiveness of equipment, the balance between reagents and samples and flow rate, and the extraction efficiency and quality of nucleic acid, etc., especially suitable for Rapid detection on site.

[0022] Further, a microfluidic chip with a microstructure array provided by an embodiment of the present invention includes a microfluidic channel and a microstructure array distributed in the microfluidic channel, and the microstructure array includes a plurality of A microcolumn, wherein the surface of the plurality of microcolumns is also connected with an adsorb...

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Abstract

The invention discloses a microfluidic chip with a microstructure array and a nucleic acid solid phase purification and extraction method. The microfluidic chip includes a microfluidic channel and a microstructure array distributed in the microfluidic channel. The microstructure array comprises a plurality of micropillars arranged in an array. The surfaces of the micropillars are connected to an adsorbent. The adsorbent is used for adsorbing nucleic acid. Compared with the prior art, the method utilizes the microfluidic chip with a microstructure array as a base, realizes adsorption and elution of nucleic acid through modification of the microfluidic channel interior with an agent for physical absorption of nucleic acid and has simple, fast and efficient operation processes. The microfluidic chip is easily integrated with a downstream nucleic acid amplification microfluidic chip so that a chip lab is constructed and is especially for on-site rapid detection.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic chip containing a microarray structure and a nucleic acid solid-phase extraction method based on the chip, belonging to the field of solid-phase extraction. Background technique [0002] Biomolecular nucleic acid extraction technology is the basis for all aspects of molecular biology research and a key technology for life science research and application. The extraction of nucleic acid mainly refers to separating it from biological macromolecules such as proteins, polysaccharides, and lipids, and ensuring the integrity of nucleic acid molecules. In the early 1990s, nucleic acid extraction and purification technology was still a time-consuming and cumbersome technology, and at the same time, the use of toxic reagents was required. With the emergence of solid-phase extraction technology and the effectiveness and practicability of commercial kits, it has helped the rapid and re...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N15/10
CPCC12N15/1006
Inventor 卢洁王宏
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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