Analysis method of cyclic adenosine monophosphate in red jujubes

A central adenosine monophosphate and analysis method technology, which is applied to the analysis of materials, material separation, measurement devices, etc., can solve the problems of poor anti-matrix interference, cumbersome operation, and long time, and achieve strong anti-matrix interference and short analysis time. Short, good separation effect

Inactive Publication Date: 2018-06-12
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, paper chromatography, thin-layer chromatography, protein binding assay, radioimmunoassay and other methods are cumbersome to operate, take a long time, a

Method used

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  • Analysis method of cyclic adenosine monophosphate in red jujubes
  • Analysis method of cyclic adenosine monophosphate in red jujubes

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0020] Example 1

[0021] A method for analyzing cyclic adenosine monophosphate in jujube includes the following steps:

[0022] Step 1. Quantitative working curve

[0023] Take an appropriate amount of cAMP reference substance, accurately weigh it, add ultrapure water to prepare a mixed stock solution of 1.00mg / mL, and store it at 4°C; dilute with ultrapure water when used, and prepare cAMP with a mass concentration of 0.010, 1.00, 10.00 , 50.00, 100.0, 500.0, 1000.0ng / mL mixed reference solution;

[0024] Step 2. Chromatographic conditions

[0025] ZORBAX SB-Aq C18 chromatographic column with specifications of 3.5um, 4.6mm x 150mm, column temperature of 20℃, mobile phase of water-acetonitrile 40:60, flow rate of 0.5mL / min, and injection volume of 4uL;

[0026] Step 3. Mass spectrometry conditions

[0027] Using ESI, positive ion detection, atomizing gas N 2 The pressure is set to 0.27MPa; dry gas N 2 The flow rate is set to 9L / min, dry gas N 2 The temperature is set to 340℃; the mass-t...

Example Embodiment

[0028] Example 2

[0029] A method for analyzing cyclic adenosine monophosphate in jujube includes the following steps:

[0030] Step 1. Quantitative working curve

[0031] Take an appropriate amount of cAMP reference substance, accurately weigh it, add ultrapure water to prepare a mixed stock solution of 1.00mg / mL, and store it at 4°C; dilute with ultrapure water when used, and prepare cAMP with a mass concentration of 0.010, 1.00, 10.00 , 50.00, 100.0, 500.0, 1000.0ng / mL mixed reference solution;

[0032] Step 2. Chromatographic conditions

[0033] ZORBAX SB-Aq C18 chromatographic column with specifications of 3.5um, 4.6mm x 150mm, column temperature of 30℃, mobile phase of water-acetonitrile 40:55, flow rate of 0.6mL / min, injection volume of 5uL;

[0034] Step 3. Mass spectrometry conditions

[0035] Using ESI, positive ion detection, atomizing gas N 2 The pressure is set to 0.28MPa; dry gas N 2 The flow rate is set to 10L / min, dry gas N 2 The temperature is set to 350℃; the mass-to-c...

Example Embodiment

[0036] Example 3

[0037] A method for analyzing cyclic adenosine monophosphate in jujube includes the following steps:

[0038] Step 1. Quantitative working curve

[0039] Take an appropriate amount of cAMP reference substance, accurately weigh it, add ultrapure water to prepare a mixed stock solution of 1.00mg / mL, and store it at 4°C; dilute with ultrapure water when used, and prepare cAMP with a mass concentration of 0.010, 1.00, 10.00 , 50.00, 100.0, 500.0, 1000.0ng / mL mixed reference solution;

[0040] Step 2. Chromatographic conditions

[0041] ZORBAX SB-Aq C18 chromatographic column with specifications of 3.5um, 4.6mm x 150mm, column temperature of 40℃, mobile phase of water-acetonitrile 40:50, flow rate of 0.7mL / min, injection volume of 6uL;

[0042] Step 3. Mass spectrometry conditions

[0043] Using ESI, positive ion detection, atomizing gas N 2 The pressure is set to 0.29MPa; dry gas N 2 The flow rate is set to 11L / min, dry gas N 2 The temperature is set to 360°C; the mass-to-...

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Abstract

The invention discloses an analysis method of cyclic adenosine monophosphate in red jujubes. The method comprises the following steps: step 1, establishment of a quantitative working curve; step 2, establishment of chromatographic conditions; and step 3, establishment of mass spectrometry conditions. According to Tthe invention, establishes a high performance liquid chromatography-tandem mass spectrometry method is established for measuring cyclic adenosine monophosphate, and the method has the characteristics of short analysis time, good separation degree, high sensitivity, strong anti-matrixinterference capability and simple and convenient operation. In addition, the high performance liquid chromatography-tandem mass spectrometry method can provide accurate molecular weight informationof compound parent ions and daughter ions, can conclude a molecular formula, is very suitable for structural analysis and discrimination of compounds, and provides a scientific research and analysis method for researching the content of cyclic adenosine monophosphate in vivo at the later period.

Description

Technical field [0001] The invention belongs to the technical field of chemistry and relates to a method for analyzing cyclic adenosine phosphate in red jujube. Background technique [0002] Since the discovery of cyclic adenosine monophosphate, thousands of laboratories around the world have been studying this magical molecule. Scientists have discovered that most biological species on the earth contain cyclic adenosine phosphate, but like other signal substances, its content is very small and difficult to extract. Only a very small amount of cyclic phosphate can be extracted from animal livers. Adenosine. In the past, only chemically synthesized pharmaceutical grade cyclic adenosine monophosphate products for intravenous injection were available in the market. The output was small and the price was much higher than that of gold. In 1979 and 1984, two Japanese scientists discovered that China's unique jujube fruit is rich in cyclic adenosine monophosphate, which is tens of tho...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 吴洪斌党富民杨慧贾文婷代文婷金新文吴宏王俊钢刘成江李宇辉鲁立良郭安民张强雷用东宋方圆
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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