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A breeding method for reducing cadmium content in rice grains

A rice grain and content technology, applied in the field of plant ecology and agriculture, can solve the problems of unspecified site mutation, inability to realize mutants, inability to specify gene silencing, etc., to avoid potential safety hazards, retain excellent characteristics, and shorten breeding time the effect of time

Active Publication Date: 2021-07-30
HUNAN UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the mutants obtained by these methods are random and uncontrollable, cannot silence specific genes, cannot mutate specific sites, and cannot achieve gene knockout mutants, so gene editing technology has practical significance for rice production major

Method used

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  • A breeding method for reducing cadmium content in rice grains
  • A breeding method for reducing cadmium content in rice grains
  • A breeding method for reducing cadmium content in rice grains

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Embodiment 1

[0050] In the present embodiment, a kind of breeding method that reduces cadmium content of rice grain specifically comprises the following steps:

[0051] (1) Cloning the full length of the exon of OsNCX8 of the rice receptor material, and sequencing;

[0052] Design specific primer sequences to clone the full length of the exon of OsNCX8 of the rice receptor material and sequence; the primer sequence is: F1: 5'-ATGGCGCTCCTGCGCAGGCGG-3', which is shown in SEQ ID NO: 2; R1: 5' -CTATGCTGGCCACCAGGAGT-3', which is represented by SEQ ID NO:3. Cloning and sequencing obtained the full-length sequence of the NCX8 gene of different rice varieties such as Nipponbare, 9311, and Takata. Sequencing found that the gene sequence had no base difference, indicating that the gene was highly conserved during the evolution process. The full-length sequence of the gene is as follows. Shown as SEQ ID NO:1:

[0053] ATGGCGCTCCTGCGCAGGCGGGGGTACAGTGCCGCCGTCCCCAGCGCCTGCTGCTTCCTCCTCCTGCTGCTCGTCCTCTCC...

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Abstract

The invention discloses a breeding method for reducing the cadmium content of rice grains. CRISPER / Cas9 technology is used to knock out the rice OsNCX8 gene in a targeted manner to obtain transgenic plants knocked out of the OsNCX8 gene, from which a homozygous plant with complete inactivation of the OsNCX8 protein is selected to finally obtain Plants without genetically modified ingredients and reduced cadmium content in rice. The OsNCX8 protein is the first member of the sodium-calcium exchange protein family identified in rice, and the inactivation of the OsNCX8 protein leads to a reduction of about 45% in grain cadmium content. Moreover, the agronomic traits of plants with OsNCX8 protein inactivation were not significantly changed compared with normal plants. at T 0 Homozygous plants with complete inactivation of the OsNCX8 protein can be obtained from the offspring plants, which greatly shortens the breeding time, and plants without transgenic components can be screened from their offspring, avoiding the safety hazards caused by transgenes.

Description

technical field [0001] The invention relates to the fields of plant ecology and agricultural technology, in particular to a breeding method for reducing the cadmium content of rice grains. Background technique [0002] Rice is one of the main food crops in my country. With the development of industry and agriculture, heavy metals in the environment and soil, especially heavy metal cadmium, accumulate in various tissues of rice, affecting the yield and quality of rice. Cadmium cannot be degraded in the environment and can seriously endanger human health through the food chain. At present, the heavy metal pollution of rice in Hunan Province is serious, especially the heavy metal cadmium content in the grain (rice) exceeds the standard, which threatens human health. It is very important to reduce the cadmium content in the grain. The toxicity of cadmium to rice can be alleviated and the cadmium content in rice can be reduced by measures such as changing soil, optimizing water ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29C12N15/113A01H5/10A01H6/46
CPCC07K14/415C12N15/8213C12N15/8243
Inventor 郝小花田连福李东屏陈良碧
Owner HUNAN UNIV OF ARTS & SCI
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