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Guanzhuang spotted pig SNP site, SNP chip and detection primer combination thereof, detection kit and application and germplasm identification method thereof

A detection kit and detection primer technology are applied in the fields of animal molecular biotechnology and animal genetics and breeding, which can solve the problems of rare Guanzhuang flower pigs, research on the germplasm characteristics of Guanzhuang flower pigs, etc., and achieve good development and application prospects. , Good practical promotion and application value, strong practical effect

Active Publication Date: 2018-07-27
上杭傲农槐猪产业发展有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, there are not many studies on Guanzhuang Huapig, especially the research on molecules and SNPs needs to be improved; at present, there is no research on the germplasm characteristics of Guanzhuang Huapig. in-depth

Method used

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  • Guanzhuang spotted pig SNP site, SNP chip and detection primer combination thereof, detection kit and application and germplasm identification method thereof
  • Guanzhuang spotted pig SNP site, SNP chip and detection primer combination thereof, detection kit and application and germplasm identification method thereof
  • Guanzhuang spotted pig SNP site, SNP chip and detection primer combination thereof, detection kit and application and germplasm identification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0156] This embodiment provides a Guanzhuang Huapig SNP site, and the Guanzhuang Huapig SNP site includes:

[0157] (1) MARC0110659, SNP locus genotype: AA;

[0158] (2) H3GA0003731, SNP site genotype: GG;

[0159] (3) DRGA0002305, SNP site genotype: AA;

[0160] (4) ASGA0009835, SNP site genotype: GG;

[0161] (5) MARC0113860, SNP site genotype: TT;

[0162] (6) ASGA0020113, SNP site genotype: AA;

[0163] (7) ALGA0028821, SNP site genotype: TT;

[0164] (8) DRGA0005669, SNP site genotype: GG;

[0165] (9) DRGA0005851, SNP site genotype: AA;

[0166] (10) MARC0021061, SNP site genotype: TT;

[0167] (11) ALGA0050422, SNP site genotype: AA;

[0168] (12) MARC0020221, SNP locus genotype: AA;

[0169] (13) ALGA0070475, SNP site genotype: TT;

[0170] (14) MARC0109383, SNP site genotype: AA;

[0171] (15) MARC0049346, SNP site genotype: AA;

[0172] (16) ASGA0064559, SNP site genotype: CC;

[0173] (17) ALGA0086332, SNP site genotype: CC;

[0174] (18) MARC0071437, ...

Embodiment 2

[0179] This example provides a detection primer combination for detecting the SNP loci of Guanzhuang Huapig provided in Example 1. The detection primer combination includes corresponding detection SNP loci of MARC0110659, SNP loci of H3GA0003731, SNP loci of DRGA0002305, ASGA0009835 SNP site, SNP site of MARC0113860, SNP site of ASGA0020113, SNP site of ALGA0028821, SNP site of DRGA0005669, SNP site of DRGA0005851, SNP site of MARC0021061, SNP site of ALGA0050422, SNP site of MARC0020221 point, the SNP site of ALGA0070475, the SNP site of MARC0109383, the SNP site of MARC0049346, the SNP site of ASGA0064559, the SNP site of ALGA0086332, the SNP site of MARC0071437 and the first primer pair of the SNP site of H3GA0046776 Nineteen primer pairs, the base sequences of the first primer pair to the nineteenth primer pair are shown in the sequence SEQ ID NO.1-38.

[0180] Since the above combination of detection primers is mainly aimed at the SNP site provided in Example 1, the above...

Embodiment 3

[0182] This embodiment provides a detection kit for the detection of SNP sites in Guanzhuang Huapig. SNP site of DRGA0002305, SNP site of ASGA0009835, SNP site of MARC0113860, SNP site of ASGA0020113, SNP site of ALGA0028821, SNP site of DRGA0005669, SNP site of DRGA0005851, SNP site of MARC0021061 0504 of AL2 SNP site, SNP site of MARC0020221, SNP site of ALGA0070475, SNP site of MARC0109383, SNP site of MARC0049346, SNP site of ASGA0064559, SNP site of ALGA0086332, SNP site of MARC0071437 and SNP site of H3GA0046776 Point the first primer pair to the nineteenth primer pair, the base sequence of the first primer pair to the nineteenth primer pair is shown in the sequence SEQ ID NO.1-38.

[0183] Of course, the detection kit can also have related reagents for performing PCR reaction, including at least one of PCR reaction buffer, TaqDNA polymerase and dNTPs.

[0184] Of course, nucleic acid extraction reagents may also be included.

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Abstract

The invention provides a Guanzhuang spotted pig SNP site and an establishment method and an application thereof, and belongs to the field of the animal molecular biological technology and animal genetic breeding. The provided Guanzhuang spotted pig SNP site has the apparent species specificity of the Guanzhuang spotted pig, and has the better development and application prospect, and is stronger in practicability. The Guanzhuang spotted pig SNP site and a detection primer for detecting the SNP site are used for preparing a SNP chip or a detection kit, and the SNP chip or the detection kit is used for the germplasm identification of the Guanzhuang spotted pig, a better detection result is obtained, and better data support is provided for the germplasm identification. The Guanzhuang spottedpig SNP site has the better social value and practical popularization and application value.

Description

technical field [0001] The invention relates to the field of animal molecular biotechnology and animal genetic breeding, in particular to the SNP site of Guanzhuang Hua pig, the SNP chip and its combination of detection primers, a detection kit and its application, and a germplasm identification method. Background technique [0002] my country has a vast territory and abundant resources. It is also one of the earliest countries in the world to raise pigs. It has a history of thousands of years. Pigs are known as the first of the six animals in China. Due to the influence of natural environment, economic conditions and folk culture, my country has abundant and unique local pig breed resources, which are valuable resources in the world pig breed gene bank. [0003] Guanzhuang Huapig Guanzhuang Huapig is one of the eight local excellent pig breeds in Fujian Province. The local pig breeds have strong adaptability, docile temperament, tolerance to coarseness and easy raising, str...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 龙毅黄立林万文峰丁能水吴有林
Owner 上杭傲农槐猪产业发展有限公司
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