Marker for glioblastoma, application thereof and kit
A technology for glioblastoma and glioma, applied in the field of tissue tumor markers
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Embodiment 1
[0021] Embodiment 1: Through the data analysis of TCGA database, mine the relationship between METTL3 gene expression and the survival rate of glioblastoma patients.
[0022] The TCGA standard method was used to download the expression profile microarray data of two groups of glioblastoma tissue samples and the survival status of clinical follow-up patients, and statistical software was used to draw the relationship between the expression level of METTL3 and the overall survival rate of glioblastoma patients.
[0023] Result: see figure 1 , the expression of METTL3 was significantly correlated with the survival rate of glioblastoma patients, and the overall survival rate of patients with high expression of METTL3 was significantly lower than that of patients with low expression of METTL3, suggesting that METTL3 may play a role in promoting cancer development.
Embodiment 2
[0024] Example 2: mining the relationship between the expression of METTL3 and the overall survival rate of glioma patients through the database.
[0025] The TCGA database was used to download the transcriptome RNA-seq sequencing data of glioma patients, and the expression level of METTL3 in different samples was analyzed.
[0026] Result: see figure 2 A, analysis found that compared with normal brain tissue, the expression level of METTL3 in glioma was significantly increased, which can be considered as an early diagnostic indicator for glioma.
Embodiment 3
[0027] Example 3: RT-qPCR verification of the expression difference of METTL3 gene in glioma samples and normal brain tissues.
[0028] By measuring the METTL3 gene expression status in normal brain tissue cells and tumor tissues of patients with glioma, 10 tissue samples in the control group were obtained from patients with traumatic brain tissue. Glioma tissue was obtained through neurosurgical resection and confirmed by pathological diagnosis.
[0029] First, total cellular RNA was extracted. Keep all operations and related reagents on ice. Use Trizol reagent to extract total RNA from cells, and the specific steps are as follows: Prepare to extract total RNA. Cells were directly digested and lysed with Trizol, and Trizol was added. After the cells were added with Trizol, they were left at room temperature for 5 min to fully lyse. Use a high-speed refrigerated centrifuge to centrifuge at 12,000 rpm at 4°C for 5 minutes, and discard the precipitate. Add chloroform at 200u...
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