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Acellular dermal matrix guided tissue regeneration membrane material as well as preparation method and application thereof

A technology that guides tissue regeneration and decellularized dermis, applied in tissue regeneration, medical science, prostheses, etc., to achieve the effect of increasing production volume, increasing migration speed, and convenient operation

Active Publication Date: 2018-08-03
QINGDAO MARINE BIOPHARMACEUTICAL RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention proposes an acellular dermal matrix guiding tissue regeneration membrane material and its preparation method and application, which solves the problem of carrying and spreading risks of zoonotic viruses in the guiding tissue regeneration membrane in the prior art

Method used

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  • Acellular dermal matrix guided tissue regeneration membrane material as well as preparation method and application thereof
  • Acellular dermal matrix guided tissue regeneration membrane material as well as preparation method and application thereof
  • Acellular dermal matrix guided tissue regeneration membrane material as well as preparation method and application thereof

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preparation example Construction

[0032] A preparation method of the acellular dermal matrix-guided tissue regeneration membrane material of the present invention comprises the following steps:

[0033] 1) Pre-treatment: take fish skin, remove fish scales, inner fat and excess fish meat;

[0034] 2) Washing: The fish skin obtained in step 1) is repeatedly rinsed several times with physiological saline, and then, repeatedly rinsed several times with ultrapure water;

[0035] 3) Remove the epidermis and prepare loose noodles: place the tissue obtained in step 1) in a 0.01-1.0mol / L acid solution, shake at 20-37°C for 5-30 minutes, to remove the epidermis and obtain loose noodles ;

[0036] 4) Stabilization: place the tissue obtained in step 3) in a 0.5-2.5 mol / L sodium chloride solution, and shake for 30-120 min at 20-37°C to stabilize the structure;

[0037] 5) Decellularization: freeze-thaw the tissue obtained in step 4) at -80 to -70°C, freeze and thaw several times, and then use 0.5 to 4.0% sodium deoxychol...

Embodiment 1

[0050] A preparation method of the acellular dermal matrix-guided tissue regeneration membrane material of the present invention comprises the following steps:

[0051] 1) Pre-treatment: remove the fish skin from the fish, and remove the fish scales, inner fat and excess fish meat with a knife;

[0052] 2) Washing: The fish skin obtained in step 1) was repeatedly washed with physiological saline for 3 times, each time lasting 10 minutes, and then repeatedly washed with ultrapure water for 3 times, each time lasting 10 minutes;

[0053] 3) Remove the epidermis and prepare loose noodles: put the material obtained in step 2) into 0.01mol / L formic acid solution, and shake for 30 minutes in a constant temperature shaker at 20°C to remove the epidermis and prepare loose noodles;

[0054] 4) Stabilization: put the material obtained in step 3) into a 0.5mol / L sodium chloride solution, and shake for 120 minutes in a constant temperature shaker at 20°C to stabilize the structure;

[00...

Embodiment 2

[0061] A preparation method of the acellular dermal matrix-guided tissue regeneration membrane material of the present invention comprises the following steps:

[0062] 1) Pre-treatment: remove the fish skin from the fish, and remove the fish scales, inner fat and excess fish meat with tweezers;

[0063] 2) Washing: the fish skin obtained in step 1) was repeatedly washed with physiological saline for 5 times, each time lasting 15 minutes, and then repeatedly washed with ultrapure water for 5 times, each time lasting 15 minutes;

[0064] 3) Remove the epidermis and prepare loose noodles: put the material obtained in step 2) into 0.1mol / L hydrochloric acid solution, shake for 10 minutes in a constant temperature shaker at 25°C, remove the epidermis and prepare loose noodles;

[0065] 4) Stabilization: put the material obtained in step 3) into a 1.0mol / L sodium chloride solution, and shake for 90 minutes in a constant temperature shaker at 37°C to stabilize the structure;

[006...

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Abstract

The invention provides an acellular dermal matrix guided tissue regeneration membrane material as well as a preparation method and application thereof, and relates to the technical field of medical biological materials. The preparation method provided by the invention comprises the following steps: pretreating, removing an epidermal layer, preparing a loose surface, stabilizing, performing the accellular treatment, washing, and sterilizing. A membrane material prepared by the method provided by the invention adopts fish skin as a raw material and has a three-dimensional space framework structure, and comprises a compact surface formed by a biological substrate membrane and a collagen loose surface formed by a dermal scaffold. By adopting the membrane material provided by the invention, therisk of the existing tissue restoration material in carrying and propagating the human-animal viruses can be avoided, the cell components can be completely removed, the immunogenicity is low, the clearance of the collagen fibers is increased, a corium layer structure is reserved, the tissue compatibility is good, the mechanical shielding effect is good, and the generation of new bones can be facilitated; and when used for restoring and treating the bone deficiency of an oral implantation operation, the material is digestible, and the second operation and the injury of the membrane material topatients can be effectively avoided.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to an acellular dermal matrix guided tissue regeneration membrane material and its preparation method and application. Background technique [0002] In recent years, with the continuous development and progress of the level of science and technology, the emergence of a new repair technology—membrane guided bone / tissue regeneration (MGBR / MGTR) has greatly improved the level of oral restorative science. . Guided bone / tissue regeneration (GBR / GTR) has the function of guiding osteogenesis and promoting the formation of osseointegration around the implant. Cells are faster than cells forming alveolar bone. Biomaterials are used to make a biofilm with a dense surface and a loose surface. The dense surface acts as a barrier to prevent fibroblasts and epithelial cells in soft tissues from growing into the bone defect area, avoiding these The cells compete with the cells capab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/58
CPCA61L27/3633A61L27/365A61L27/3687A61L27/3691A61L27/58A61L2430/02A61L2430/40
Inventor 王园园李八方
Owner QINGDAO MARINE BIOPHARMACEUTICAL RES INST
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