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Application of phellinus igniarius aqueous extract in trioxypurine resistance

A technology of water extract and phellinus, which is applied in the field of phellinus extract and its application, can solve the problems of scarcity, harsh conditions, and difficulty in realizing large-scale cultivation, and achieve the effect of treating gout and reducing uric acid deposition

Pending Publication Date: 2018-08-10
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phellinus Phellinus only grows on live mulberry trees. In view of the special physiological state of only growing on live mulberry trees and the constraints of the external environment, the fruiting bodies of Phellinus Phellinus grow slowly and are relatively rare in nature. Although it can be cultivated artificially, the The conditions are harsh, and the efficiency of obtaining is low. It is difficult to realize large-scale cultivation at present.

Method used

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  • Application of phellinus igniarius aqueous extract in trioxypurine resistance
  • Application of phellinus igniarius aqueous extract in trioxypurine resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the preparation of Phellinus Phellinus aqueous extract

[0020] (1) get Phellinus Phellinus growing in Changbai Mountain area and break into powder;

[0021] (2) Weigh a certain amount of Phellinus powder, add 10 times the volume of water according to the ratio of 1:10 (m / v), stir evenly, put it in a boiling water bath and cook for 2 hours to obtain a mixed solution.

[0022] (3) Take out the mixed solution and cool it down, ultracentrifuge at 4800rpm / min for 15min, and take the supernatant.

[0023] (4) Filter the supernatant to obtain Phellinus Phellinus water extract, autoclave at 121° C. for 30 minutes, and store in a refrigerator at 4° C. for later use.

[0024] During the detection, add the Phellinus radix alcohol extract obtained in step 5 into a mass spectrometer, and detect the types and contents of the substances contained in the Phellinus radix alcohol extract by MALDI-TOF time-of-flight mass spectrometry. The detection results are shown in fi...

Embodiment 2

[0025] Example 2: Establishment of Rat Hyperuricemia Model

[0026] (1) 8 Wistar rats aged 7-8 weeks were divided into groups according to blank group (Control), model group (Model), Phellinus igniarius water extract group (Phelinus igniarius), and colchicine positive group (Colchicine). Group 2 mice.

[0027] (2) Before the establishment of the model, the blank group and the model group were fed with ultrapure water, and the Phellinus water extract group and the colchicine group were fed with the Phellinus water extract and colchicine respectively, and each group was continuously fed for 9 days , orally administered once a day, 2 mL each time.

[0028] (3) Modeling was done on the 6th day of gavage. Rats in the blank group were injected with 100 μL of normal saline into the joint cavity, and 100 μL of sodium urate solution with a concentration of 30 mg / mL were injected into the model group, the Phellinus Phellinus water extract group and the colchicine group.

Embodiment 3

[0029] Embodiment 3: the mensuration of rat joint swelling rate

[0030] At the 0h, 4h, 12h, 24h, 48h, 72h and 96h of the modeling, the circumference of the rat joints was measured, and the swelling rate of the joints was calculated. [Swelling ratio=(S 1 -S 0 ) / S 0 ;(S 1 is the circumference at any point in time, S 0 is the circumference at 0h)].

[0031] From figure 2 It can be seen that in 0-24h, the joint swelling rate of the rats is on the rise, and the joint swelling rate of the 24-96h rats is on the decline. The joint swelling rate of the rats in the group was smaller than that in the other groups, indicating that Phellinus Phellinus water extract can significantly reduce the deposition of uric acid in the joint cavity.

[0032]The invention utilizes water to extract the active ingredients of Phellinus Phellinus, and feeds rats in the form of gavage. The local high uric acid model was established by injecting sodium urate into the joint cavity, and the water ext...

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Abstract

The invention relates to application of a phellinus igniarius aqueous extract in trioxypurine resistance. The phellinus igniarius aqueous extract mainly comprises two substances with mass-to-charge ratio of 1015.0270 and 1029.2048 daltons; the spectral strength of the two substances in mass spectrometric detection is 60% and 100%. A phellinus igniarius alcohol is prepared according to the following method: crushing phellinus igniarius growing in Changbai Mountain region into powder; adding water to the phellinus igniarius powder, and uniformly stirring; boiling for 2h in a boiling water bath to obtain a mixed solution; cooling the mixed solution; centrifuging at superspeed; collecting supernate; filtering the supernate to obtain the phellinus igniarius aqueous extract; sterilizing under the temperature of 121 DEG C and high pressure to obtain the phellinus igniarius aqueous extract. Compared with colchicine, the phellinus igniarius aqueous extract has the effect of reducing depositingtrioxypurine in articular cavity, so that the trioxypurine content in a human body can be reduced through the phellinus igniarius aqueous extract, to treat gout.

Description

technical field [0001] The invention belongs to the technical field of Phellinus Phellinus extract and its application, in particular to a Phellinus Phellinus water extract and its anti-uric acid application. Background technique [0002] Gout is a common arthritic disease associated with joint pain, fatigue and high fever. The increase of uric acid level is the biochemical basis of gout. Because primates lost uricolytic enzymes during evolution, they could not further decompose the end product of purine metabolism (uric acid) into water-soluble allantoin, resulting in uric acid in the blood Increased, thus causing the deposition of urate in the tissue, making gout a more special human disease. According to research, the prevalence of gout is 2%, especially in men over 40 years old with metabolic syndrome. [0003] It is generally believed that gout is caused by chronic hyperuricemia (serum uric acid [UA] level>6.8mg / dL), sodium urate is deposited in the joints, asympto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07A61Q19/06
CPCA61K36/07A61K2236/331A61Q19/06
Inventor 施维朱玉花
Owner JILIN UNIV
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