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Method for culturing atrial muscle cells of rats

A culture method and technology for muscle cells, applied in cell dissociation methods, tissue culture, animal cells, etc., can solve the problems of difficult passage and easily damaged atrial myocytes, and achieve enhanced protection, complete digestion and separation, and cells. Vibrant effect

Inactive Publication Date: 2018-08-17
LABREAL BIOTECH KUNMING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, atrial myocytes are easily damaged during separation, digestion, centrifugation, etc., and they rarely divide and are not easy to be passaged. Therefore, it is necessary to find a culture method for atrial myocytes that can increase the number of atrial myocytes and have a high survival rate.

Method used

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  • Method for culturing atrial muscle cells of rats
  • Method for culturing atrial muscle cells of rats

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A culture method for rat atrial myocytes, comprising the following steps:

[0026] (1) Material collection: Take a live rat heart, keep it beating after the heart is removed, quickly perfuse sterile PBS buffer solution from the left ventricle, wash the blood in the heart, and open the right atrium with microsurgical instruments under the ultra-clean table The right atrial appendage of the right atrial appendage, peel off the inner wall film of the atrium, peel off the muscle layer with micro-scissors, and cut it into 1mm pieces with micro-scissors 3 volume size tissue block;

[0027] (2) digestion

[0028] A. Trypsin digestion: Digest the above cut tissue mass with 0.25% trypsin-EDTA for 1min, the digestion temperature is 37°C, shake at 50rpm during the digestion process, then add 1.2 times the volume of the digestion solution containing 20% ​​fetal bovine serum DMEM / F12 medium, stop digestion;

[0029] B. Comprehensive enzyme digestion: Centrifuge at 800rpm for 3 mi...

Embodiment 2

[0034] A culture method for rat atrial myocytes, comprising the following steps:

[0035] (1) Material collection: Take a live rat heart, keep it beating after the heart is removed, quickly perfuse sterile PBS buffer solution from the left ventricle, wash the blood in the heart, and open the right atrium with microsurgical instruments under the ultra-clean table The right atrial appendage of the right atrial appendage, peel off the inner wall film of the atrium, peel off the muscle layer with micro-scissors, and cut it into 1mm pieces with micro-scissors 3 volume size tissue block;

[0036] (2) digestion

[0037] A. Trypsin digestion: Digest the above cut tissue mass with 0.25% trypsin-EDTA for 1min, the digestion temperature is 37°C, shake at 50rpm during the digestion process, then add 1.5 times the volume of the digestion solution containing 20% ​​fetal bovine serum DMEM / F12 medium, stop digestion;

[0038] B. Comprehensive enzyme digestion: Centrifuge at 800rpm for 3 mi...

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PUM

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Abstract

The invention discloses a method for culturing atrial muscle cells of rats, and belongs to the technical field of cell culture. The method includes steps of material acquisition, digestion, separation, centrifugation, inoculated culture and the like. The method for culturing the atrial muscle cells of the rats has the advantages that the method is low in cost and high in efficiency and is stable,separated cells are good in viability and high in purity, and the atrial muscle cells are high in quantity and yield; effects of protecting the cells can be improved by the aid of the method in culture procedures, accordingly, the viability of the cells can be improved, and the cells are high in survival rate; medicines such as penicillin and Brdu are omitted in the culture procedures, and accordingly potential impact of the penicillin and the Brdu on follow-up research can be prevented.

Description

technical field [0001] The invention belongs to the technical field of cell culture, in particular to a method for culturing rat atrial myocytes. Background technique [0002] The atrial myocyte culture model can eliminate the influence of various interfering factors in the in vivo experiment, and study the effect of the target factor on the atrial myocytes in a specific environment. The use of atrial myocytes cultured in vitro to establish a variety of pathophysiological models is conducive to the study of the mechanism and prevention of cardiovascular diseases from the level of cell and molecular biology. However, atrial myocytes are easily damaged during separation, digestion, centrifugation, etc., and they rarely divide and are not easy to be passaged. Therefore, it is necessary to find a culture method of atrial myocytes that can increase the number of atrial myocytes and have a high survival rate. Contents of the invention [0003] In order to solve the technical pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
CPCC12N5/0657C12N2509/00
Inventor 贺永胜李慧范振峰
Owner LABREAL BIOTECH KUNMING CO LTD
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