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Wright-Giemsa staining reagent and application method thereof

A technology for dyeing reagents and reagent bottles, applied in the field of biological reagents, can solve the problems of complicated preparation methods of dyes, poor dyeing effect of dyes, long dyeing time, etc., and achieves simple and convenient preparation methods, excellent effects and short dyeing time. Effect

Inactive Publication Date: 2018-08-17
天津见康华美医学诊断技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the technical defect of prior art, provides a kind of Wright-Giemsa staining reagent and its application method, to solve the problem of smears of specimens such as spinal fluid cells, peripheral blood cells, pleural and ascites cells, and cerebrospinal fluid cells in the prior art. The technical problem of poor dyeing effect of dyeing agent
[0004] Another technical problem to be solved by the present invention is that the dyeing time required by related dyes in the prior art is longer
[0005] Another technical problem to be solved by the present invention is that the preparation method of related dyes in the prior art is loaded down with trivial details

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A Wright-Giemsa staining reagent prepared by the following method:

[0028] 1) Mix Wright dye and Giemsa dye at a ratio of 5:1 by weight, and take methanol for use at a ratio of 1:500 (g:mL) of Wright dye to methanol;

[0029] 2) Grinding the mixture of Wright dye and Giemsa dye into powder in a mortar;

[0030] 3) Add 1 / 10 of the total volume of methanol to the powder and continue grinding for 20 minutes, then pour the dissolved dye solution into a brown reagent bottle to obtain undissolved solids;

[0031] 4) Repeat step 3) until all the solids are dissolved, and add all the remaining methanol into the brown reagent bottle;

[0032] 5) Sealed and protected from light at room temperature for 1 month.

[0033] Wherein, the term of validity of the Wright-Giemsa staining reagent is 5 years.

[0034] A method for using the Wright-Giemsa staining reagent, comprising the steps of: dripping 0.25mL of the Wright-Giemsa staining solution on the smear of the biological specim...

Embodiment 2

[0043] A Wright-Giemsa staining reagent prepared by the following method:

[0044] 1) Mix Wright dye and Giemsa dye at a ratio of 5:1 by weight, and take methanol for use at a ratio of 1:500 (g:mL) of Wright dye to methanol;

[0045] 2) Grinding the mixture of Wright dye and Giemsa dye into powder in a mortar;

[0046] 3) Add 1 / 10 of the total volume of methanol to the powder and continue grinding for 20 minutes, then pour the dissolved dye solution into a brown reagent bottle to obtain undissolved solids;

[0047] 4) Repeat step 3) until all the solids are dissolved, and add all the remaining methanol into the brown reagent bottle;

[0048] 5) Sealed and protected from light at room temperature for 1 month.

[0049] A method for using the Wright-Giemsa staining reagent, comprising the steps of: dripping 0.25 mL of the Wright-Giemsa staining solution on the smear of the pleural and ascites cells, adding 1 mL of phosphate buffer working solution dropwise after 30 s, blowing ...

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Abstract

The invention provides a Wright-Giemsa staining reagent and an application method thereof. According to a technical scheme in the invention, the staining reagent is characterized in the aspect of a preparation method; the preparation method is simple and convenient and can be implemented in a short time; and the obtained staining reagent has long service life in a specific preservation environment. On such a basis, a method for staining a biomaterial smear with the staining reagent is novelly designed; and the method comprises the following steps: staining the smear with 0.25 mL of the staining reagent for 30 s, then dropwise adding 1 mL of a phosphate buffer, maintaining the staining reagent in the phosphate buffer for 20 min, carrying out washing with water and then carrying out drying in the air so as to complete staining. The staining reagent of the invention is simple to prepare, short in staining time, excellent in effect, capable of realizing easy identification of various cells, and mainly applicable to staining detection of specimen smears of bone marrow fluid cells, peripheral blood cells, hydrothorax and ascite cells, cerebrospinal fluid cells and the like.

Description

technical field [0001] The invention relates to the technical field of biological reagents, and further relates to the preparation and application of biological material stains, in particular to a Wright-Giemsa staining reagent and a use method thereof. Background technique [0002] Due to the poor staining effect of commercially available reagents, which may lead to errors in cell classification or interpretation, the formula and staining time were adjusted accordingly. Wright dye is composed of acid dye eosin and basic dye methylene blue. It has good staining effect on cytoplasmic components and neutral granules, but Wright dye is not as good as Giemsa dye on nucleus staining. Giemsa dye, composed of azure and eosin, stains nuclei well. On the one hand, methanol can dissociate eosin into ionic E- and methylene blue into ionic M+; on the other hand, it can fix cells. Wright-Giemsa stain has both physical adsorption and chemical affinity for cell staining. Many components ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 汝昆田欣王占龙蔺亚妮
Owner 天津见康华美医学诊断技术有限公司
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