Room-temperature transport liquid for MSC (mesenchymal stem cells)

A technology of quality stem cells and transport fluid, which is applied in the field of cell transport fluid, can solve the problems of unclear components of cryopreservation fluid, decreased cell activity, and low cell number, and achieve excellent biological characteristics preservation effect, convenient preparation, and simple components Effect

Inactive Publication Date: 2018-08-24
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the use of liquid nitrogen transportation can store a large number of cells in a specific liquid nitrogen container before transportation, but there are certain safety hazards, and the cost is high. The composition of the cryopreservation solution is not clear, and generally contains different proportions of serum. cause a certain impact, and at the same time the activity of froze...

Method used

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  • Room-temperature transport liquid for MSC (mesenchymal stem cells)
  • Room-temperature transport liquid for MSC (mesenchymal stem cells)
  • Room-temperature transport liquid for MSC (mesenchymal stem cells)

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A normal temperature transport solution for mesenchymal stem cells, which consists of 25% 0.2% trehalose solution, 40% red blood cell storage solution, 1% 0.2% heparin calcium and 34% human serum albumin injection according to the mass percentage of raw materials Mix at room temperature to form a uniform composition.

Embodiment 2

[0022] A normal temperature transport solution for mesenchymal stem cells, which consists of 40% 0.2% trehalose solution, 20% red blood cell storage solution, 10% 0.2% heparin calcium and 30% human serum albumin injection according to the mass percentage of raw materials Mix at room temperature to form a uniform composition.

Embodiment 3

[0024] A normal temperature transport solution for mesenchymal stem cells, which consists of 30% 0.2% trehalose solution, 25% red blood cell storage solution, 5% 0.2% heparin calcium and 40% human serum albumin injection according to the mass percentage of raw materials Mix at room temperature to form a uniform composition.

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Abstract

The invention discloses a room-temperature transport liquid for MSC (mesenchymal stem cells). The room-temperature transport liquid comprises raw materials in percentage by mass as follows: 25%-40% ofa trehalose liquor, 20%-40% of a red blood cell stock liquor, 1%-10% of heparin calcium and 20%-45% of a human albumin injection. Various defects of traditional MSC transport are overcome, room-temperature mass transport is realized, huge waste of culture media and liquid nitrogen in the traditional transport mode is avoided, and cost is reduced effectively. Meanwhile, the room-temperature transport liquid contains simple components, is convenient to prepare and is pollution-free, and has excellent biological characteristic preservation effect on the MSC.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a cell transport fluid. Background technique [0002] Recent advances in cell therapy, regenerative medicine, and stem cell research have significantly increased the need for various types of readily available cell suspensions, especially for mesenchymal stem cells (MSCs). Mesenchymal stem cells are multipotent stromal cells isolated from adult and fetal tissues, which are defined as adherent fibroblast-like cells. MSCs are derived from the early mesoderm of development and have a high degree of self-renewal ability and multilineage differentiation potential. In vitro induction can differentiate into various types of cells, such as osteoblasts, chondrocytes, nerve cells, adipocytes and cardiogenic cells. [0003] It is a widely accepted fact that mesenchymal stem cells have immunosuppressive and immunomodulatory functions. Due to their ability to modulate the immune resp...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 赵姝灿陈志胜郑桂纯张晓芳
Owner FOSHAN UNIVERSITY
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