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Genetically engineered natural killer cell product for treating tumor

A technology of natural killer cells and genetic engineering, applied in the field of biopharmaceutical products, can solve problems such as reducing the effect of immunotherapy, and achieve the effect of good anti-cancer function and safety

Inactive Publication Date: 2018-09-14
SYNO SHENZHEN BIOMEDICAL RES CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Unfortunately, while improving safety, microradiation treatment also reduces the immunotherapeutic efficacy of the product

Method used

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  • Genetically engineered natural killer cell product for treating tumor
  • Genetically engineered natural killer cell product for treating tumor
  • Genetically engineered natural killer cell product for treating tumor

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Embodiment Construction

[0031] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0032] 1. Construction and expression of IL-2-T2A-HSV-tk vector genetic engineering plasmid vector

[0033] Cloned expression plasmids synthesized with IL-2 cDNA and HSV-tk cDNA gene fragments were ordered from American Life Technologies. Expression plasmids are lyophilized. The synthesized IL-2 cDNA was cloned into pcDNA3.1 / Neo (InvitrogenV79020) vector to construct IL-2 expression plasmid vector. The synthetic HSV-tk cDNA gene fragment was cloned into the pcDNA3.1 / Hygro (Invitrogen V870-20) vector to construct the suicide gene plasmid vector. The gene fragments of IL-2 cDNA and HSV-tk cDNA wer...

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Abstract

Provided are genetically engineered natural killer cell products for treating tumors. The cell products are genetically engineered to stably transduce human IL-2 (Interleukin-2) and HSV-tk (herpes simlex virus-thymidine kinase) genes into natural killer cells (NK). The cell products are allogeneic cell products with good stability, anti-tumor effect and safety and can be produced on a large scalewithout the adding of the IL-2. The genetically engineered natural killer cell products (NK) can be prepared into clinical grade gene cell therapeutic products for the treatment and prevention of various malignant tumors in humans.

Description

technical field [0001] The invention belongs to the field of biopharmaceutical products, in particular to a genetically engineered natural killer cell product for treating tumors. Background technique [0002] Tumor biological therapy is the fourth major treatment method after surgery, radiotherapy and chemotherapy. Since the development of traditional surgery, radiotherapy and chemotherapy has entered a plateau, more and more people are focusing on the biological treatment of tumors. Gene and cellular immunotherapy for cancer is at the forefront of tumor biotherapy. Compared with traditional treatment methods such as chemotherapy and radiotherapy, it has the advantages of relatively less toxic and side effects and significant curative effect. Especially in the past few years, the medical community has shown great interest in cellular immunotherapy for hematologic malignancies. Cellular immunotherapy and pre-chemotherapy and radiotherapy have non-crossover clinical effects...

Claims

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Application Information

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IPC IPC(8): C12N5/10A61K35/17A61P35/00
CPCA61K35/17A61P35/00C12N5/0646C12N2510/00
Inventor 张书元徐卫
Owner SYNO SHENZHEN BIOMEDICAL RES CO LTD
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