A method for analyzing the marker metabolites of maca based on liquid chromatography-mass spectrometry metabolomics

A technology of metabolites and metabolomics, which is applied in the field of analysis of Maca's iconic metabolites based on liquid mass spectrometry metabolomics, can solve problems such as differences in content, and achieve the effect of simple operation

Active Publication Date: 2020-08-04
YANTAI NEW ERA HEALTH IND
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The HPLC spectrum shows that all 15 batches of maca contain these three glucosinolates, but the contents are different

Method used

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  • A method for analyzing the marker metabolites of maca based on liquid chromatography-mass spectrometry metabolomics
  • A method for analyzing the marker metabolites of maca based on liquid chromatography-mass spectrometry metabolomics
  • A method for analyzing the marker metabolites of maca based on liquid chromatography-mass spectrometry metabolomics

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Embodiment 1

[0060] A method for analyzing maca marker metabolites based on liquid mass spectrometry metabolomics, the steps are as follows:

[0061] (1) Preparation of maca extract

[0062] Weigh 1g of each powder sample of 20 different phenotypes of maca, add 20mL of methanol to mix, respectively, after ultrasonic extraction for 20min, centrifuge at 3900rpm for 20min, and the obtained supernatant passes through a filter membrane with a pore size of 0.22μm to obtain the maca extract. liquid;

[0063] (2) Maca extract pretreatment

[0064] The Maca extract in step (1) is diluted 20 times with methanol, and the diluted Maca extract passes through a filter membrane with a pore size of 0.22 μm to obtain a filtrate;

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Abstract

The invention relates to a method for analyzing maca marker metabolites based on liquid mass spectrometry metabolomics. The present invention extracts maca metabolites through methanol, performs UHPLC-Q Exactive Orbitrap LC-MS analysis after pretreatment, uses Compound Discover to process data, and then imports data into SIMCA14.0 software, uses principal component analysis (PCA), partial least binary Multiply discriminant analysis (PLS-DA) and orthogonal-partial least squares discriminant analysis (OPLS-DA) to analyze the data to find differential metabolites; use the permutation permutation test method to test the effectiveness of the PLS-DA model, and the results show that the model is established efficient. The analysis method of the invention is simple to operate, and the metabolites of different phenotypes of maca are searched, so as to be used for distinguishing and identifying different phenotypes of maca.

Description

technical field [0001] The invention belongs to the technical field of detection and analysis, and in particular relates to a method for analyzing maca marker metabolites based on liquid mass spectrometry metabolomics. Background technique [0002] Maca (Lepidium meyenii Walp.), a plant of the genus Lepidium of the Cruciferae (Cruciferae), is native to the South American Andes at an altitude of 3,500 to 4,500 meters. It has been cultivated for more than 2,000 years and has edible and medicinal value. . Since the 1990s, the United States, Japan, Germany and many other countries have tried to introduce and cultivate Maca. In 2002, Maca was introduced into China. Over the past ten years, this plant has been successfully introduced and cultivated in many regions of China, such as Yunnan, Xinjiang, Jilin and Sichuan. [0003] The nutritional content of Maca is reasonable and various, mainly including: protein, various amino acids, fatty acids, cellulose, sugars, calcium, zinc,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 杨长军耿越王桐刘梅徐宏楠梁晓庆仲米存
Owner YANTAI NEW ERA HEALTH IND
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