A method for analyzing the marker metabolites of maca based on liquid chromatography-mass spectrometry metabolomics

A technology of metabolites and metabolomics, which is applied in the field of analysis of Maca's iconic metabolites based on liquid mass spectrometry metabolomics, can solve problems such as differences in content, and achieve the effect of simple operation
CN108535375BActive Publication Date: 2020-08-04YANTAI NEW ERA HEALTH IND
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YANTAI NEW ERA HEALTH IND
Publication Date
2020-08-04

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Abstract

The invention relates to a method for analyzing maca marker metabolites based on liquid mass spectrometry metabolomics. The present invention extracts maca metabolites through methanol, performs UHPLC-Q Exactive Orbitrap LC-MS analysis after pretreatment, uses Compound Discover to process data, and then imports data into SIMCA14.0 software, uses principal component analysis (PCA), partial least binary Multiply discriminant analysis (PLS-DA) and orthogonal-partial least squares discriminant analysis (OPLS-DA) to analyze the data to find differential metabolites; use the permutation permutation test method to test the effectiveness of the PLS-DA model, and the results show that the model is established efficient. The analysis method of the invention is simple to operate, and the metabolites of different phenotypes of maca are searched, so as to be used for distinguishing and identifying different phenotypes of maca.
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Description

technical field

[0001] The invention belongs to the technical field of detection and analysis, and in particular relates to a method for analyzing maca marker metabolites based on liquid mass spectrometry metabolomics. Background technique

[0002] Maca (Lepidium meyenii Walp.), a plant of the genus Lepidium of the Cruciferae (Cruciferae), is native to the South American Andes at an altitude of 3,500 to 4,500 meters. It has been cultivated for more than 2,000 years and has edible and medicinal value. . Since the 1990s, the United States, Japan, Germany and many other countries have tried to introduce and cultivate Maca. In 2002, Maca was introduced into China. Over the past ten years, this plant has been successfully introduced and cultivated in many regions of China, such as Yunnan, Xinjiang, Jilin and Sichuan.

[0003] The nutritional content of Maca is reasonable and various, mainly including: protein, various amino acids, fatty acids, cellulose, sugars, calcium, zinc,...

Claims

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