A method for analyzing the marker metabolites of maca based on liquid chromatography-mass spectrometry metabolomics
A technology of metabolites and metabolomics, which is applied in the field of analysis of Maca's iconic metabolites based on liquid mass spectrometry metabolomics, can solve problems such as differences in content, and achieve the effect of simple operation
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[0060] A method for analyzing maca marker metabolites based on liquid mass spectrometry metabolomics, the steps are as follows:
[0061] (1) Preparation of maca extract
[0062] Weigh 1g of each powder sample of 20 different phenotypes of maca, add 20mL of methanol to mix, respectively, after ultrasonic extraction for 20min, centrifuge at 3900rpm for 20min, and the obtained supernatant passes through a filter membrane with a pore size of 0.22μm to obtain the maca extract. liquid;
[0063] (2) Maca extract pretreatment
[0064] The Maca extract in step (1) is diluted 20 times with methanol, and the diluted Maca extract passes through a filter membrane with a pore size of 0.22 μm to obtain a filtrate;
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