A method for establishing an experimental plateau intestinal barrier injury rat model
A technology of intestinal barrier and rat model, applied in the field of medicine, can solve the problems of restricting the mechanism of disease occurrence, the exploration of the development process and the evaluation of the treatment effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] Example 1 Constructing an animal model
[0030] (1) Plain adaptive training stage
[0031] Before the exercise intervention, the two groups of animals were raised in the experimental site (Chengdu, May, altitude about 500m, temperature 24C°) for 1 week to eliminate the disturbance of the unfamiliar environment to the animals after the start of the experiment, no restriction on drinking water, and natural light. On the 8th day, all rats started adaptive training on the animal treadmill at 8:00 in the morning, in order to avoid the cooperation obstacle caused by the animals not familiar with the experimental equipment during the formal exercise intervention. The specific implementation method is: use the ZH-PT type animal experiment On the treadmill (10 tracks in total), adjust the conveyor belt slope to 0°, the speed at 8m / min, the time is 30min, and then rest for 30min, during which time you can eat and drink freely. Repeat the training 8 times in this way, the trainin...
Embodiment 2
[0036] Example 2 Detection of animal models
[0037] (1) Sample collection
[0038] After the experiment, group C collected materials in the plain environment, and group H quickly collected materials at a simulated altitude of 4000m. The weight of the rats was accurately weighed, and anesthetized by intraperitoneal injection of 10% ureose at 1 g / kg·BW. After the corneal reflex disappeared, the rats were fixed on the animal operating table in a supine position, and strictly aseptically operated. 20 rats were taken from each group, and animal blood, liver, spleen, mesenteric lymph nodes, and jejunum were collected in an ultra-clean workbench, and stored in liquid nitrogen for testing. The remaining 15 rats in each group were anesthetized in the same way, and a 2cm-long section of jejunum was cut 10cm away from the pylorus, cut longitudinally and trimmed into 1mm×1mm×1mm specimens, immersed in corresponding fixatives, and pathologically observed later.
[0039] (2) Bacterial tr...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com