Sample crystal particle generating system and method

A technology for generating systems and samples, applied in biochemical equipment and methods, biochemical instruments, biomass post-processing, etc., to facilitate rapid assembly and integration, eliminate the possibility of contamination, and have a simple structure.

Active Publication Date: 2018-09-28
MEDCAPTAIN MEDICAL TECH
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  • Abstract
  • Description
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Problems solved by technology

[0004] In addition, due to the inhibitory effect of PCR amplification products on enzyme-catalyzed reactions, the current g

Method used

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  • Sample crystal particle generating system and method
  • Sample crystal particle generating system and method
  • Sample crystal particle generating system and method

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Embodiment 1

[0044] like Figure 7 , Figure 8 , Figure 9 , Figure 10 The illustrated embodiment is a sample crystal particle generating system, comprising a needle holder 102, an air pressure tube 103 connected to the needle holder, an air pressure needle 104, a sample crystal particle generator 101 and a lower part of the generator for accommodating the sample crystal particles The heat-conducting container 105; the upper end and the lower end of the heat-conducting container are respectively provided with a first sealing cover 151 and a sealing sleeve 152, the first sealing cover is provided with an exhaust port 153, and the exhaust port is provided with a one-way valve 154; the air pressure needle and the sample crystal The particle generator is connected, and the heat transfer container is provided with crystal particle carrier oil, such as Figure 7 As shown, the height of the oil surface of the crystal particle carrier oil is higher than the height of each micro flow channel of...

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Abstract

The invention discloses a sample crystal particle generating system and method. The sample crystal particle generating system comprises a syringe needle fixing frame, an air pressure pipeline connected with the syringe needle fixing frame, an air pressure syringe needle, a sample crystal particle generator and a heat conducting container for containing the lower portion of the sample crystal particle generator. A first sealing cover and a sealing sleeve are arranged at the upper end and the lower end of the heat conducting container correspondingly; an air exhaust opening is formed in the first sealing cover, and a one-way valve is arranged at the air exhaust opening; the air pressure syringe needle is connected with the sample crystal particle generator; crystal particle carrier oil is arranged in the heat conducting container; the lower portion of the sample crystal particle generator is located in the heat conducting container; the oil surface height of the crystal particle carrieroil is larger than the height of positions where all micro flow channels of the sample crystal particle generator are located; and a constant-current and constant-pressure pump is arranged on the airpressure pipeline. The sample crystal particle generating system has the characteristics of excellent stability and reproducibility, ultra-high sensitivity, extreme precision and low cost.

Description

technical field [0001] The invention relates to the technical field of gene molecular diagnostic reagent equipment, in particular to a sample crystal particle generation system and method for ensuring that samples in a heat-conducting container are isolated from the outside world. Background technique [0002] At present, fluorescence quantitative PCR (Fluorescence Quantitative Polymerase Chain Reaction, qPCR) has developed into a key routine technology in the field of in vitro molecular diagnosis, which has greatly promoted the development of life science in the field of health care. However, fluorescence quantitative PCR quantification is only relative quantification, and its accuracy and reproducibility still cannot meet the development needs of the current field of in vitro molecular diagnostic systems and biological genetics research. However, in many projects such as liquid biopsy and NIPT, there are some technical bottlenecks. For example, the content of tumor circula...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/6806
CPCC12Q1/6806C12Q2565/629
Inventor 王亚琴
Owner MEDCAPTAIN MEDICAL TECH
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