Sample crystal particle generating system and method

Abstract

The invention discloses a sample crystal particle generating system and method. The sample crystal particle generating system comprises a syringe needle fixing frame, an air pressure pipeline connected with the syringe needle fixing frame, an air pressure syringe needle, a sample crystal particle generator and a heat conducting container for containing the lower portion of the sample crystal particle generator. A first sealing cover and a sealing sleeve are arranged at the upper end and the lower end of the heat conducting container correspondingly; an air exhaust opening is formed in the first sealing cover, and a one-way valve is arranged at the air exhaust opening; the air pressure syringe needle is connected with the sample crystal particle generator; crystal particle carrier oil is arranged in the heat conducting container; the lower portion of the sample crystal particle generator is located in the heat conducting container; the oil surface height of the crystal particle carrieroil is larger than the height of positions where all micro flow channels of the sample crystal particle generator are located; and a constant-current and constant-pressure pump is arranged on the airpressure pipeline. The sample crystal particle generating system has the characteristics of excellent stability and reproducibility, ultra-high sensitivity, extreme precision and low cost.

Description

technical field [0001] The invention relates to the technical field of gene molecular diagnostic reagent equipment, in particular to a sample crystal particle generation system and method for ensuring that samples in a heat-conducting container are isolated from the outside world. Background technique [0002] At present, fluorescence quantitative PCR (Fluorescence Quantitative Polymerase Chain Reaction, qPCR) has developed into a key routine technology in the field of in vitro molecular diagnosis, which has greatly promoted the development of life science in the field of health care. However, fluorescence quantitative PCR quantification is only relative quantification, and its accuracy and reproducibility still cannot meet the development needs of the current field of in vitro molecular diagnostic systems and biological genetics research. However, in many projects such as liquid biopsy and NIPT, there are some technical bottlenecks. For example, the content of tumor circula...

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Problems solved by technology

[0004] In addition, due to the inhibitory effect of PCR amplification products on enzyme-catalyzed reactions, the current gene variation detection method of qPCR technology is often powerless for low-abundance tumor gene variation in somatic cells

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