Sample crystal particle detection system and method

Abstract

The invention discloses a sample crystal particle detection system and method. The system comprises a controller, M sample crystal particle generators, a constant current constant voltage device, M heat-conducting containers filled with crystal particle carrier oil, a crystal particle collection device connected with the bottom of one heat-conducting container, a crystal particle light path detector, a plurality of lasers of which wavelengths are sequentially increased, exciting light filter assemblies, a received light filter assembly, a photoelectric signal collector, a PC and a temperaturecontrol device, wherein the lasers are positioned on one side of the crystal particle light path detector and arranged in sequence; the exciting light filter assemblies are arranged at light outlets of the various lasers; the received light filter assembly is positioned on the other side of the crystal particle light path detector; the photoelectric signal collector is connected with the receivedlight filter assembly. The sample crystal particle detection system disclosed by the invention has the characteristics that an optical crosstalk phenomenon does not occur between light paths of the wave bands, the top-pressing and bottom-arranging structure is adopted, and dead space does not remain in the chip.

Description

technical field [0001] The invention relates to the technical field of gene molecular diagnostic reagent equipment, in particular to a sample crystal particle detection system and method with excellent stability and reproducibility, ultra-high sensitivity, and extreme accuracy. Background technique [0002] At present, fluorescence quantitative PCR (Fluorescence Quantitative Polymerase Chain Reaction, qPCR) has developed into a key conventional technology in the field of in vitro molecular diagnosis, which has greatly promoted the development of life science in the field of health care. However, fluorescence quantitative PCR quantification is only relative quantification, and its accuracy and reproducibility still cannot meet the development needs of the current field of in vitro molecular diagnostic systems and biological genetics research. However, in many projects such as liquid biopsy and NIPT, there are some technical bottlenecks. For example, the content of tumor circu...

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Problems solved by technology

[0004] In addition, due to the inhibitory effect of PCR amplification products on enzyme-catalyzed reactions, the current gene variation detection method of qPCR technology is often powerless for low-abundance tumor gene variation in somatic cells

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