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Rooting culture method for tissue culture seedlings of melaleuca alternifolia

A technology of Melaleuca alternifolia and rooting culture, applied in the field of plant tissue culture, to achieve the effects of high rooting rate, favorable reproduction and prevention of browning

Inactive Publication Date: 2018-10-16
浙江互叶生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And how to select suitable rooting medium and suitable culture method, suppress the browning of Melaleuca alternifolia effectively in the rooting culture stage, there is no report yet

Method used

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  • Rooting culture method for tissue culture seedlings of melaleuca alternifolia
  • Rooting culture method for tissue culture seedlings of melaleuca alternifolia

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for rooting cultivation of Melaleuca alternifolia tissue culture seedlings, comprising the steps of:

[0026] (1) Insert the explants of Melaleuca alternifolia into the induction medium after disinfection, and carry out the induction culture for 20 days. The induction medium consists of: MS+1.0mg / L 6-BA+0.3mg / L NAA, The pH of the induction medium is 5.7.

[0027] (2) The primordial buds of induced culture are transferred to the subculture medium, and the subculture medium is carried out for 35 days. The composition of the subculture medium is: MS+1.5mg / L 6-BA+1.0mg / L IAA. The pH of the subculture medium is 5.7.

[0028] (3) Cut single buds from the mother seedlings of subculture, transfer 1-2cm of single buds to the rooting medium, carry out rooting culture, cultivate in the dark for 7d, and then cultivate in light for 25d, and the light-dark cycle of light culture is 16 / 8h; the composition of the rooting medium is: 1 / 2MS+0.25mg / L NAA+1..25mg / L ABT-1, and th...

Embodiment 2

[0030] A method for rooting cultivation of Melaleuca alternifolia tissue culture seedlings, comprising the steps of:

[0031] (1) Insert the explants of Melaleuca alternifolia into the induction medium after disinfection, and carry out the induction culture for 30 days. The induction medium consists of: MS+1.5mg / L 6-BA+0.5mg / L NAA, The pH of the induction medium is 5.8.

[0032] (2) The primordial buds of induced culture are transferred to the subculture medium, and the subculture medium is carried out for 38 days. The composition of the subculture medium is: MS+2mg / L 6-BA+1.5mg / L IAA, the The pH of the subculture medium was 5.8.

[0033] (3) Cut single buds from the mother seedlings of subculture, transfer 1-2cm of single buds to the rooting medium, carry out rooting culture, cultivate in the dark for 7d, and then cultivate in light for 25d, and the light-dark cycle of light culture is 16 / 8h; the composition of the rooting medium is: 1 / 2MS+0.2mg / L NAA+1.5mg / L ABT-1, and th...

Embodiment 3

[0035] A method for rooting cultivation of Melaleuca alternifolia tissue culture seedlings, comprising the steps of:

[0036] (1) Insert the explants of Melaleuca alternifolia into the induction medium after disinfection, and carry out the induction culture for 25 days. The induction medium consists of: MS+1.5mg / L 6-BA+0.4mg / L NAA, The pH of the induction medium is 5.6.

[0037] (2) The primordial buds of the induced culture are transferred to the subculture medium, and the subculture medium is carried out for 40 days. The composition of the subculture medium is: MS+2mg / L 6-BA+1.0mg / L IAA, and the The pH of the subculture medium was 5.6.

[0038] (3) Cut single buds from the mother seedlings of subculture, transfer 1-2cm of single buds to the rooting medium, carry out rooting culture, cultivate in the dark for 7d, and then cultivate in light for 25d, and the light-dark cycle of light culture is 16 / 8h; the composition of the rooting medium is: 1 / 2MS+0.3mg / L NAA+1.0mg / L ABT-1...

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Abstract

The invention discloses a rooting culture method for tissue culture seedlings of melaleuca alternifolia. The rooting culture method comprises the following steps: (1) sterilizing a melaleuca alternifolia explant, inserting the explant into an induction medium, and conducting induction culture to obtain a primary bud; (2) transferring the primary bud of the induction culture to a subculture mediumfor subculture to obtain a subculture mother seedling; and (3) cutting a single bud from the subculture mother seedling, transferring the single bud of 1-2cm to a rooting culture medium for rooting culture, wherein the optimum composition of rooting culture medium is 1 / 2MS + 0.3mg / L NAA + 1.0mg / L ABT-1. The rooting culture method for tissue culture seedlings of melaleuca alternifolia, provided bythe invention, has an obvious effect of preventing the rooting culture of the tissue culture seedlings of melaleuca alternifolia from browning, has a high rooting rate, greatly improves the survival rate of tissue culture propagation of melaleuca alternifolia, is favorable for propagation of improved variety asexual seedlings, and has an important significance for large-scale production of tissueculture seedlings of melaleuca alternifolia.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a rooting culture method for tissue culture seedlings of Melaleuca alternifolia. Background technique [0002] Melaleuca ahemifolia is a genus of Melaleuca ahemfolia, native to Australia. Its leaf extract is commonly known as "tea tree essential oil". Because of its strong anti-inflammatory and bactericidal effects, it has been industrialized and widely used In the fields of medicine, cosmetics, and daily chemicals, my country has promoted planting in many provinces and cities such as Guangxi, Guangdong, and Fujian. [0003] Melaleuca alternifolia is mainly propagated by seeding, cutting and tissue culture. However, due to the current domestic planting varieties mixed, seed abortion, planting technical conditions and other problems, the germination rate and survival rate of Melaleuca alternifolia are relatively low. Low. If large-scale production is required, tissue c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 吴燕斌
Owner 浙江互叶生物科技有限公司
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