Rooting culture method for tissue culture seedlings of melaleuca alternifolia

A technology of Melaleuca alternifolia and rooting culture, applied in the field of plant tissue culture, to achieve the effects of high rooting rate, favorable reproduction and prevention of browning
CN108651283AInactive Publication Date: 2018-10-16浙江互叶生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
浙江互叶生物科技有限公司
Publication Date
2018-10-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a rooting culture method for tissue culture seedlings of melaleuca alternifolia. The rooting culture method comprises the following steps: (1) sterilizing a melaleuca alternifolia explant, inserting the explant into an induction medium, and conducting induction culture to obtain a primary bud; (2) transferring the primary bud of the induction culture to a subculture mediumfor subculture to obtain a subculture mother seedling; and (3) cutting a single bud from the subculture mother seedling, transferring the single bud of 1-2cm to a rooting culture medium for rooting culture, wherein the optimum composition of rooting culture medium is 1 / 2MS + 0.3mg / L NAA + 1.0mg / L ABT-1. The rooting culture method for tissue culture seedlings of melaleuca alternifolia, provided bythe invention, has an obvious effect of preventing the rooting culture of the tissue culture seedlings of melaleuca alternifolia from browning, has a high rooting rate, greatly improves the survival rate of tissue culture propagation of melaleuca alternifolia, is favorable for propagation of improved variety asexual seedlings, and has an important significance for large-scale production of tissueculture seedlings of melaleuca alternifolia.
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Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and relates to a rooting culture method for tissue culture seedlings of Melaleuca alternifolia. Background technique

[0002] Melaleuca ahemifolia is a genus of Melaleuca ahemfolia, native to Australia. Its leaf extract is commonly known as "tea tree essential oil". Because of its strong anti-inflammatory and bactericidal effects, it has been industrialized and widely used In the fields of medicine, cosmetics, and daily chemicals, my country has promoted planting in many provinces and cities such as Guangxi, Guangdong, and Fujian.

[0003] Melaleuca alternifolia is mainly propagated by seeding, cutting and tissue culture. However, due to the current domestic planting varieties mixed, seed abortion, planting technical conditions and other problems, the germination rate and survival rate of Melaleuca alternifolia are relatively low. Low. If large-scale production is required, tissue c...

Claims

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