Testing device and method for inducing calcium carbonate precipitation to solidify sandy soil by microorganisms in negative pressure environment

A test device, microbial technology, applied in the field of solidified sand, to achieve the effect of improving strength and simple operation

Pending Publication Date: 2018-11-06
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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  • Application Information

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Problems solved by technology

Aiming at the current technical status of the existing microbial-induced calcium carbonate precipitation sand-fixing technology, which is generally carried out under normal pressure conditions, the present invention intends to explore a method for microbial-induced calcium carbonate precipitation and solidification of sandy soil under negative pressure conditions

Method used

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  • Testing device and method for inducing calcium carbonate precipitation to solidify sandy soil by microorganisms in negative pressure environment
  • Testing device and method for inducing calcium carbonate precipitation to solidify sandy soil by microorganisms in negative pressure environment
  • Testing device and method for inducing calcium carbonate precipitation to solidify sandy soil by microorganisms in negative pressure environment

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Experimental program
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Effect test

Embodiment

[0040] Use the above-mentioned device to carry out the sand-fixing test, and the specific operation steps are as follows:

[0041] 1) Production of sandy soil test piece: put standard sand into the hollow cylindrical test piece mold 5, make 18 pieces in total, and place them on the porous plastic plate 8;

[0042] 2) Preparation of bonding solution: the nutrient solution uses water as a solvent and contains NH 4 Cl, NaHCO 3 , CaCl 2 .2H 2 O, CO (NH2 ) 2 , the NH 4 Cl, NaHCO 3 , CaCl 2 .2H 2 O, CO (NH 2 ) 2 The concentrations are 10.0g / L, 2.12g / L, 73.5g / L, 30.0g / L respectively; the pH value of the final bonding liquid will be adjusted to 6 by HCl or Na(OH);

[0043] 3) Bacterial culture solution preparation: the bacterial culture solution uses water as a solvent to contain (NH 4 ) 2 SO 4 , yeast powder, buffer solution, the pH value of the bacterial culture solution is 9.0, and the bacterial concentration OD 600 = 0.640, and the bacterial solution was mixed in the...

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Abstract

The invention discloses a testing device for inducing a calcium carbonate precipitation to solidify sandy soil by microorganisms in a negative pressure environment. The testing device comprises a culture box, an oxygen conveying device and a vacuumizing device. The invention further discloses a testing method for inducing the calcium carbonate precipitation to solidify the sandy soil by the microorganisms in the negative pressure environment. The method comprises the steps of manufacturing a sandy soil test piece, preparing a nutrient solution, mixing bacteria and the nutrient solution, debugging a culture environment, maintaining the aerobic negative pressure environment and the like. The disclosed testing method explores that under a negative pressure condition, along with the progressive increase of maintaining time, a rapid increased tendency appears in the strength of inducing the calcium carbonate precipitation to solidify the sandy soil by the microorganisms, and is superior tothe sand solidifying effect in a normal atmospheric pressure environment. The method has remarkable guiding significance in the actual application of negative pressure environmental engineering.

Description

technical field [0001] The invention belongs to the technical field of solidifying sandy soil, and in particular relates to a test device and method for microbe-induced calcium carbonate precipitation and solidifying sandy soil under a negative pressure environment. Background technique [0002] With the worldwide population growth and social development, the demand for construction land continues to increase. Most of the traditional chemical grouting materials and chemical curing agents contain toxic chemical substances, which often cause adverse effects on the environment. Microbe-induced solidification is a new type of soil solidification technology. Its essence is to induce some specific microorganisms to react with organic matter (such as urea) and combine with ion sources to generate carbonate crystals with bonding effect and insoluble Cemented or weakly cemented particles bond together to form a whole with a certain strength. [0003] CN201410671294 discloses a cons...

Claims

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Application Information

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IPC IPC(8): G01N33/24G01N1/28
CPCG01N1/28G01N33/24
Inventor 黄伟李琳温珂珺李俊杰邓晓佳董倩卜常明张俊珂刘世慧李阳苏佩东杜康
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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